『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=90363)

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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Tai T.C. (日) (読)
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2.森田 恭二
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3. (英) Wong Dona L (日) (読)
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題名 (必須): (英) Role of Egr-1 in cAMP-dependent protein kinase regulation of the phenylethanolamine N-methyltransferase gene  (日)    [継承]
副題 (任意):
要約 (任意): (英) The molecular mechanism by which cAMP activates the rat phenylethanolamine N-methyltransferase (PNMT) gene was examined by transient transfection of the wild-type rat PNMT promoter-luciferase reporter gene construct pGL3RP893 into PC12 cells. Forskolin treatment (10 microM) of the transfected cells for 3--6 h maximally induced luciferase threefold. Induction by forskolin was mimicked by the cAMP analog, 8-Br-cAMP, and prevented in PC12 cells pretreated with the protein kinase A (PKA) inhibitor H-89 or co-transfected with an expression construct for PKI, a polypeptide inhibitor of PKA. Furthermore, forskolin did not activate the PNMT promoter when the 893 bp PNMT promoter-reporter gene construct was transfected into the PKA-deficient cell line, A126. Detailed examination of the forskolin responsiveness of PNMT constructs harboring > or = 60 bp and < 893 bp of PNMT promoter demonstrated that the cAMP-responsive element(s) lay between < 392 bp and > or =60 bp. Within this region of the promoter lies a functional binding element for Egr-1, a transcriptional activator of the PNMT gene. Forskolin treatment of PC12 cells also rapidly increased nuclear levels of Egr-1 and the catalytic subunit of PKA (PKA-C), with the rise in PKA-C preceding that of Egr-1. Mutation of the --165 bp Egr-1 site markedly decreased forskolin activation of the PNMT promoter. These findings demonstrate that the rat PNMT gene promoter can be activated via the cAMP-PKA signal transduction pathway, mediated by the immediate early gene transcription factor, Egr-1.  (日)    [継承]
キーワード (推奨): 1. (英) 8-Bromo Cyclic Adenosine Monophosphate (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Colforsin (日) (読) [継承]
4. (英) Cyclic AMP-Dependent Protein Kinases (日) (読) [継承]
5. (英) DNA-Binding Proteins (日) (読) [継承]
6. (英) Early Growth Response Protein 1 (日) (読) [継承]
7. (英) Gene Expression Regulation, Enzymologic (日) (読) [継承]
8. (英) Genes, Reporter (日) (読) [継承]
9. (英) Immediate-Early Proteins (日) (読) [継承]
10. (英) Isoquinolines (日) (読) [継承]
11. (英) Kinetics (日) (読) [継承]
12. (英) Luciferases (日) (読) [継承]
13. (英) Mitogen-Activated Protein Kinases (日) (読) [継承]
14. (英) PC12 Cells (日) (読) [継承]
15. (英) Phenylethanolamine N-Methyltransferase (日) (読) [継承]
16. (英) Promoter Regions, Genetic (日) (読) [継承]
17. (英) Rats (日) (読) [継承]
18. (英) Signal Transduction (日) (読) [継承]
19. (英) Sulfonamides (日) (読) [継承]
20. (英) Transcription Factors (日) (読) [継承]
21. (英) Transfection (日) (読) [継承]
22. (英) beta-Galactosidase (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Neurochemistry ([The International Society for Neurochemistry])
(pISSN: 0022-3042, eISSN: 1471-4159)

ISSN (任意): 0022-3042
ISSN: 0022-3042 (pISSN: 0022-3042, eISSN: 1471-4159)
Title: Journal of neurochemistry
Title(ISO): J. Neurochem.
Supplier: International Society for Neurochemistry
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 76 [継承]
(必須): 6 [継承]
(必須): 1851 1859 [継承]
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年月日 (必須): 西暦 2001年 3月 初日 (平成 13年 3月 初日) [継承]
URL (任意):
DOI (任意): 10.1046/j.1471-4159.2001.00189.x    (→Scopusで検索) [継承]
PMID (任意): 11259503    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]

標準的な表示

和文冊子 ● T.C. Tai, Kyoji Morita and L Dona Wong : Role of Egr-1 in cAMP-dependent protein kinase regulation of the phenylethanolamine N-methyltransferase gene, Journal of Neurochemistry, Vol.76, No.6, 1851-1859, 2001.
欧文冊子 ● T.C. Tai, Kyoji Morita and L Dona Wong : Role of Egr-1 in cAMP-dependent protein kinase regulation of the phenylethanolamine N-methyltransferase gene, Journal of Neurochemistry, Vol.76, No.6, 1851-1859, 2001.

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