『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.東海大学 [継承]
2.徳島大学.大学院ヘルスバイオサイエンス研究部.先端基礎医学教育研究プロジェクト (2005年1月1日〜2015年3月31日) [継承]
著者 (必須): 1. (英) Ohtani Yasuo (日) (読)
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2.日吉 峰麗
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3. (英) Ohkubo Tomoichi (日) (読)
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4. (英) Tsuji Kimiyoshi (日) (読)
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5. (英) Hagihara Masao (日) (読)
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6. (英) Nakasaki Hisao (日) (読)
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7. (英) Makuuchi Hiroyasu (日) (読)
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8. (英) Nagata Naruhiko (日) (読)
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9. (英) Mine Tetsuya (日) (読)
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10. (英) Takada Shigeo (日) (読)
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11. (英) Yamamura Masaichi (日) (読)
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12. (英) Tsuda Michio (日) (読)
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題名 (必須): (英) A liver-derived immunosuppressive factor is an arginase: identification and mechanism of immunosuppression  (日)    [継承]
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要約 (任意): (英) We found a substance in culture medium of neonatal pig liver fragments, which suppresses an immune response monitored by (3)H-thymidine incorporation using phytohemagglutinin (PHA)-stimulated lymphocytes. We named it as an immunosuppressive factor (ISF). To purify ISF, ammonium sulfate fractionation, DE52, SP-Sephadex, hydroxyapatite, blue Sepharose, heparin Sepharose and Superdex gel filtration columns were used. Using these purification procedures, ISF was purified 1,254-fold, with 9.2% recovery, from the culture medium of neonatal pig liver fragments, and was identified as arginase by its biochemical characteristics including molecular size, amino acid sequences of digested peptides and expression of arginase activity. The addition of ISF caused to decrease in arginine concentration in culture medium and at the same time DNA synthesis was suppressed dose-dependently, both of which were recovered by the addition of NOHA (N(G)-hydroxy-L-arginine), an arginase inhibitor. In addition, the depletion of arginine in culture medium also led to the inhibition of DNA synthesis. These results led us to the conclusion that immunosuppressive effect of ISF was due to arginase activity that decreased arginine concentration in culture medium, not to another function of ISF.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Animals, Newborn (日) (読) [継承]
4. (英) Arginase (日) (読) [継承]
5. (英) Arginine (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7. (英) Immune Tolerance (日) (読) [継承]
8. (英) Liver (日) (読) [継承]
9. (英) Molecular Sequence Data (日) (読) [継承]
10. (英) Organ Culture Techniques (日) (読) [継承]
11. (英) Swine (日) (読) [継承]
発行所 (推奨): (英) Biomedical Research Press (日) (読) [継承]
誌名 (必須): Biomedical Research (Biomedical Research Foundation (Japan))
(pISSN: 0388-6107, eISSN: 1880-313X)

ISSN (任意): 0388-6107
ISSN: 0388-6107 (pISSN: 0388-6107, eISSN: 1880-313X)
Title: Biomedical research (Tokyo, Japan)
Title(ISO): Biomed Res
Supplier: バイオメディカルリサーチプレス
Publisher: Biomedical Research Foundation
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2007年 2月 初日 (平成 19年 2月 初日) [継承]
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DOI (任意): 10.2220/biomedres.28.17    (→Scopusで検索) [継承]
PMID (任意): 17379953    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Surgical Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Yasuo Ohtani, Mineyoshi Hiyoshi, Tomoichi Ohkubo, Kimiyoshi Tsuji, Masao Hagihara, Hisao Nakasaki, Hiroyasu Makuuchi, Naruhiko Nagata, Tetsuya Mine, Shigeo Takada, Masaichi Yamamura and Michio Tsuda : A liver-derived immunosuppressive factor is an arginase: identification and mechanism of immunosuppression, Biomedical Research, Vol.28, No.1, 17-23, 2007.
欧文冊子 ● Yasuo Ohtani, Mineyoshi Hiyoshi, Tomoichi Ohkubo, Kimiyoshi Tsuji, Masao Hagihara, Hisao Nakasaki, Hiroyasu Makuuchi, Naruhiko Nagata, Tetsuya Mine, Shigeo Takada, Masaichi Yamamura and Michio Tsuda : A liver-derived immunosuppressive factor is an arginase: identification and mechanism of immunosuppression, Biomedical Research, Vol.28, No.1, 17-23, 2007.

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