| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.ゲノム機能研究センター (〜2008年3月31日/->組織[徳島大学.疾患プロテオゲノム研究センター])
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| ○著者 (必須): | 1. | 片岡 正俊
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| 2. | (英) Inoue Sonoko (日) (読)
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| 3. | (英) Kajimoto Kazuaki (日) (読)
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| 4. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 5. | 馬場 嘉信
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| ○題名 (必須): | □ | (英) Usefulness of microchip electrophoresis for reliable analyses of non-satndard DNA samples and subsequent on-chip enzymatic digestion (日)
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| ○要約 (任意): | □ | (英) The Hitachi SV1100 utilizes capillary electrophoresis on a microchip that is capable of rapidly sizing DNA fragments. Reproducibility of electrophoresis in different channels was shown by comparing the migration times of the internal controls, DNA fragments of 100 and 800 bp. The range of DNA sizing for this microchip is between 100 and 800 bp, and accuracy in sizing of a 322 bp DNA fragment of a pUC118 PvuII digest was observed, independent of DNA concentration. Although relatively good quantification of this fragment was observed with a DNA concentration of 1.83 ng.microL(-1), error increased in a dose-dependent manner. Furthermore, the feasibility of sequential analysis with this microchip was shown by the reproducibility of successive electrophoreses of the internal control in one channel. When the pUC118 PvuII digest was treated with endonuclease KpnI on the microchip for 10 min, sequential analysis showed that the 322 bp fragment completely disappeared and two peaks corresponding to the 130 and 192 bp fragments appeared. This analysis was performed within 4 min, and the peaks were estimated as 127 and 183 bp, respectively. These results indicate the potential of on-microchip endonuclease treatment of plasmid DNA with sequential analysis, offering high resolution in a short time. (日)
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| ○キーワード (推奨): | 1. | (英) DNA (日) (読)
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| 2. | (英) Deoxyribonucleases, Type II Site-Specific (日) (読)
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| 3. | (英) Electrophoresis, Capillary (日) (読)
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| 4. | (英) Plasmids (日) (読)
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| 5. | (英) Reproducibility of Results (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | European Journal of Biochemistry ([Federation of European Biochemical Societies])
(pISSN: 0014-2956, eISSN: 1432-1033)
| ○ISSN (任意): | □ | 0014-2956
ISSN: 0014-2956
(pISSN: 0014-2956, eISSN: 1432-1033) Title: European journal of biochemistryTitle(ISO): Eur J BiochemPublisher: Wiley-Blackwell Publishing Ltd (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 271
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| ○号 (必須): | □ | 11
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| ○頁 (必須): | □ | 2241 2247
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2004年 5月 10日 (平成 16年 5月 10日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1111/j.1432-1033.2004.04161.x (→Scopusで検索)
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| ○PMID (任意): | □ | 15153114 (→Scopusで検索)
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| ○WOS : | □ | 000221533600023
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Division of Gene Expression, Institute for Genome Research, The University of Tokushima, Kuramotocho 3-18, Tokushima 770-8503, Japan. kataoka@genome.tokushima-u.ac.jp (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Evaluation Studies (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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