『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨):
○カテゴリ (推奨):
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨): 1.大阪大学.医学研究科 [継承]
2.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔再建医学講座 (2004年4月1日〜) [継承]
○著者 (必須): 1. (英) Higashibata Yuji (日) (読)
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[継承]
2. (英) Sakuma Takahiko (日) (読)
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[継承]
3. (英) Kawahata Hirohisa (日) (読)
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[継承]
4.藤原 慎視
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[継承]
5.森山 啓司
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○学籍番号 (推奨):
[継承]
6. (英) Okada Atsushi (日) (読)
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[継承]
7. (英) Yasui Takahiro (日) (読)
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8. (英) Kohri Kenjiro (日) (読)
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9. (英) Kitamura Yukihiko (日) (読)
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○学籍番号 (推奨):
[継承]
10. (英) Nomura Shintaro (日) (読)
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○学籍番号 (推奨):
[継承]
○題名 (必須): □(英) Identification of Promoter Regions Involved in Cell- and Developmental Stage-Specific Osteopontin Expression in Bone, Kidney, Placenta, and Mammary Gland: An Analysis of Transgenic Mice  (日)    [継承]
○副題 (任意): □(英) An analysis of transgenic mice.  (日)    [継承]
○要約 (任意): □(英) Cell-specific expression of GFP under the control of different lengths of the osteopontin promoter in transgenic mice identified the positive and negative regulatory regions for respective cell types. The results provide new insights for physiological and pathological expression of the osteopontin gene. Osteopontin (OPN) is a major non-collagenous bone matrix protein that is involved in normal and pathological calcification and is expressed in a tissue-specific manner. To investigate how such tissue-specific OPN gene expression is regulated in vivo, transgenic mice expressing the green fluorescent protein (GFP) reporter gene controlled by different lengths of the OPN promoter were generated. Cell- and developmental stage-specific osteopontin expression in transgenic mice was examined by Northern blotting, immunoblotting, fluorescence examination, and in situ hybridization and compared with those of OPN. The line bearing the -5505 to +14 region of the OPN promoter was shown by Northern blotting and immunoblotting to express GFP in the same cells that express endogenous OPN (osteoblasts, hypertrophic chondrocytes, renal and mammary gland epithelial cells, and granulated metrial gland [GMG] placental cells) at the same stage in development. Thus, the 5.5-kb -5505 to +14 promoter region is sufficient for proper tissue-specific OPN expression. The lines carrying shorter segments of the OPN promoter showed different expression patterns. These patterns revealed a putative cis-acting element in the -5269 to -5263 region that restricts OPN expression to hypertrophic chondrocytes and a mammary gland-specific expressing element and a GMG cell-specific enhancing element in the -5505 to -3156 region. Furthermore, the -3155 to -1576 region seems to contain positive renal epithelial cell- and GMG cell-specific expression motif(s) as well as a negative regulatory element that prevents OPN expression in fibroblasts. Moreover, the -1576 to -910 region seems to contain a positive osteoblast-specific-expressing element. Thus, the 5.5-kb OPN promoter contains multiple cis-acting elements encoding positive and negative cell-specific regulatory systems.  (日)    [継承]
○キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Blotting, Northern (日) (読) [継承]
3. (英) Blotting, Southern (日) (読) [継承]
4. (英) Blotting, Western (日) (読) [継承]
5. (英) Bone and Bones (日) (読) [継承]
6. (英) Cartilage (日) (読) [継承]
7. (英) Crosses, Genetic (日) (読) [継承]
8. (英) Female (日) (読) [継承]
9. (英) Gene Dosage (日) (読) [継承]
10. (英) Gene Expression (日) (読) [継承]
11. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
12. (英) Green Fluorescent Proteins (日) (読) [継承]
13. (英) Immunohistochemistry (日) (読) [継承]
14. (英) In Situ Hybridization (日) (読) [継承]
15. (英) Kidney (日) (読) [継承]
16. (英) Leg Bones (日) (読) [継承]
17. (英) Luminescent Proteins (日) (読) [継承]
18. (英) Mammary Glands, Animal (日) (読) [継承]
19. (英) Mice (日) (読) [継承]
20. (英) Mice, Inbred C57BL (日) (読) [継承]
21. (英) Mice, Inbred DBA (日) (読) [継承]
22. (英) Mice, Transgenic (日) (読) [継承]
23. (英) Models, Genetic (日) (読) [継承]
24. (英) Osteopontin (日) (読) [継承]
25. (英) Placenta (日) (読) [継承]
26. (英) Pregnancy (日) (読) [継承]
27. (英) Promoter Regions, Genetic (日) (読) [継承]
28. (英) Recombinant Fusion Proteins (日) (読) [継承]
29. (英) Regulatory Sequences, Nucleic Acid (日) (読) [継承]
30. (英) Sialoglycoproteins (日) (読) [継承]
31. (英) Skin (日) (読) [継承]
32. (英) Spine (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □Journal of Bone and Mineral Research ([米国骨代謝学会])
(pISSN: 0884-0431, eISSN: 1523-4681)

○ISSN (任意): □0884-0431
ISSN: 0884-0431 (pISSN: 0884-0431, eISSN: 1523-4681)
Title: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Title(ISO): J Bone Miner Res
Supplier: American Society for Bone and Mineral Research
Publisher: Wiley
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○巻 (必須): □19 [継承]
○号 (必須): □1 [継承]
○頁 (必須): □78 88 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2004年 1月 初日 (平成 16年 1月 初日) [継承]
○URL (任意):
○DOI (任意): □10.1359/jbmr.2004.19.1.78    (→Scopusで検索) [継承]
○PMID (任意): □14753740    (→Scopusで検索) [継承]
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○備考 (任意): 1.(英) Article.Affiliation: Department of Pathology, Graduate School of Osaka University Medical School, Suita, Osaka, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yuji Higashibata, Takahiko Sakuma, Hirohisa Kawahata, Shinji Fujihara, Keiji Moriyama, Atsushi Okada, Takahiro Yasui, Kenjiro Kohri, Yukihiko Kitamura and Shintaro Nomura : Identification of Promoter Regions Involved in Cell- and Developmental Stage-Specific Osteopontin Expression in Bone, Kidney, Placenta, and Mammary Gland: An Analysis of Transgenic Mice, --- An analysis of transgenic mice. ---, Journal of Bone and Mineral Research, 19, 1, 78-88, 2004.
欧文冊子 ● Yuji Higashibata, Takahiko Sakuma, Hirohisa Kawahata, Shinji Fujihara, Keiji Moriyama, Atsushi Okada, Takahiro Yasui, Kenjiro Kohri, Yukihiko Kitamura and Shintaro Nomura : Identification of Promoter Regions Involved in Cell- and Developmental Stage-Specific Osteopontin Expression in Bone, Kidney, Placenta, and Mammary Gland: An Analysis of Transgenic Mice, --- An analysis of transgenic mice. ---, Journal of Bone and Mineral Research, 19, 1, 78-88, 2004.

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