『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=172052EID:172052, Map:0, LastModified:2018年2月8日(木) 14:03:04, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
3.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔病態制御学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.森本 景之
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2. (英) Ozaki Akiko (日) (読)
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3.岡村 裕彦
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4.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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5.北村 清一郎
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6.羽地 達次
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題名 (必須): (英) Okadaic acid induces tyrosine phosphorylation of IkBa that mediated by PKR pathway in human osteoblastic MG63 cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Treatment of human osteosarcoma cell line MG 63 cells with okadaic acid stimulated phosphorylation of IkappaBalpha, as judged from the results of Western blot analysis and a lambda protein phosphatase dephosphorylation assay. The stimulated phosphorylation of IkappaBalpha was both time- and dose-dependent. The phosphorylation sites of IkappaBalpha were taken to be tyrosine residues because the anti-phospho-tyrosine antibody bound to the samples immunoprecipitated with the anti-IkappaBalpha antibody. In the cells treated with 100 nM okadaic acid consequential translocation of NF-kappaB p65 from the cytosol to the nucleus occurred. Double-stranded RNA-dependent protein kinase (PKR) is a player in the cellular antiviral response and is involved in transcriptional stimulation through activation of NF-kappaB. We investigated the functional relationship between PKR and IkappaBalpha phosphorylation by constructing MG 63 PKR K/R cells that produced a catalytically inactive mutant PKR. NF-kappaB p65 was detected in the nucleus of these cells, even in the unstimulated cells. Although IkappaBalpha was degraded phosphorylation of eIF-2 alpha, a substrate of PKR, did not occur in the mutant cells treated with okadaic acid. Our results suggest that okadaic acid-induced tyrosine phosphorylation of IkappaBalpha was mediated by PKR kinase activity, thus indicating the involvement of this kinase in the control mechanism governing the activation of NF-kappaB.  (日)    [継承]
キーワード (推奨): 1. (英) Cell Line, Tumor (日) (読) [継承]
2. (英) Humans (日) (読) [継承]
3. (英) I-kappa B Proteins (日) (読) [継承]
4. (英) Okadaic Acid (日) (読) [継承]
5. (英) Osteoblasts (日) (読) [継承]
6. (英) Osteosarcoma (日) (読) [継承]
7. (英) Phosphorylation (日) (読) [継承]
8. (英) Phosphotyrosine (日) (読) [継承]
9. (英) eIF-2 Kinase (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular and Cellular Biochemistry (Springer)
(pISSN: 0300-8177, eISSN: 1573-4919)

ISSN (任意): 0300-8177
ISSN: 0300-8177 (pISSN: 0300-8177, eISSN: 1573-4919)
Title: Molecular and cellular biochemistry
Title(ISO): Mol Cell Biochem
Supplier: Kluwer Online
Publisher: Springer US
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 276 [継承]
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年月日 (必須): 西暦 2005年 8月 初日 (平成 17年 8月 初日) [継承]
URL (任意):
DOI (任意): 10.1007/s11010-005-4440-y    (→Scopusで検索) [継承]
PMID (任意): 16132703    (→Scopusで検索) [継承]
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WOS (任意): 000231220500026 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan. morimoto@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hiroyuki Morimoto, Akiko Ozaki, Hirohiko Okamura, Kaya Yoshida, Seiichiro Kitamura and Tatsuji Haneji : Okadaic acid induces tyrosine phosphorylation of IkBa that mediated by PKR pathway in human osteoblastic MG63 cells, Molecular and Cellular Biochemistry, Vol.276, No.1-2, 211-217, 2005.
欧文冊子 ● Hiroyuki Morimoto, Akiko Ozaki, Hirohiko Okamura, Kaya Yoshida, Seiichiro Kitamura and Tatsuji Haneji : Okadaic acid induces tyrosine phosphorylation of IkBa that mediated by PKR pathway in human osteoblastic MG63 cells, Molecular and Cellular Biochemistry, Vol.276, No.1-2, 211-217, 2005.

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