| ○種別 (必須): |
| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Matsuda Hirofumi (日) (読)
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| 2. | (英) Hakamata Hideki (日) (読)
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| 3. | (英) Kawasaki Takashi (日) (読)
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| 4. | 坂下 直実
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| ○貢献度 (任意): |
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| 5. | (英) Miyazaki Akira (日) (読)
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| 6. | (英) Takahashi Kiyoshi (日) (読)
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| 7. | (英) Shichiri Motoaki (日) (読)
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| 8. | (英) Horiuchi Seikoh (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| ○題名 (必須): | □ | (英) Molecular cloning, functional expression and tissue distribution of rat acyl-coenzyme A:cholesterol acyltransferase. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Acyl-coenzyme A:cholesterol acyltransferase (ACAT) is an enzyme catalyzing the intracellular formation of cholesteryl esters from free cholesterol and fatty acyl-CoA. In the present study, we cloned rat ACAT cDNA and determined its tissue distribution. Rat ACAT cDNA, having a coding region of 1635 bp with its deduced protein sequence of 545 amino acids and two typical motifs such as signature sequences and leucine heptad motif, showed 83, 92 and 90% identity with human, mouse, and hamster ACAT, respectively. Expression of rat ACAT cDNA in A293 cells and CHO cells resulted in a 3.0 to 3.5-fold increase in the enzyme activity. Among twelve tissues examined, ACAT activity was highest in adrenal followed by liver and intestine while that of aorta was extremely low. The mRNA level was also the highest in adrenal among four tissues examined. However, in contrast to its high ACAT activity, the liver mRNA level was extremely low (adrenal > intestine > aorta > liver). Consistent with mRNA levels, immunohistochemical analyses with a specific ACAT antibody detected significant ACAT signals in adrenal and intestine but a negligible signal in liver. These results indicate that adrenal most abundantly expresses ACAT in rat. Furthermore, rat liver showed a high ACAT activity but an extremely low ACAT mRNA and negligible immunohistochemical reactivity, suggesting the presence of a structurally different ACAT protein(s) in rat liver. (日)
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| ○キーワード (推奨): | 1. | (英) Amino Acid Sequence (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Base Sequence (日) (読)
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| 4. | (英) CHO Cells (日) (読)
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| 5. | (英) Cloning, Molecular (日) (読)
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| 6. | (英) Cricetinae (日) (読)
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| 7. | (英) DNA Primers (日) (読)
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| 8. | (英) DNA, Complementary (日) (読)
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| 9. | 遺伝子発現 (gene expression)
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| 10. | (英) Humans (日) (読)
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| 11. | 免疫組織化学 (immunohistochemistry)
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| 12. | (英) Mice (日) (読)
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| 13. | (英) Molecular Sequence Data (日) (読)
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| 14. | (英) RNA, Messenger (日) (読)
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| 15. | (英) Rats (日) (読)
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| 16. | (英) Sequence Homology, Amino Acid (日) (読)
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| 17. | (英) Sterol O-Acyltransferase (日) (読)
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| 18. | (英) Tissue Distribution (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism ([Elsevier Science])
(pISSN: 0005-2760)
| ○ISSN (任意): | □ | 0005-2760
ISSN: 0005-2760
(pISSN: 0005-2760) Title: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid MetabolismPublisher: Elsevier B.V. (Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 1391
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 193 203
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1998年 3月 30日 (平成 10年 3月 30日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/S0005-2760(98)00007-1 (→Scopusで検索)
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| ○PMID (任意): | □ | 9555010 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): | □ | 2-s2.0-0032579978
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2-2-1, Kumamoto 860-0811, Japan. (日)
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| 2. | (英) Article.DataBankList.DataBank.DataBankName: GENBANK (日)
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| 3. | (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: D86373 (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 5. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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