『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=248981EID:248981, Map:0, LastModified:2017年12月4日(月) 13:59:01, Operator:[[ADMIN]], Avail:TRUE, Censor:0, Owner:[苛原 稔], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Abe Akiko (日) (読)
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2.古本 博孝
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[継承]
3.吉田 加奈子 ([徳島大学.病院.中央診療施設等.地域産婦人科診療部])
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4.加藤 剛志 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.産科婦人科学])
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[継承]
5. (英) Saijo Yasuyo (日) (読)
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6.苛原 稔 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.産科婦人科学])
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Gene gun-mediated skin transfection with FL gene suppresses the growth of murine fibrosarcoma.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Particle-mediated transfection is known as an efficient method of non-viral gene transfer. Flt3 ligand (FL) is a growth factor for hematopoietic progenitors; it promotes the growth of dendritic cells (DC). DCs are powerful antigen-presenting cells (APCs) and show a remarkable capacity to stimulate antigen-specific T-cell responses. In this study, we intended to investigate the suppressive effect on tumor growth by gene gun-mediated transfer of FL in a murine model. C57BL/6J mice were injected intradermally with MCA205 cells. DNA (pNGVL-hFLex)-coated gold particles were delivered to the mouse skin surrounding the target tumor. The expression of FL was determined by RT-PCR. Analyses by immunohistochemistry and fluorescence-activated cell sorter (FACS) revealed an increase in the number of DC after treatment with FL. Gene gun-mediated pNGVL-hFLex transfer significantly inhibited the growth of the MCA205 tumor. FL transfer markedly increased the number of CD11c(+) DCs in the tumor tissue. Further, the FL-transfected mice exhibited a significantly higher number of CD80(+) MHC-II cells. We successfully performed FL therapy using an in vivo gene gun in order to effectively mobilize DCs in situ and induce suppressive immunity.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Biolistics (日) (読) [継承]
4. (英) Cell Line, Tumor (日) (読) [継承]
5. (英) Dendritic Cells (日) (読) [継承]
6. (英) Female (日) (読) [継承]
7. (英) Fibrosarcoma (日) (読) [継承]
8.遺伝子発現 (gene expression) [継承]
9.遺伝子治療 (gene therapy) [継承]
10. (英) Membrane Proteins (日) (読) [継承]
11. (英) Mice (日) (読) [継承]
12. (英) Mice, Inbred C57BL (日) (読) [継承]
13. (英) RNA, Messenger (日) (読) [継承]
14. (英) Transfection (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1349-6867
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J. Med. Invest.
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 58 [継承]
(必須): 1-2 [継承]
(必須): 39 45 [継承]
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年月日 (必須): 西暦 2011年 2月 初日 (平成 23年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.2152/jmi.58.39    (→Scopusで検索) [継承]
PMID (任意): 21372485    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-79951938597 [継承]
機関リポジトリ : 83931 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Obstetrics and Gynecology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Akiko Abe, Hiroyuki Furumoto, Kanako Yoshida, Takeshi Katou, Yasuyo Saijo and Minoru Irahara : Gene gun-mediated skin transfection with FL gene suppresses the growth of murine fibrosarcoma., The Journal of Medical Investigation : JMI, Vol.58, No.1-2, 39-45, 2011.
欧文冊子 ● Akiko Abe, Hiroyuki Furumoto, Kanako Yoshida, Takeshi Katou, Yasuyo Saijo and Minoru Irahara : Gene gun-mediated skin transfection with FL gene suppresses the growth of murine fibrosarcoma., The Journal of Medical Investigation : JMI, Vol.58, No.1-2, 39-45, 2011.

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