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EID=96153EID:96153, Map:0, LastModified:2013年9月11日(水) 21:08:28, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[細井 和雄], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kurihara, Kinji (日) (読)
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2.細井 和雄
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3. (英) Kodama, Akiko (日) (読)
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4. (英) Ueha, Takao (日) (読)
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題名 (必須): (英) A new electrophoretic variant of a subunit of Na+,K+-ATPase from the submandibular gland of rats  (日)    [継承]
副題 (任意):
要約 (任意): (英) The alpha catalytic subunits of Na+/K(+)-ATPase were isolated from the kidney and brain of rats (alpha 1 and alpha 2, respectively). The antisera raised against these subunits were used as probes to analyze the isoform of catalytic subunits of Na+/K(+)-ATPase in various tissues of rats. Of 27 rat tissues examined, most had a catalytic subunit identical to alpha 1 but some, such as the nervous and muscle tissues, had both alpha 1 and alpha 2 isoforms as judged by their reactivities to antisera and their electrophoretic mobility. We found that the submandibular gland contained a new electrophoretic variant of immunoreactive alpha subunit (designated alpha(S) in this report) in addition to alpha 1 identical to those found in kidney and brain. The new variant, alpha(S), strongly cross-reacted with anti-alpha 1 antiserum, but to a lesser extent with anti-alpha 2 antiserum. The alpha(S) had a molecular mass which was found to be slightly less (approx. 90 kDa) than brain and kidney alpha 1. We examined whether or not the alpha(S) is formed by proteolytic cleavage of alpha subunits during preparation and concluded that this is not the case. The alpha(S) reacted with [gamma-32P]ATP, resulting in the formation of radioactive alpha subunit which was stabilized by 2 mM ouabain but which was labile in the presence of 70 mM potassium chloride. Since N-terminal amino acid sequence of alpha(S) protein [G()DKY()PAAVS] corresponds exactly and uniquely with the sequence of the alpha 1 chain between residues 1 and 11, it is very probable that alpha(S) protein originated from alpha 1 protein following the post-translational processing.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Brain (日) (読) [継承]
5. (英) Genetic Variation (日) (読) [継承]
6. (英) Immune Sera (日) (読) [継承]
7. (英) Isoenzymes (日) (読) [継承]
8. (英) Kidney (日) (読) [継承]
9. (英) Macromolecular Substances (日) (読) [継承]
10. (英) Male (日) (読) [継承]
11. (英) Microsomes (日) (読) [継承]
12. (英) Molecular Sequence Data (日) (読) [継承]
13. (英) Organ Specificity (日) (読) [継承]
14. (英) Rats (日) (読) [継承]
15. (英) Rats, Inbred Strains (日) (読) [継承]
16. (英) Sequence Homology, Nucleic Acid (日) (読) [継承]
17. (英) Sodium-Potassium-Exchanging ATPase (日) (読) [継承]
18. (英) Submandibular Gland (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology ([Elsevier Science])
(pISSN: 0167-4838)

ISSN (任意): 0167-4838
ISSN: 0167-4838 (pISSN: 0167-4838)
Title: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Publisher: Elsevier BV
 (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 1039 [継承]
(必須): 2 [継承]
(必須): 234 240 [継承]
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年月日 (必須): 西暦 1990年 6月 19日 (平成 2年 6月 19日) [継承]
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DOI (任意): 10.1016/0167-4838(90)90191-H    (→Scopusで検索) [継承]
PMID (任意): 2163680    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Oral Physiology, Meikai University, School of Dentistry, Saitama, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kinji Kurihara, Kazuo Hosoi, Akiko Kodama and Takao Ueha : A new electrophoretic variant of a subunit of Na+,K+-ATPase from the submandibular gland of rats, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol.1039, No.2, 234-240, 1990.
欧文冊子 ● Kinji Kurihara, Kazuo Hosoi, Akiko Kodama and Takao Ueha : A new electrophoretic variant of a subunit of Na+,K+-ATPase from the submandibular gland of rats, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol.1039, No.2, 234-240, 1990.

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