『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.神経情報医学部門.情報統合医学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.鈴木 登志子 (->個人[山本 登志子])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Fujii Yutaka (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Miyano Masashi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Chen Lan-Ying (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Takahashi Tomohiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Watanabe Kikuko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Prostaglandin (PG) F synthase catalyzes the reduction of PGD2 to 9alpha,11beta-PGF2 and that of PGH2 to PGF2alpha on the same molecule. PGF synthase has at least two isoforms, the lung-type enzyme (Km value of 120 microM for PGD2 (Watanabe, K., Yoshida, R., Shimizu, T., and Hayaishi, O. (1985) J. Biol. Chem. 260, 7035-7041) and the liver-type one (Km value of 10 microM for PGD2 (Chen, L. -Y., Watanabe, K., and Hayaishi, O. (1992) Arch. Biochem. Biophys. 296, 17-26)). The liver-type enzyme was presently found to consist of a 969-base pair open reading frame coding for a 323-amino acid polypeptide with a Mr of 36,742. Sequence analysis indicated that the bovine liver PGF synthase had 87, 79, 77, and 76% identity with the bovine lung PGF synthase and human liver dihydrodiol dehydrogenase (DD) isozymes DD1, DD2, and DD4, respectively. Moreover, the amino acid sequence of the liver-type PGF synthase was identical with that of bovine liver DD3. The liver-type PGF synthase was expressed in COS-7 cells, and its recombinant enzyme had almost the same properties as the native enzyme. Furthermore, to investigate the nature of catalysis and/or substrate binding of PGF synthase, we constructed and characterized various mutant enzymes as follows: R27E, R91Q, H170C, R223L, K225S, S301R, and N306Y. Although the reductase activities toward PGH2 and phenanthrenequinone (PQ) of almost all mutants were not inactivated, the Km values of R27E, R91Q, H170C, R223L, and N306Y for PGD2 were increased from 15 to 110, 145, 75, 180, and 100 microM, respectively, indicating that Arg27, Arg91, His170, Arg223, and Asn306 are essential to give a low Km value for PGD2 of the liver-type PGF synthase and that these amino acid residues serve in the binding of PGD2. Moreover, the R223L mutant among these seven mutants especially has a profound effect on kcat for PGD2 reduction. The Km values of R223L, K225S, and S301R for PQ were about 2-10-fold lower than the wild-type value, indicating that the amino acid residues at 223, 225 and 301 serve in the binding of PQ to the enzyme. On the other hand, the Km value of H170C for PGH2 was 8-fold lower than that of the wild type, indicating that the amino acid residue at 170 is related to the binding of PGH2 to the enzyme and that Cys170 confer high affinity for PGH2. Additionally, the 5-fold increase in kcat/Km value of the N306Y mutant for PGH2 compared with the wild-type value suggests that the amino acid at 306 plays an important role in catalytic efficiency for PGH2.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Base Sequence (日) (読) [継承]
4. (英) Blotting, Northern (日) (読) [継承]
5. (英) COS Cells (日) (読) [継承]
6. (英) Cattle (日) (読) [継承]
7. (英) Chromatography, Gel (日) (読) [継承]
8. (英) Cloning, Molecular (日) (読) [継承]
9. (英) DNA, Complementary (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) Hydroxyprostaglandin Dehydrogenases (日) (読) [継承]
12. (英) Liver (日) (読) [継承]
13. (英) Molecular Sequence Data (日) (読) [継承]
14. (英) Mutagenesis, Site-Directed (日) (読) [継承]
15. (英) RNA, Messenger (日) (読) [継承]
16. (英) Recombinant Proteins (日) (読) [継承]
17. (英) Sequence Homology, Amino Acid (日) (読) [継承]
18. (英) Substrate Specificity (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 274 [継承]
(必須): 1 [継承]
(必須): 241 248 [継承]
都市 (任意):
年月日 (必須): 西暦 1999年 1月 1日 (平成 11年 1月 1日) [継承]
URL (任意):
DOI (任意): 10.1074/jbc.274.1.241    (→Scopusで検索) [継承]
PMID (任意): 9867836    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000077900200036 [継承]
Scopus (任意): 2-s2.0-0032957688 [継承]
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指導教員 (推奨):
備考 (任意): 1.(英) Article.DataBankList.DataBank.DataBankName: GENBANK  (日)    [継承]
2.(英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: D88749  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Toshiko Suzuki, Yutaka Fujii, Masashi Miyano, Lan-Ying Chen, Tomohiro Takahashi and Kikuko Watanabe : cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase., The Journal of Biological Chemistry, Vol.274, No.1, 241-248, 1999.
欧文冊子 ● Toshiko Suzuki, Yutaka Fujii, Masashi Miyano, Lan-Ying Chen, Tomohiro Takahashi and Kikuko Watanabe : cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase., The Journal of Biological Chemistry, Vol.274, No.1, 241-248, 1999.

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