『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=98192)

EID=98192EID:98192, Map:0, LastModified:2012年12月20日(木) 11:04:10, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[細井 和雄], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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共著種別 (推奨):
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組織 (推奨):
著者 (必須): 1. (英) Fogaça,E. Sandro (日) (読)
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2. (英) Melo,L. Robson (日) (読)
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3. (英) Pimenta,C. Daniel (日) (読)
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4.細井 和雄
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5. (英) Juliano,Luiz (日) (読)
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6. (英) Juliano,A. Maria (日) (読)
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題名 (必須): (英) Difference in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins  (日)    [継承]
副題 (任意):
要約 (任意): (英) The kininogenase activities of mouse (mK1), rat (rK1) and human (hK1) tissue kallikreins were assayed with the bradykinin-containing synthetic peptides Abz-MTEMARRPPGFSPFRSVTVQNH2 (where Abz stands for o-aminobenzoyl) and Abz-MTSVIRRPPGFSPFRAPRV-NH2, which correspond to fragments Met374-Gln393 and Met375-Val393 of mouse and rat LMWKs (low-molecular-mass kininogens) with the addition of Abz. Bradykinin was released from these peptides by the mK1- and rK1-mediated hydrolysis of Arg-Arg and Arg-Ser (or Arg-Ala) peptide bonds. However, owing to preferential hydrolysis of Phe-Arg compared with the Arg-Ala bond in the peptide derived from rat LMWK, hK1 released bradykinin only from the mouse LMWK fragment and preferentially released des-[Arg9]bradykinin from the rat LMWK fragment (Abz-MTSVIRRPPGFSPFRAPRV-NH2). The formation of these hydrolysis products was examined in more detail by determining the kinetic parameters for the hydrolysis of synthetic, internally quenched fluorescent peptides containing six N- or C-terminal amino acids of bradykinin added to the five downstream or upstream residues of mouse and rat kininogens respectively. One of these peptides, Abz-GFSPFRAPRVQ-EDDnp (where EDDnp stands for ethylenediamine 2,4-dinitrophenyl), was preferentially hydrolysed at the Phe-Arg bond, confirming the potential des-[Arg9]bradykinin-releasing activity of hK1 on rat kininogen. The proline residue that is two residues upstream of bradykinin in rat kininogen is, in part, responsible for this pattern of hydrolysis, since the peptide Abz-GFSPFRASRVQ-EDDnp was preferentially cleaved at the Arg-Ala bond by hK1. Since this peptidase accepts the arginine or phenylalanine residue at its S1 subsite, this preference seems to be determined by the prime site of the substrates. These findings also suggested that the effects observed in rats overexpressing hK1 should consider the activation of B1 receptors by des-[Arg9]bradykinin. For further comparison, two short internally quenched fluorescent peptides that bind to hK1 with affinity in the nM range and some inhibitors described previously for hK1 were also assayed with mK1 and rK1.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Bradykinin (日) (読) [継承]
4. (英) Enzyme Inhibitors (日) (読) [継承]
5. (英) Female (日) (読) [継承]
6. (英) Fluorescent Dyes (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Hydrolysis (日) (読) [継承]
9. (英) Kinins (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Mice, Inbred ICR (日) (読) [継承]
12. (英) Molecular Sequence Data (日) (読) [継承]
13. (英) Peptide Fragments (日) (読) [継承]
14. (英) Peptides (日) (読) [継承]
15. (英) Rats (日) (読) [継承]
16. (英) Substrate Specificity (日) (読) [継承]
17. (英) Tissue Kallikreins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 1470-8728
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 380 [継承]
(必須): Pt3 [継承]
(必須): 775 781 [継承]
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年月日 (必須): 西暦 2004年 6月 15日 (平成 16年 6月 15日) [継承]
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DOI (任意): 10.1042/BJ20031047    (→Scopusで検索) [継承]
PMID (任意): 15040788    (→Scopusで検索) [継承]
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WOS (任意): 000222338100021 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Tres de Maio 100, São Paulo 04044-20, Brazil.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC1224235  (日)    [継承]

標準的な表示

和文冊子 ● Sandro E. Fogaça, Robson L. Melo, Daniel C. Pimenta, Kazuo Hosoi, Luiz Juliano and Maria A. Juliano : Difference in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins, The Biochemical Journal, Vol.380, No.Pt3, 775-781, 2004.
欧文冊子 ● Sandro E. Fogaça, Robson L. Melo, Daniel C. Pimenta, Kazuo Hosoi, Luiz Juliano and Maria A. Juliano : Difference in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins, The Biochemical Journal, Vol.380, No.Pt3, 775-781, 2004.

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