『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=93914EID:93914, Map:0, LastModified:2018年5月23日(水) 17:08:55, Operator:[[ADMIN]], Avail:TRUE, Censor:0, Owner:[[副研究部長]/[大学院ヘルスバイオサイエンス研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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組織 (推奨): 1.徳島大学.医療技術短期大学部 [継承]
2.徳島大学.医学部 [継承]
著者 (必須): 1.吉永 純子
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2. (英) Yasuoka Susumu (日) 安岡 劭 (読) やすおか すすむ
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3.中堀 豊
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題名 (必須): (英) Fibrinogenolytic activity of a novel trypsin-like enzyme found in human airway  (日)    [継承]
副題 (任意):
要約 (任意): (英) Previously we isolated a new trypsin-like enzyme designated human airway trypsin-like protease (HAT) from human sputum. In this study, we examined in vitro whether HAT was related to the prevention of fibrin deposition in the airway lumen by cleaving fibrinogen. In mucoid sputum samples from patients with chronic airway diseases, the concentration of fibrinogen, as measured by ELISA, was in the range of 2-20 micrograms/ml, and trypsin-like activity, as measured by spectrofluorometry was in the range of 10-50 milliunits (mU)/ml. We showed by gel filtration that the trypsin-like activity of mucoid sputum was mainly due to HAT. We examined the effects of HAT on human fibrinogen at pH 7.4 and 8.6. Fibrinogen was used at concentrations of 4-2,000 micrograms/ml and HAT purified from sputum at concentrations of 0.6-10 mU/ml. As shown by SDS-polyacrylamide gel electrophoresis, HAT cleaved fibrinogen, especially its alpha-chain, regardless of the concentration of fibrinogen. Pretreatment of fibrinogen with HAT resulted in a decrease or complete loss of its thrombin-induced clotting capacity, depending on the duration of pretreatment with HAT and the concentration of HAT. From these results we postulated that HAT may participate in the anticoagulation process within the airway, especially at the level of the mucous membrane, by cleaving fibrinogen transported from the blood stream.  (日)    [継承]
キーワード (推奨): 1. (英) Albumins (日) (読) [継承]
2. (英) Asthma (日) (読) [継承]
3. (英) Chromatography, Gel (日) (読) [継承]
4. (英) Fibrinogen (日) (読) [継承]
5. (英) Fibrinopeptide A (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7. (英) Lung Diseases, Obstructive (日) (読) [継承]
8. (英) Pancreatic Elastase (日) (読) [継承]
9. (英) Serine Endopeptidases (日) (読) [継承]
10. (英) Sputum (日) (読) [継承]
11. (英) Thrombin (日) (読) [継承]
発行所 (推奨): 徳島大学 [継承]
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1343-1420
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J Med Invest
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 45 [継承]
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(必須): 77 86 [継承]
都市 (任意): 徳島 (Tokushima/[日本国]) [継承]
年月日 (必須): 西暦 1998年 8月 初日 (平成 10年 8月 初日) [継承]
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PMID (任意): 9864967    (→Scopusで検索) [継承]
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機関リポジトリ : 111619 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Sumiko Yoshinaga, Susumu Yasuoka and Yutaka Nakahori : Fibrinogenolytic activity of a novel trypsin-like enzyme found in human airway, The Journal of Medical Investigation : JMI, Vol.45, No.1-4, 77-86, 1998.
欧文冊子 ● Sumiko Yoshinaga, Susumu Yasuoka and Yutaka Nakahori : Fibrinogenolytic activity of a novel trypsin-like enzyme found in human airway, The Journal of Medical Investigation : JMI, Vol.45, No.1-4, 77-86, 1998.

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