『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=128063)

EID=128063EID:128063, Map:0, LastModified:2017年12月4日(月) 14:07:36, Operator:[[ADMIN]], Avail:TRUE, Censor:0, Owner:[片岡 佳子], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Miki Tsuyoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.桑原 知巳
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3.中山 治之
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Okada Natsumi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.片岡 佳子 ([徳島大学.大学院医歯薬学研究部.保健科学域.保健科学部門.医用検査学系.微生物・遺伝子解析学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6.有持 秀喜 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Ohnishi Yoshinari (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Simultaneous detection of Bacteroides fragilis group species by leuB-directed PCR  (日)    [継承]
副題 (任意):
要約 (任意): (英) Bacteroides species, saccharolytic Gram-negative obligate anaerobes, are frequently isolated from human infections such as peritonitis, abscesses and bacteremia. Among the species in the genus Bacteroides, the species called "B. fragilis group" are particularly involved in human infections and are medically important because they account for a major part of anaerobic isolates from clinical specimens. The purpose of this study was to develop PCR primers that specifically and simultaneously amplify the beta -isopropylmalate dehydrogenase gene leuB in B. fragilis group species. We determined partial nucleotide sequences of leuB genes and compared them in seventeen strains of nine B. fragilis group species, and the regions that are conserved among Bacteroides strains but different from other species were used as a B. fragilis group-specific PCR primer set, BacLBF-BacLBR. Specificity tests of the primer set using 52 phenotypically characterized strains and 75 isolates from rat feces showed only one case each of false-positive and false-negative. The detection limit of the leuB-directed PCR using BacLBF and BacLBR was 3.9 x 10(3) colony-forming units. These results indicate that leuB amplification using BacLBF andBacLBR is a useful tool for rapid diagnosis of Bacteriodes infection and for rapid differential diagnosis of anaerobic infections.  (日)    [継承]
キーワード (推奨): 1. (英) Bacteroides (日) (読) [継承]
2. (英) leuB (日) (読) [継承]
3. (英) PCR (日) (読) [継承]
4. (英) rapid detection (日) (読) [継承]
発行所 (推奨): 徳島大学.医学部 [継承]
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1343-1420
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J. Med. Invest.
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 52 [継承]
(必須): 1,2 [継承]
(必須): 101 108 [継承]
都市 (任意):
年月日 (必須): 西暦 2005年 2月 初日 (平成 17年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.2152/jmi.52.101    (→Scopusで検索) [継承]
PMID (任意): 15751280    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
機関リポジトリ : 110763 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意):

標準的な表示

和文冊子 ● Tsuyoshi Miki, Tomomi Kuwahara, Haruyuki Nakayama, Natsumi Okada, Keiko Kataoka, Hideki Arimochi and Yoshinari Ohnishi : Simultaneous detection of Bacteroides fragilis group species by leuB-directed PCR, The Journal of Medical Investigation : JMI, Vol.52, No.1,2, 101-108, 2005.
欧文冊子 ● Tsuyoshi Miki, Tomomi Kuwahara, Haruyuki Nakayama, Natsumi Okada, Keiko Kataoka, Hideki Arimochi and Yoshinari Ohnishi : Simultaneous detection of Bacteroides fragilis group species by leuB-directed PCR, The Journal of Medical Investigation : JMI, Vol.52, No.1,2, 101-108, 2005.

関連情報

Number of session users = 0, LA = 1.11, Max(EID) = 360769, Max(EOID) = 966336.