『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=264444)

EID=264444EID:264444, Map:0, LastModified:2013年12月5日(木) 14:57:05, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[木戸 淳一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.木戸 淳一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.阿部 佳織
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Yatsushiro Shouki (日) 八代 聖基 (読) やつしろ しょうき
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6.永田 俊彦
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7.大家 利彦 (産業技術総合研究所健康工学研究センター)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8.田中 正人 ((独)産業技術総合研究所 健康工学研究センター)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
9.片岡 正俊
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip  (日)    [継承]
副題 (任意):
要約 (任意): (英) Gingival crevicular fluid (GCF) contains calprotectin, which appears to be a useful biomarker for periodontal diseases because of its high level in GCF from periodontally diseased pockets. To determine calprotectin in GCF that has a very small volume, sandwich enzyme-linked immunosorbent assay (ELISA) on a microchip was performed and its utility was estimated. Anti-calprotectin primary antibody was discharged on a microchip using a piezoelectric inkjet printing system. Calprotectin standard and calprotectin in GCF samples from eleven subjects were determined by the ELISA method with the prepared microchip and their values were compared with those obtained by conventional ELISA. Using the ELISA on a microchip, a reasonable standard curve of calprotectin protein (1.56-100 ng/mL) was obtained. Calprotectin in GCF samples was quantified and showed reasonable values in accordance with the condition of periodontal diseases. The values determined by the microchip method and conventional ELISA showed a significant linear relationship (R(2)=0.981). Calprotectin in GCF was determined using the ELISA on a microchip with high efficiency and this ELISA method for calprotectin determination may become a useful method for diagnosing periodontal diseases.  (日)    [継承]
キーワード (推奨): 1. (英) Aged (日) (読) [継承]
2. (英) Antibodies, Immobilized (日) (読) [継承]
3.校正 (calibration) [継承]
4. (英) Enzyme-Linked Immunosorbent Assay (日) (読) [継承]
5. (英) Female (日) (読) [継承]
6. (英) Gingival Crevicular Fluid (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Lab-On-A-Chip Devices (日) (読) [継承]
9. (英) Leukocyte L1 Antigen Complex (日) (読) [継承]
10. (英) Linear Models (日) (読) [継承]
11. (英) Male (日) (読) [継承]
12. (英) Microfluidic Analytical Techniques (日) (読) [継承]
13. (英) Middle Aged (日) (読) [継承]
14. (英) Periodontal Diseases (日) (読) [継承]
15. (英) Reference Standards (日) (読) [継承]
16. (英) Reproducibility of Results (日) (読) [継承]
17. (英) Sensitivity and Specificity (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Clinical Biochemistry (Canadian Society of Clinical Chemists)
(pISSN: 0009-9120, eISSN: 1873-2933)

ISSN (任意): 1873-2933
ISSN: 0009-9120 (pISSN: 0009-9120, eISSN: 1873-2933)
Title: Clinical biochemistry
Title(ISO): Clin Biochem
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 45 [継承]
(必須): 15 [継承]
(必須): 1239 1244 [継承]
都市 (任意):
年月日 (必須): 西暦 2012年 5月 17日 (平成 24年 5月 17日) [継承]
URL (任意):
DOI (任意): 10.1016/j.clinbiochem.2012.05.009    (→Scopusで検索) [継承]
PMID (任意): 22609893    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1016/j.clinbiochem.2012.05.009  (日)    [継承]
2.(英) Article.ELocationID: S0009-9120(12)00242-1  (日)    [継承]
3.(英) Article.Affiliation: Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. kido@dent.tokushima-u.ac.jp  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
5.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Jun-ichi Kido, Kaori Abe, Shouki Yatsushiro, Mika Bandou, Yuka Hiroshima, Toshihiko Nagata, Toshihiko Ooie, Masato Tanaka and Masatoshi Kataoka : Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip, Clinical Biochemistry, Vol.45, No.15, 1239-1244, 2012.
欧文冊子 ● Jun-ichi Kido, Kaori Abe, Shouki Yatsushiro, Mika Bandou, Yuka Hiroshima, Toshihiko Nagata, Toshihiko Ooie, Masato Tanaka and Masatoshi Kataoka : Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip, Clinical Biochemistry, Vol.45, No.15, 1239-1244, 2012.

関連情報

Number of session users = 0, LA = 3.30, Max(EID) = 376460, Max(EOID) = 1008233.