『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体情報薬科学部門.分子情報薬学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.柴田 瑩
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2. (英) Murata Syuichi (日) (読)
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3.植野 哲 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.総合薬学教育学系.総合薬学研究推進学])
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4. (英) Liu Shaoqiqn (日) (読)
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5. (英) Futaki Shiroh (日) (読)
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6.馬場 嘉信
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題名 (必須): (英) Synthetic copoly(Lys/Phe) and poly(Lys) translocate through lipid bilayer membranes  (日)    [継承]
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要約 (任意): (英) Several membrane-transporting peptides (MTP) containing basic amino acid residues such as Lys and Arg that carry macromolecules such as DNA and proteins across cell plasma membranes by an unknown mechanism have been actively studied. On the basis of these results, we have been investigating the translocation ability of synthetic polypeptides, copoly(Lys/Phe) and poly(Lys), through negatively charged phospholipid (soybean phospholipid (SBPL)) bilayer membranes by zeta potential analysis, circular dichroism (CD) spectroscopy, fluorescence spectroscopy, an electrophysiology technique, and confocal laser scanning microscopy (CLSM). The binding of these polypeptides to the membrane, which is the first step for translocation across the membrane, resulted in the conformational transition of the polypeptide from a random coil form or helix-poor form to a helix-rich form. The fluorescence studies demonstrated that the time-dependent decrease in the fluorescence intensities of the FITC-labeled polypeptides bound to the SBPL liposome reflected translocation of the polypeptide across the lipid bilayer with the low dielectric constant. Both the rate constant and the efficiency of the polypeptide translocation across the lipid bilayer were greater for copoly(Lys/Phe) than for poly(Lys). These results suggest that the random incorporation of the hydrophobic Phe residue into the positively charged Lys chain results in a lowering of the potential barrier for passage of the polypeptide in the hydrophobic core portion of the lipid bilayer. We presented the first direct observation that the positively charged polypeptides, copoly(Lys/Phe) (MW: 41,500) and poly(Lys) (MW: 23,400), could translocate across the lipid bilayer membrane.  (日)    [継承]
キーワード (推奨): 1. (英) Positively charged polypeptide (日) (読) [継承]
2. (英) Lipid membrane (日) (読) [継承]
3. (英) Translocation (日) (読) [継承]
4. (英) Secondary structure (日) (読) [継承]
5. (英) Micropolarity (日) (読) [継承]
発行所 (推奨): Elsevier Science B.V. [継承]
誌名 (必須): Biochimica et Biophysica Acta (BBA) - Biomembranes ([Elsevier Science])
(pISSN: 0005-2736, eISSN: 1879-2642)

ISSN (任意): 0005-2736
ISSN: 0005-2736 (pISSN: 0005-2736, eISSN: 1879-2642)
Title: Biochimica et biophysica acta. Biomembranes
Title(ISO): Biochim Biophys Acta Biomembr
Publisher: Elsevier BV
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(必須): 147 155 [継承]
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年月日 (必須): 西暦 2003年 9月 初日 (平成 15年 9月 初日) [継承]
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DOI (任意): 10.1016/j.bbamem.2003.08.003    (→Scopusで検索) [継承]
PMID (任意): 14561472    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Faculty of Pharmaceutical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan. ashibata@ph.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Akira Shibata, Syuichi Murata, Satoru Ueno, Shaoqiqn Liu, Shiroh Futaki and Yoshinobu Baba : Synthetic copoly(Lys/Phe) and poly(Lys) translocate through lipid bilayer membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1616, No.2, 147-155, 2003.
欧文冊子 ● Akira Shibata, Syuichi Murata, Satoru Ueno, Shaoqiqn Liu, Shiroh Futaki and Yoshinobu Baba : Synthetic copoly(Lys/Phe) and poly(Lys) translocate through lipid bilayer membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1616, No.2, 147-155, 2003.

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