『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
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組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.創薬資源科学部門.機能分子創製学講座 (2004年4月1日〜) [継承]
著者 (必須): 1. (英) Tadatsu Yoko (日) (読)
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2.六車 直樹 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学]/[徳島大学.病院.診療科.内科.消化器内科])
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3.伊東 進
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[継承]
4. (英) Tadatsu Masaya (日) (読)
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[継承]
5. (英) Kusaka Yoshihiro (日) (読)
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6.岡本 耕一 ([徳島大学.病院.診療科.内科.消化器内科])
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[継承]
7. (英) Imoto Yoshitaka (日) (読)
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[継承]
8. (英) Taue Hiromi (日) (読)
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[継承]
9.佐野 茂樹 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.分子創薬化学])
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10.長尾 善光
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[継承]
題名 (必須): (英) Optimal Labeling Condition of Antibodies Available for Immunofluorescence Endoscopy  (日)    [継承]
副題 (任意):
要約 (任意): (英) In recent years, labeled antibodies have been used for diagnostic imaging in many studies. In this study, we investigated the mode of binding in antibodies labeled with ICG derivatives newly developed for the diagnosis of microcarcinomas, and evaluated the optimal binding molar ratio between the labeling compounds and antibody. MUC 1 antibody and ICG derivatives (ICG-ATT and ICG-sulfo-OSu) were used. ICG derivatives non-covalently bound to the antibody were removed with ethyl acetate, and the ratio of ICG derivatives covalently bound to the labeled antibody was confirmed. During purification of the labeled antibody, the amount of each labeling compound reacting with 1 molecule of the antibody varied as follows: 4, 8, 16, and 32 molar equivalents. Subsequently, the intensity of fluorescence was evaluated by spectroscopy and infrared fluoroscopy. The ratio of residual ICG derivative labeling the antibody was 67.4% for ICG-ATT and 65.0% for ICG-sulfo-OSu. When fluorescent antibody labeled with ICG-ATT at an F/P ratio of 2.94 or 4.18 was used, specific and clear fluorescent images of the antigen were obtained. When ICG-ATT-labeled antibody at an F/P ratio of 6.50 or 6.75 was used, the fluorescence intensity decreased and the fluorescent images of antigen became unclear. It was found that the ICG-ATT-labeled antibody was a more specific and sensitive marker than ICG-sulfo-OSu-labeled antibody, and that lower binding molar ratios of ICG-ATT were more useful for labeling the antibody.  (日)    [継承]
キーワード (推奨): 1. (英) Antibodies, Neoplasm (日) (読) [継承]
2. (英) Antigens, Neoplasm (日) (読) [継承]
3. (英) Endoscopy (日) (読) [継承]
4. (英) Fluorescent Antibody Technique (日) (読) [継承]
5. (英) Fluorescent Dyes (日) (読) [継承]
6. (英) Gastroscopy (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Indocyanine Green (日) (読) [継承]
9. (英) Infrared Rays (日) (読) [継承]
10. (英) Mucin-1 (日) (読) [継承]
11. (英) Mucins (日) (読) [継承]
12. (英) Stomach Neoplasms (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1343-1420
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J Med Invest
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 53 [継承]
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(必須): 52 60 [継承]
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年月日 (必須): 西暦 2006年 2月 初日 (平成 18年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.2152/jmi.53.52    (→Scopusで検索) [継承]
PMID (任意): 16537996    (→Scopusで検索) [継承]
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機関リポジトリ : 110788 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Yoko Tadatsu, Naoki Muguruma, Susumu Ito, Masaya Tadatsu, Yoshihiro Kusaka, Koichi Okamoto, Yoshitaka Imoto, Hiromi Taue, Shigeki Sano and Yoshimitsu Nagao : Optimal Labeling Condition of Antibodies Available for Immunofluorescence Endoscopy, The Journal of Medical Investigation : JMI, Vol.53, No.1/2, 52-60, 2006.
欧文冊子 ● Yoko Tadatsu, Naoki Muguruma, Susumu Ito, Masaya Tadatsu, Yoshihiro Kusaka, Koichi Okamoto, Yoshitaka Imoto, Hiromi Taue, Shigeki Sano and Yoshimitsu Nagao : Optimal Labeling Condition of Antibodies Available for Immunofluorescence Endoscopy, The Journal of Medical Investigation : JMI, Vol.53, No.1/2, 52-60, 2006.

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