『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Nakashiro Koichi (日) (読)
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[継承]
2. (英) Kawamata Hitoshi (日) (読)
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3. (英) Hino Satoshi (日) (読)
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4.内田 大亮
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5. (英) Miwa Yoshihiro (日) (読)
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6. (英) Hamano Hironori (日) (読)
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7.表原 文江
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8.吉田 秀夫
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9.佐藤 光信
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題名 (必須): (英) Down-regulation of TSC-22 (transforming growth factor beta-stimulated clone 22) markedly enhances the growth of a human salivary gland cancer cell line in vitro and in vivo  (日)    [継承]
副題 (任意):
要約 (任意): (英) We have recently isolated TSC-22 (transforming growth factor beta-stimulated clone 22) cDNA as a new anticancer drug (Vesnarinone)-inducible gene in a human salivary gland cancer cell line, TYS. We conducted the present study to examine whether up-regulation or down-regulation of TSC-22 can affect the growth of TYS cells in vitro and in vivo. We constructed an expression vector containing sense- or antisense-oriented human TSC-22 cDNA under the transcriptional control of the SR alpha promoter. We cotransfected TYS cells with the sense or antisense expression vector and pSV2neo and obtained more than 200 G418-resistant colonies in each sense or antisense transfectant. Approximately 80% of representative G418-resistant clones expressed the transcripts from transfected sense or antisense TSC-22 cDNA. To avoid the clonal heterogeneity of the cells, we mixed all of the G418-resistant colonies together in each sense or antisense transfectant and examined the expression of TSC-22 protein, in vitro growth, and the tumorigenicity in nude mice. The expression of TSC-22 protein was examined by solid-phase ELISA using a specific antibody against recombinant TSC-22 protein. The expression of TSC-22 protein was up-regulated in the sense transfectants and down-regulated in the antisense transfectants. Contrary to our expectation, up-regulation of TSC-22 protein did not affect both in vitro and in vivo growth of TYS cells. However, down-regulation of TSC-22 markedly enhanced the growth of TYS cells in vitro and in vivo. Furthermore, we examined the expression of TSC-22 mRNA in several human salivary gland tumors. The mRNA expression of TSC-22 in benign and malignant salivary gland tumors was significantly decreased when compared to that in tumor-free salivary glands (P < 0.05; one-way ANOVA), and in some salivary gland tumors, the expression of TSC-22 mRNA was not detectable by reverse transcription-PCR. These results suggest that down-regulation of TSC-22 may play a major role on salivary gland tumorigenesis.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Antineoplastic Agents (日) (読) [継承]
3. (英) Cell Transformation, Neoplastic (日) (読) [継承]
4. (英) Clone Cells (日) (読) [継承]
5. (英) DNA, Antisense (日) (読) [継承]
6. (英) DNA, Complementary (日) (読) [継承]
7. (英) Gene Expression Regulation, Neoplastic (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Leucine Zippers (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Mice, Nude (日) (読) [継承]
12. (英) Neoplasm Proteins (日) (読) [継承]
13. (英) Neoplasm Transplantation (日) (読) [継承]
14. (英) Promoter Regions, Genetic (日) (読) [継承]
15. (英) Quinolines (日) (読) [継承]
16. (英) RNA, Messenger (日) (読) [継承]
17. (英) RNA, Neoplasm (日) (読) [継承]
18. (英) Recombinant Fusion Proteins (日) (読) [継承]
19. (英) Repressor Proteins (日) (読) [継承]
20. (英) Salivary Gland Neoplasms (日) (読) [継承]
21. (英) Transcription Factors (日) (読) [継承]
22. (英) Transcription, Genetic (日) (読) [継承]
23. (英) Transfection (日) (読) [継承]
24. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Cancer Research (American Assotiation for Cancer Research)
(pISSN: 0008-5472, eISSN: 1538-7445)

ISSN (任意): 0008-5472
ISSN: 0008-5472 (pISSN: 0008-5472, eISSN: 1538-7445)
Title: Cancer research
Title(ISO): Cancer Res.
Publisher: American Association for Cancer Research
 (NLM Catalog  (Scopus  (Scopus  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 58 [継承]
(必須): 3 [継承]
(必須): 549 555 [継承]
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年月日 (必須): 西暦 1998年 2月 1日 (平成 10年 2月 1日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 9458104    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000071710700032 [継承]
Scopus (任意): 2-s2.0-0032005230 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Koichi Nakashiro, Hitoshi Kawamata, Satoshi Hino, Daisuke Uchida, Yoshihiro Miwa, Hironori Hamano, Fumie Omotehara, Hideo Yoshida and Mitsunobu Sato : Down-regulation of TSC-22 (transforming growth factor beta-stimulated clone 22) markedly enhances the growth of a human salivary gland cancer cell line in vitro and in vivo, Cancer Research, Vol.58, No.3, 549-555, 1998.
欧文冊子 ● Koichi Nakashiro, Hitoshi Kawamata, Satoshi Hino, Daisuke Uchida, Yoshihiro Miwa, Hironori Hamano, Fumie Omotehara, Hideo Yoshida and Mitsunobu Sato : Down-regulation of TSC-22 (transforming growth factor beta-stimulated clone 22) markedly enhances the growth of a human salivary gland cancer cell line in vitro and in vivo, Cancer Research, Vol.58, No.3, 549-555, 1998.

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