『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=101464)

EID=101464EID:101464, Map:0, LastModified:2014年4月30日(水) 14:10:15, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[安友 康二], Read:継承, Write:継承, Delete:継承.
○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨): □Peer Review [継承]
○カテゴリ (推奨):
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨):
○著者 (必須): 1.安友 康二 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])
○役割 (任意):
○貢献度 (任意):
○学籍番号 (推奨):
[継承]
2. (英) Lucas Bruno (日) (読)
○役割 (任意):
○貢献度 (任意):
○学籍番号 (推奨):
[継承]
3. (英) Germain N Ronald (日) (読)
○役割 (任意):
○貢献度 (任意):
○学籍番号 (推奨):
[継承]
○題名 (必須): □(英) TCR signaling for initiation and completion of thymocyte positive selection has distinct requirements for ligand quality and presenting cell type  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) Thymocyte selection involves signaling by TCR engaging diverse self-peptide:MHC molecule ligands on various cell types in the cortex and medulla. Here we separately analyze early and late stages of selection to better understand how presenting cell type, ligand quality, and the timing of TCR signaling contribute to intrathymic differentiation. TCR transgenic CD4+CD8+ thymocytes (double positive (DP)) from MHC-deficient mice were stimulated using various presenting cells and ligands. The resulting CD69high cells were isolated and evaluated for maturation in reaggregate cultures with wild-type or MHC molecule-deficient thymic stroma with or without added hemopoietic dendritic cells (DC). Production of CD4+ T cells required TCR signaling in the reaggregates, indicating that transient recognition of self-ligands by DP is inadequate for full differentiation. DC bearing a potent agonist ligand could initiate positive selection, producing activated thymocytes that matured into agonist-responsive T cells in reaggregates lacking the same ligand. DC could also support the TCR signaling necessary for late maturation. These results argue that despite the negative role assigned to DC in past studies, neither the peptide:MHC molecule complexes present on DC nor any other signals provided by these cells stimulate only thymocyte death. These findings also indicate that unique epithelial ligands are not necessary for positive selection. They provide additional insight into the role of ligand quality in selection events and support the concept that following initiation of maturation from the DP state, persistent TCR signaling is characteristic of and perhaps required by T cells.  (日)    [継承]
○キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Antigen-Presenting Cells (日) (読) [継承]
3. (英) Antigens, CD (日) (読) [継承]
4. (英) Antigens, CD4 (日) (読) [継承]
5. (英) Antigens, CD8 (日) (読) [継承]
6. (英) Antigens, Differentiation, T-Lymphocyte (日) (読) [継承]
7. (英) CD4-Positive T-Lymphocytes (日) (読) [継承]
8. (英) Cell Aggregation (日) (読) [継承]
9. (英) Cell Differentiation (日) (読) [継承]
10. (英) Cell Survival (日) (読) [継承]
11. (英) Cells, Cultured (日) (読) [継承]
12. (英) Cytochrome c Group (日) (読) [継承]
13. (英) Dendritic Cells (日) (読) [継承]
14. (英) Histocompatibility Antigens Class II (日) (読) [継承]
15. (英) Immunophenotyping (日) (読) [継承]
16. (英) Lectins, C-Type (日) (読) [継承]
17. (英) Ligands (日) (読) [継承]
18. (英) Lymphocyte Activation (日) (読) [継承]
19. (英) Major Histocompatibility Complex (日) (読) [継承]
20. (英) Mice (日) (読) [継承]
21. (英) Mice, Inbred C57BL (日) (読) [継承]
22. (英) Mice, Knockout (日) (読) [継承]
23. (英) Mice, Transgenic (日) (読) [継承]
24. (英) Peptides (日) (読) [継承]
25. (英) Proto-Oncogene Proteins c-bcl-2 (日) (読) [継承]
26. (英) Receptors, Antigen, T-Cell (日) (読) [継承]
27. (英) Signal Transduction (日) (読) [継承]
28. (英) Stromal Cells (日) (読) [継承]
29. (英) T-Lymphocyte Subsets (日) (読) [継承]
30. (英) Thymus Gland (日) (読) [継承]
31. (英) Up-Regulation (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

○ISSN (任意): □0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □165 [継承]
○号 (必須): □6 [継承]
○頁 (必須): □3015 3022 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2000年 9月 15日 (平成 12年 9月 15日) [継承]
○URL (任意): □http://jimmunol.org/cgi/content/abstract/165/6/3015 [継承]
○DOI (任意):
○PMID (任意): □10975810    (→Scopusで検索) [継承]
○CRID (任意):
○WOS (任意): □000165938100014 [継承]
○Scopus (任意): □2-s2.0-0034665493 [継承]
○評価値 (任意):
○被引用数 (任意):
○指導教員 (推奨):
○備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Koji Yasutomo, Bruno Lucas and Ronald N Germain : TCR signaling for initiation and completion of thymocyte positive selection has distinct requirements for ligand quality and presenting cell type, The Journal of Immunology, Vol.165, No.6, 3015-3022, 2000.
欧文冊子 ● Koji Yasutomo, Bruno Lucas and Ronald N Germain : TCR signaling for initiation and completion of thymocyte positive selection has distinct requirements for ligand quality and presenting cell type, The Journal of Immunology, Vol.165, No.6, 3015-3022, 2000.

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