『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=146488EID:146488, Map:0, LastModified:2017年12月4日(月) 14:07:00, Operator:[[ADMIN]], Avail:TRUE, Censor:承認済, Owner:[髙橋 章], Read:継承, Write:継承, Delete:継承.
○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨):
○カテゴリ (推奨):
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨):
○著者 (必須): 1. (英) Miki Mari (日) (読)
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○学籍番号 (推奨):
[継承]
2. (英) Nakamura Yoichi (日) (読)
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[継承]
3.髙橋 章 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.予防環境栄養学])
○役割 (任意):
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○学籍番号 (推奨):
[継承]
4.中屋 豊
○役割 (任意):
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○学籍番号 (推奨):
[継承]
5. (英) Eguchi Hiroshi (日) (読)
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○学籍番号 (推奨):
[継承]
6. (英) Masegi Tsukio (日) (読)
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○学籍番号 (推奨):
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7. (英) Yoneda Kazuo (日) (読)
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8.安岡 劭
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○学籍番号 (推奨):
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9.曽根 三郎
○役割 (任意):
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○学籍番号 (推奨):
[継承]
○題名 (必須): □(英) Effect of human airway trypsin-like protease on intracellular free Ca2+ concentration in human bronchial epithelial cells  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) It has been shown that human airway trypsin-like protease (HAT) is localized in human bronchial epithelial cells (HBEC), and trypsin activates protease-activated receptor-2 (PAR-2). Activation of PAR-2 activates G-protein followed by an increase of intracellular free Ca2+, [Ca2+]in. This study was undertaken to clarify whether HAT can activate PAR-2 in HBEC or not. RT-PCR showed that HAT mRNA is expressed in HBEC, and PAR-2 mRNA is the most strongly expressed of the known PARs in HBEC. Both PAR-2 agonist peptide (PAR-2 AP) and HAT increased [Ca2+]in in HBEC in a biphasic fashion; a prompt, sharp increase (peak I) and a sustained low plateau (peak II). PAR-2 AP over 100-200 microM and HAT over 200-300 mU/ml (0.08-0.12 microM) induced both peak I and II, and PAR-2 AP below 100 microM and HAT below 200 mU/ml induced only peak II. Both PAR-2 AP-induced and HAT-induced peak I were induced by Ca2+ mobilization from intracellular stores, because they appeared even in Ca2+-free medium. Both PAR-2 AP-induced and HAT-induced peak II were induced by an influx of extracellular Ca2+, because they were abolished in Ca2+-free medium. The Ca2+ response to HAT was desensitized by exposure of HBEC to PAR-2 AP. These results indicate that HBEC have a functional PAR-2, and HAT regulates cellular functions of HBEC via activation of PAR-2.  (日)    [継承]
○キーワード (推奨): 1. (英) Adult (日) (読) [継承]
2. (英) Bronchi (日) (読) [継承]
3. (英) Calcium (日) (読) [継承]
4. (英) Calcium Signaling (日) (読) [継承]
5. (英) Cell Line, Transformed (日) (読) [継承]
6. (英) Cells, Cultured (日) (読) [継承]
7. (英) Culture Media (日) (読) [継承]
8. (英) Enzyme Induction (日) (読) [継承]
9. (英) Epithelial Cells (日) (読) [継承]
10. (英) Fibroblasts (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Intracellular Fluid (日) (読) [継承]
13. (英) Ion Transport (日) (読) [継承]
14. (英) Lung (日) (読) [継承]
15. (英) Macrophages, Alveolar (日) (読) [継承]
16. (英) Peptide Fragments (日) (読) [継承]
17. (英) RNA, Messenger (日) (読) [継承]
18. (英) Receptor, PAR-1 (日) (読) [継承]
19. (英) Receptor, PAR-2 (日) (読) [継承]
20. (英) Receptors, Thrombin (日) (読) [継承]
21. (英) Recombinant Fusion Proteins (日) (読) [継承]
22. (英) Serine Endopeptidases (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

○ISSN (任意): □1343-1420
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J Med Invest
Supplier: 徳島大学
Publisher: University of Tokushima
 (NLM Catalog)   (CiNii NCID)   (医中誌Web)   (J-STAGE)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □50 [継承]
○号 (必須): □1-2 [継承]
○頁 (必須): □95 107 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2003年 2月 初日 (平成 15年 2月 初日) [継承]
○URL (任意): □http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12630574&dopt=Abstract [継承]
○DOI (任意):
○PMID (任意): □12630574    (→Scopusで検索) [継承]
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○Scopus (任意):
○機関リポジトリ : □110683 [継承]
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○備考 (任意): 1.(英)   (日) 著者5. 確認済 修正不要   [継承]

標準的な表示

和文冊子 ● Mari Miki, Yoichi Nakamura, Akira Takahashi, Yutaka Nakaya, Hiroshi Eguchi, Tsukio Masegi, Kazuo Yoneda, Susumu Yasuoka and Saburo Sone : Effect of human airway trypsin-like protease on intracellular free Ca2+ concentration in human bronchial epithelial cells, The Journal of Medical Investigation : JMI, 50, 1-2, 95-107, 2003.
欧文冊子 ● Mari Miki, Yoichi Nakamura, Akira Takahashi, Yutaka Nakaya, Hiroshi Eguchi, Tsukio Masegi, Kazuo Yoneda, Susumu Yasuoka and Saburo Sone : Effect of human airway trypsin-like protease on intracellular free Ca2+ concentration in human bronchial epithelial cells, The Journal of Medical Investigation : JMI, 50, 1-2, 95-107, 2003.

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