| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): | □ | Peer Review
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| ○著者 (必須): | 1. | (英) Sakamoto Kozue (日) (読)
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| 2. | 佐藤 陽一 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品情報学])
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| 3. | 新家 利一
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| 4. | 勢井 雅子
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| 5. | 野村 以徂子
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| 6. | 梅野 真由美
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| 7. | エヴィス アシュラフ
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| 8. | 中堀 豊
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| ○題名 (必須): | □ | (英) Proteasome subunits mRNA expressions correlate with male BMI; implications for a role in obesity (日)
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| ○要約 (任意): | □ | (英) Obesity as well as its associated chronic diseases and adverse health consequences such as type 2 diabetes mellitus, dyslipidemia, hypertension, and coronary artery disease are afflicting middle-aged adults and an ever greater number of children globally. We planned to investigate new obesity-related factors using proteomics approaches in a randomly selected three high and three low BMI samples of Epstein-Barr-transformed B (EBV-B) lymphoblastoid cell lines prepared from two groups of young Japanese men with different BMI. To search novel obesity-related factors, comparisons of protein expressions between high and low BMI groups were carried out by two-dimensional gel electrophoresis (2-DE). Gene transcripts of proteasome subunits found out from 2-DE were further determined by quantitative real-time PCR. Results from proteomics approach showed that the expression of proteasome alpha subunit type 5 (PSMA5) was significantly lower in the high BMI male group than in those with low BMI (P < 0.05). To validate these results, we expanded the study to include 20 more men and used real-time PCR to quantify the mRNA expression level in their EBV-B cells. Both PSMA5 and PSMA2 of EBV-B cells showed negative correlation with BMI. Furthermore, the mRNA levels measured in the peripheral blood B lymphocytes for many proteasome subunits in 75 healthy men and women showed significant negative correlation with BMI in healthy men. Our findings suggest that proteasome expression may play a key role in obesity. (日)
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| ○キーワード (推奨): | 1. | (英) Body Mass Index (日) (読)
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| 2. | (英) Cell Line (日) (読)
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| 3. | (英) DNA Primers (日) (読)
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| 4. | (英) Female (日) (読)
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| 5. | (英) Herpesvirus 4, Human (日) (読)
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| 6. | (英) Humans (日) (読)
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| 7. | (英) Male (日) (読)
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| 8. | (英) Obesity (日) (読)
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| 9. | (英) Polymerase Chain Reaction (日) (読)
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| 10. | (英) Proteasome Endopeptidase Complex (日) (読)
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| 11. | (英) Protein Subunits (日) (読)
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| 12. | (英) RNA, Messenger (日) (読)
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| 13. | (英) Reference Values (日) (読)
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| 14. | (英) Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Obesity (The Obesity Society)
(pISSN: 1930-7381, eISSN: 1930-739X)
| ○ISSN (任意): | □ | 1930-7381
ISSN: 1930-7381
(pISSN: 1930-7381, eISSN: 1930-739X) Title: Obesity (Silver Spring, Md.)Title(ISO): Obesity (Silver Spring)Supplier: The Obesity SocietyPublisher: Wiley Publishing (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 17
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| ○号 (必須): | □ | 5
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| ○頁 (必須): | □ | 1044 1049
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2009年 4月 24日 (平成 21年 4月 24日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/oby.2008.612 (→Scopusで検索)
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| ○PMID (任意): | □ | 19165168 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima, Tokushima, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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