『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=88274EID:88274, Map:0, LastModified:2014年4月15日(火) 14:03:35, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[工藤 景子], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔再建医学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.北岡 栄一郎
役割 (任意): 共著 [継承]
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2.里村 一人
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[継承]
3. (英) Hayashi Eiji (日) (読)
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[継承]
4.工藤 景子 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.口腔外科学])
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学籍番号 (推奨):
[継承]
5. (英) Tobiume Satoru (日) (読)
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[継承]
6.長山 勝
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Establishment and charcterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice  (日)    [継承]
副題 (任意):
要約 (任意): (英) Complete understanding of the physiology and pathology of the cartilage is essential to establish treatments for a variety of cartilage disorders and defects such as rheumatoid arthritis, congenital malformations, and tumors of cartilage. Although synthetic materials have been used in many cases, they possess inherent problems including wear of the materials and low mechanical strength. Autograft has been considered very effective to overcome these problems. However, the limitation of the transplant volume is a major problem in autograft to be overcome. The costal cartilage is the most serious candidate for donor site transplantation, since it is the largest permanent hyaline cartilage in the body. To investigate the possibility using the costal cartilage as a transplant source, we have established and characterized three mouse chondrocyte cell lines (MCC-2, MCC-5, and MCC-35) derived from the costal cartilage of 8-week-old male SV40 large T-antigen transgenic mice. At confluence, all the cell lines formed nodules that could be positively stained with alcian blue (pH 2.5). The size of nodules gradually increased during culturing time. After 2 and 6 weeks of culture, RT-PCR analysis demonstrated that all three cell lines expressed mRNA from the cartilage-specific genes for type II collagen, type XI collagen, aggrecan, and link protein. Furthermore, type X collagen expression was detected in MCC-5 and MCC-35 but not in MCC-2. Any phenotypic changes were not observed over 31 cell divisions. Immunocytochemistry showed further that MCC-2, MCC-5, and MCC-35 produced cartilage-specific proteins type II collagen and type XI collagen, while in addition MCC-5 and MCC-35 produced type X collagen. Treatment with 1alpha, 25-dihydroxyvitamin D(3) inhibited cell proliferation and differentiation of the three cell lines in a dose-dependent manner. These phenotypic characteristics have been found consistent with chondrocyte cell lines established from cartilage tissues other than costal cartilage. In conclusion, costal cartilage shows phenotypic similarities to other cartilages, i.e., articular cartilage and embryonic limbs, suggesting that costal cartilage may be very useful as the donor transplantation site for the treatment of cartilage disorders. Furthermore, the cell lines established in this study are also beneficial in basic research of cartilage physiology and pathology.  (日)    [継承]
キーワード (推奨): 1. (英) Alkaline Phosphatase (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antigens, Polyomavirus Transforming (日) (読) [継承]
4. (英) Biological Markers (日) (読) [継承]
5. (英) Calcitriol (日) (読) [継承]
6. (英) Cartilage (日) (読) [継承]
7. (英) Cell Division (日) (読) [継承]
8. (英) Cell Line (日) (読) [継承]
9. (英) Chondrocytes (日) (読) [継承]
10. (英) Collagen (日) (読) [継承]
11. (英) Extracellular Matrix (日) (読) [継承]
12. (英) Gene Expression (日) (読) [継承]
13. (英) Male (日) (読) [継承]
14. (英) Mice (日) (読) [継承]
15. (英) Mice, Transgenic (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Cellular Biochemistry ([Wiley-Liss, Inc.])
(pISSN: 0730-2312, eISSN: 1097-4644)

ISSN (任意): 0730-2312
ISSN: 0730-2312 (pISSN: 0730-2312, eISSN: 1097-4644)
Title: Journal of cellular biochemistry
Title(ISO): J Cell Biochem
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2001年 0月 初日 (平成 13年 0月 初日) [継承]
URL (任意):
DOI (任意): 10.1002/jcb.1075    (→Scopusで検索) [継承]
PMID (任意): 11329612    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000168462100001 [継承]
Scopus (任意): 2-s2.0-0035016352 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Eiichiro Kitaoka, Kazuhito Satomura, Eiji Hayashi, Keiko Kudoh, Satoru Tobiume and Masaru Nagayama : Establishment and charcterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice, Journal of Cellular Biochemistry, Vol.81, No.4, 571-582, 2001.
欧文冊子 ● Eiichiro Kitaoka, Kazuhito Satomura, Eiji Hayashi, Keiko Kudoh, Satoru Tobiume and Masaru Nagayama : Establishment and charcterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice, Journal of Cellular Biochemistry, Vol.81, No.4, 571-582, 2001.

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