『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=96840EID:96840, Map:0, LastModified:2012年10月24日(水) 20:11:19, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[稲垣 明浩], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.病態予防医学講座.生理機能学 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.医学部.医学科.病態予防医学講座.細胞生物学分野 [継承]
著者 (必須): 1.稲垣 明浩 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.生理学])
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2. (英) Yamaguchi, Souichirou (日) 山口 聡一郎 (読) やまぐち そういちろう
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3. (英) Ishikawa, Toru (日) 石川 透 (読) いしかわ とおる
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題名 (必須): (英) Amiloride-sensitive epithelial Na+ channel currents in surface cells of rat rectal colon.  (日)    [継承]
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要約 (任意): (英) Surface cells of the mammalian distal colon are shown to molecularly express the amiloride-sensitive epithelial Na+ channel composed of three homologous subunits (alpha-, beta-, and gamma-ENaC). However, because basic electrophysiological properties of amiloride-sensitive Na+ channels expressed in these cells are largely unknown at the cellular level, functional evidence for the involvement of the subunits in the native channels is incomplete. Using electrophysiological techniques, we have now characterized functional properties of native ENaC in surface cells of rectal colon (RC) of rats fed a normal Na+ diet. Ussing chamber experiments showed that apical amiloride inhibited a basal short-circuit current in mucosal preparation of RC with an apparent half-inhibition constant (Ki) value of 0.20 microM. RT-PCR analysis confirmed the presence of transcripts of alpha-, beta-, and gamma-rENaC in rectal mucosa. Whole cell patch-clamp experiments in surface cells of intact crypts acutely isolated from rectal mucosa identified an inward cationic current, which was inhibited by amiloride with a Ki value of 0.12 microM at a membrane potential of -64 mV, the inhibition being weakly voltage dependent. Conductance ratios of the currents were Li+ (1.8) > Na+ (1) > K+ ( approximately 0), respectively. Amiloride-sensitive current amplitude was almost the same at 15 or 150 mM extracellular Na+, suggesting a high Na+ affinity for current activation. These results are consistent with the hypothesis that a heterooligomer composed of alpha-, beta-, and gamma-ENaC may be the molecular basis of the native channels, which are responsible for amiloride-sensitive electrogenic Na+ absorption in rat rectal colon.  (日)    [継承]
キーワード (推奨): 1. (英) distal colon (日) (読) [継承]
2. (英) electrogenic Na+ absorption (日) (読) [継承]
3. (英) short-circuit current (日) (読) [継承]
4. (英) whole cell patch clamp (日) (読) [継承]
5. (英) alpha-beta-gamma-ENaC (日) (読) [継承]
発行所 (推奨): アメリカ生理学会 [継承]
誌名 (必須): American Journal of Physiology, Cell Physiology ([アメリカ生理学会])
(pISSN: 0363-6143, eISSN: 1522-1563)

ISSN (任意): 0363-6143
ISSN: 0363-6143 (pISSN: 0363-6143, eISSN: 1522-1563)
Title: American journal of physiology. Cell physiology
Title(ISO): Am J Physiol Cell Physiol
Publisher: American Physiological Society
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年月日 (必須): 西暦 2004年 0月 初日 (平成 16年 0月 初日) [継承]
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DOI (任意): 10.1152/ajpcell.00373.2003    (→Scopusで検索) [継承]
PMID (任意): 14576089    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akihiro Inagaki, Souichirou Yamaguchi and Toru Ishikawa : Amiloride-sensitive epithelial Na+ channel currents in surface cells of rat rectal colon., American Journal of Physiology, Cell Physiology, Vol.286, No.2, 380-390, 2004.
欧文冊子 ● Akihiro Inagaki, Souichirou Yamaguchi and Toru Ishikawa : Amiloride-sensitive epithelial Na+ channel currents in surface cells of rat rectal colon., American Journal of Physiology, Cell Physiology, Vol.286, No.2, 380-390, 2004.

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