『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Tsuruo Yoshihiro (日) (読)
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2.石村 和敬
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3.森田 恭二
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題名 (必須): (英) Influence of serum-free culture conditions on subcellular localization of steroid 5alpha-reductase in rat C6 glioma cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Rat C6 glioma cells are considered to be well characterized, and therefore commonly used as a model system to investigate the function of glial cells. However, recent study has shown that an alteration in the expression of their phenotypic antigens is observed when the cells are maintained under the serum-free conditions, proposing the possibility that various properties of glioma cells can be altered by the growth conditions. To test this possibility, the effects of serum-free culture conditions on the expression of steroid 5alpha-reductase (5alpha-R) type 1 isozyme in glioma cells were examined using immunocytochemical technique. Immunoreactivity of 5alpha-R type 1 was confined to the perinuclear region of glioma cells cultured in serum-containing medium, and observed in the cytoplasmic space as well as the perinuclear region of the cells cultured in serum-free medium. In contrast, serum deprivation failed to affect the expression of phenotypic antigens, glial fibrillary acidic protein (GFAP) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase). Further studies showed that the expression of cytoplasmic 5alpha-R immunoreactivity induced by serum deprivation was reversible, and might be attributed to removal of serum proteins rather than biologically active small molecules from culture medium. This alteration in the expression of 5alpha-R immunoreactivity is therefore considered to reflect the translocation of the enzyme from the perinuclear region to the cell cytoplasm rather than the induction of cytoplasmic enzyme, and suggest that the culture conditions cause an alteration in the subcellular localization of 5alpha-R type 1 isozyme without phenotypic change of the glioma cell.  (日)    [継承]
キーワード (推奨): 1. (英) 2',3'-Cyclic-Nucleotide Phosphodiesterases (日) (読) [継承]
2. (英) 3-Oxo-5-alpha-Steroid 4-Dehydrogenase (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Antibodies (日) (読) [継承]
5. (英) Biological Markers (日) (読) [継承]
6. (英) Cell Nucleus (日) (読) [継承]
7. (英) Cell Size (日) (読) [継承]
8. (英) Culture Media, Serum-Free (日) (読) [継承]
9. (英) Cytoplasm (日) (読) [継承]
10. (英) Glial Fibrillary Acidic Protein (日) (読) [継承]
11. (英) Glioma (日) (読) [継承]
12.免疫組織化学 (immunohistochemistry) [継承]
13. (英) Intracellular Fluid (日) (読) [継承]
14. (英) Isoenzymes (日) (読) [継承]
15. (英) Neuroglia (日) (読) [継承]
16. (英) Rats (日) (読) [継承]
17. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Brain Research ([Elsevier Science])
(pISSN: 0006-8993, eISSN: 1872-6240)

ISSN (任意): 0006-8993
ISSN: 0006-8993 (pISSN: 0006-8993, eISSN: 1872-6240)
Title: Brain research
Title(ISO): Brain Res
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 801 [継承]
(必須): 1-2 [継承]
(必須): 130 136 [継承]
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年月日 (必須): 西暦 1998年 8月 10日 (平成 10年 8月 10日) [継承]
URL (任意):
DOI (任意): 10.1016/S0006-8993(98)00555-1    (→Scopusで検索) [継承]
PMID (任意): 9729333    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000075451100016 [継承]
Scopus (任意): 2-s2.0-0032504603 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yoshihiro Tsuruo, Kazunori Ishimura and Kyoji Morita : Influence of serum-free culture conditions on subcellular localization of steroid 5alpha-reductase in rat C6 glioma cells, Brain Research, Vol.801, No.1-2, 130-136, 1998.
欧文冊子 ● Yoshihiro Tsuruo, Kazunori Ishimura and Kyoji Morita : Influence of serum-free culture conditions on subcellular localization of steroid 5alpha-reductase in rat C6 glioma cells, Brain Research, Vol.801, No.1-2, 130-136, 1998.

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