『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=188347EID:188347, Map:0, LastModified:2012年9月26日(水) 13:58:03, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[岡村 裕彦], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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学籍番号 (推奨):
[継承]
2.岡村 裕彦
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学籍番号 (推奨):
[継承]
3. (英) Amorim Rabelo Bruna (日) (読)
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[継承]
4.日野出 大輔 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健衛生学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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学籍番号 (推奨):
[継承]
5.吉田 秀夫
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[継承]
6.羽地 達次
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題名 (必須): (英) PKR-mediated degradation of STAT1 regulates osteoblast differentiation  (日)    [継承]
副題 (任意):
要約 (任意): (英) The double-stranded RNA-dependent protein kinase (PKR) plays a critical role in various biological responses including antiviral defense, cell differentiation, apoptosis, and tumorigenesis. In this study, we investigated whether PKR could affect the post-translational modifications of STAT1 protein and whether these modifications regulate osteoblast differentiation. We demonstrated that PKR was necessary for the ubiquitination of STAT1 protein. The expressions of bone-related genes such as type I collagen, integrin binding sialoprotein, osteopontin, and osterix were suppressed in osteoblasts lacking PKR activity. In contrast, the expressions of interleukin-6 and matrix metalloproteinases 8 and 13 increased in PKR-mutated osteoblasts. The expression and degradation of STAT1 protein were regulated by PKR in a SLIM-dependent pathway. Inhibition of SLIM by RNA interference resulted in the decreased activity of Runx2 in osteoblasts. Stimulation of interleukin-6 expression and suppression of alkaline phosphatase activity were regulated through by SLIM-dependent pathway. However, expressions of bone-related genes and MMPs were regulated by SLIM-independent pathway. Our present results suggest that the aberrant accumulation of STAT1 protein induced by loss of PKR regulate osteoblast differentiation through both SLIM/STAT1-dependent and -independent pathways.  (日)    [継承]
キーワード (推奨): 1. (英) Adaptor Proteins, Signal Transducing (日) (読) [継承]
2. (英) Alkaline Phosphatase (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Antigens, Differentiation (日) (読) [継承]
5. (英) Cell Differentiation (日) (読) [継承]
6. (英) Cell Line (日) (読) [継承]
7. (英) Interleukin-6 (日) (読) [継承]
8. (英) LIM Domain Proteins (日) (読) [継承]
9. (英) Metalloproteases (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Osteoblasts (日) (読) [継承]
12. (英) RNA, Messenger (日) (読) [継承]
13. (英) STAT1 Transcription Factor (日) (読) [継承]
14. (英) Ubiquitin-Protein Ligases (日) (読) [継承]
15. (英) Ubiquitination (日) (読) [継承]
16. (英) eIF-2 Kinase (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Experimental Cell Research ([Academic Press])
(pISSN: 0014-4827, eISSN: 1090-2422)

ISSN (任意): 1090-2422
ISSN: 0014-4827 (pISSN: 0014-4827, eISSN: 1090-2422)
Title: Experimental cell research
Title(ISO): Exp. Cell Res.
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 315 [継承]
(必須): 12 [継承]
(必須): 2105 2114 [継承]
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年月日 (必須): 西暦 2009年 7月 初日 (平成 21年 7月 初日) [継承]
URL (任意):
DOI (任意): 10.1016/j.yexcr.2009.02.003    (→Scopusで検索) [継承]
PMID (任意): 19230833    (→Scopusで検索) [継承]
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評価値 (任意): **** [制限]
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備考 (任意): 1.(英) Article.Affiliation: Department of Fundamental Oral Health Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan. kaya@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kaya Yoshida, Hirohiko Okamura, Bruna Rabelo Amorim, Daisuke Hinode, Hideo Yoshida and Tatsuji Haneji : PKR-mediated degradation of STAT1 regulates osteoblast differentiation, Experimental Cell Research, Vol.315, No.12, 2105-2114, 2009.
欧文冊子 ● Kaya Yoshida, Hirohiko Okamura, Bruna Rabelo Amorim, Daisuke Hinode, Hideo Yoshida and Tatsuji Haneji : PKR-mediated degradation of STAT1 regulates osteoblast differentiation, Experimental Cell Research, Vol.315, No.12, 2105-2114, 2009.

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