『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=92083EID:92083, Map:0, LastModified:2013年2月5日(火) 10:37:37, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[土屋 浩一郎], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.医学部.医学科.病態情報医学講座 [継承]
著者 (必須): 1.土屋 浩一郎 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品機能生化学]/[徳島大学.医学部.医学科.病態情報医学講座])
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2. (英) Kirima Kazuyoshi (日) 桐間一嘉 (読) きりまかずよし
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3.吉栖 正典
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4.玉置 俊晃
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5. (英) Mason Ronald (日) (読)
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題名 (必須): (英) The role of thiol and nitrosothiol compounds in the NO-forming reactions of the iron-MGD complex.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The object of the present study is to investigate whether the physiologically dominant thiol compounds such as GSH and cysteine or their nitrosothiol compounds affect the formation of the iron- N -methyl-D-glucamine dithiocarbamate [(MGD)(2)Fe(2+)]-nitric oxide complex. The present study provided experimental evidence that physiological concentrations of GSH (approx. 5 mM) and L-cysteine (approx. 0.5 mM) accelerated the formation of the (MGD)(2)Fe(2+)-NO complex from nitrite by two and three times respectively. The rate constants for the reduction of (MGD)(3)Fe(3+) to (MGD)(2)Fe(2+) by GSH and cysteine were calculated as 1.3 and 2.0x10(2) M(-1).s(-1) respectively. Furthermore, depletion of GSH was demonstrated in PC12 cells, and thiol compounds enhanced the formation of reactive oxygen species by the (MGD)(2)Fe(2+) complex by accelerating its redox turnover. The main effect of the physiological concentration of thiols was the reduction of (MGD)(3)Fe(3+). S -nitrosoglutathione spontaneously reacted with (MGD)(2)Fe(2+) to produce the (MGD)(2)Fe(2+)-NO complex with a 1:2 stoichiometry. In fact, (MGD)(2)Fe(2+) was as good an indicator of nitrosothiols as it was of NO itself. The present study elucidates the difficulties of utilizing the (MGD)(2)Fe(2+) complex for the quantification of NO in biological samples, especially in vivo.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Electron Spin Resonance Spectroscopy (日) (読) [継承]
3. (英) Glutathione (日) (読) [継承]
4. (英) Nitric Oxide (日) (読) [継承]
5. (英) Nitroso Compounds (日) (読) [継承]
6. (英) Oxygen (日) (読) [継承]
7. (英) PC12 Cells (日) (読) [継承]
8. (英) Rats (日) (読) [継承]
9. (英) Sorbitol (日) (読) [継承]
10. (英) Spin Labels (日) (読) [継承]
11. (英) Sulfhydryl Compounds (日) (読) [継承]
12. (英) Thiocarbamates (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 367 [継承]
(必須): Pt 3 [継承]
(必須): 771 779 [継承]
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年月日 (必須): 西暦 2002年 11月 1日 (平成 14年 11月 1日) [継承]
URL (任意):
DOI (任意): 10.1042/BJ20020310    (→Scopusで検索) [継承]
PMID (任意): 12141947    (→Scopusで検索) [継承]
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WOS (任意): 000179177900022 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Pharmacology, University of Tokushima School of Medicine, Tokushima 770-8503, Japan. tsuchiya@basic.med.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) OtherID: PMC1222930  (日)    [継承]

標準的な表示

和文冊子 ● Koichiro Tsuchiya, Kazuyoshi Kirima, Masanori Yoshizumi, Toshiaki Tamaki and Ronald Mason : The role of thiol and nitrosothiol compounds in the NO-forming reactions of the iron-MGD complex., The Biochemical Journal, Vol.367, No.Pt 3, 771-779, 2002.
欧文冊子 ● Koichiro Tsuchiya, Kazuyoshi Kirima, Masanori Yoshizumi, Toshiaki Tamaki and Ronald Mason : The role of thiol and nitrosothiol compounds in the NO-forming reactions of the iron-MGD complex., The Biochemical Journal, Vol.367, No.Pt 3, 771-779, 2002.

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