| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Miura Kouichi (日) (読)
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| 2. | 永井 宏和
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| 3. | (英) Ueno Yasuharu (日) (読)
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| 4. | (英) Goto Takashi (日) (読)
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| 5. | (英) Mikami Ken-ichiro (日) (読)
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| 6. | (英) Nakane Kunio (日) (読)
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| 7. | (英) Yoneyama Kazuo (日) (読)
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| 8. | (英) Watanabe Daisuke (日) (読)
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| 9. | (英) Terada Kunihiko (日) (読)
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| 10. | (英) Sugiyama Toshihiro (日) (読)
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| 11. | (英) Imai Katsuyuki (日) (読)
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| 12. | (英) Senoo Haruki (日) (読)
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| 13. | (英) Watanabe Sumio (日) (読)
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| ○題名 (必須): | □ | (英) Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. (日)
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| ○要約 (任意): | □ | (英) Epimorphin, a mesenchymal cell surface-associated molecule, is detected on hepatic stellate cells (HSCs) in the liver. Here, we show the involvement of epimorphin in differentiation of rat hepatic stem-like cells (HSLCs) through contact with HSCs. HSLCs, isolated from adult rats, cultured in stellate cell-conditioned medium had no phenotypic and morphological changes, whereas HSLCs co-cultured with HSCs expressed albumin, transferrin, and tyrosine aminotransferase. An anti-epimorphin antibody inhibited hepatocytic differentiation of HSLCs in co-culture. Furthermore, epimorphin induced mRNA expression of albumin, transferrin, tyrosine aminotransferase, and gamma-glutamyl transpeptidase with decrease of c-kit and musashi-1. Morphologically, HSLCs piled up when co-cultured with HSCs, which was dramatically inhibited by an anti-epimorphin antibody. HSLCs contact with epimorphin started piling up, changed their shape from flat to cuboidal, and subsequently developed bile-canaliculi-like structures. In conclusion, epimorphin is a factor that induces differentiation of hepatic stem-like cells through epithelial-mesenchymal cell contact. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Cell Communication (日) (読)
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| 3. | 細胞分化 (cell differentiation)
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| 4. | (英) Cell Line (日) (読)
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| 5. | (英) Coculture Techniques (日) (読)
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| 6. | (英) Hepatocytes (日) (読)
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| 7. | (英) Male (日) (読)
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| 8. | (英) Membrane Glycoproteins (日) (読)
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| 9. | (英) Mesenchymal Stem Cells (日) (読)
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| 10. | (英) Rats (日) (読)
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| 11. | (英) Rats, Sprague-Dawley (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)
| ○ISSN (任意): | □ | 0006-291X
ISSN: 0006-291X
(pISSN: 0006-291X, eISSN: 1090-2104) Title: Biochemical and biophysical research communicationsTitle(ISO): Biochem Biophys Res CommunPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login])
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| ○巻 (必須): | □ | 311
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 415 423
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| ○年月日 (必須): | □ | 西暦 2003年 11月 14日 (平成 15年 11月 14日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.bbrc.2003.09.225 (→Scopusで検索)
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| ○PMID (任意): | □ | 14592430 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-10744227518
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| ○備考 (任意): | 1. | (英) Article.Affiliation: First Department of Internal Medicine, Akita University School of Medicine, Akita, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Comparative Study (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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