『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.感覚運動系病態医学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.松井 好人
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Chansky A. Howard (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Barahmand-Pour Fariba (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Zielinska-Kwiatkowska Anna (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Tsumaki Noriyuki (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Myoui Akira (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Yoshikawa Hideki (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Yang Liu (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
9. (英) Eyre R. David (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG  (日)    [継承]
副題 (任意):
要約 (任意): (英) A specific t(21;22) chromosomal translocation creates the chimeric EWS/ERG gene in some cases of Ewing's sarcoma. In the resultant EWS/ERG fusion protein, the N-terminal part of the ETS family protein ERG is replaced by the N terminus of the RNA-binding protein EWS. We found that both the EWS/ERG and COL11A2 genes are expressed in the Ewing's sarcoma cell line, CADO-ES1. To investigate a potential role for EWS/ERG in COL11A2 gene expression, we characterized the COL11A2 promoter and tested the ability of wild-type ERG and EWS/ERG sarcoma fusion protein to transactivate COL11A2 promoter using a luciferase assay. We found that expression of EWS/ERG, but not wild-type ERG, transactivated the COL11A2 promoter and that this transactivation required not only the N-terminal region of EWS but also an intact DNA-binding domain from ERG. Electrophoretic mobility shift assay using COL11A2 promoter sequence showed involvement of EWS/ERG in the formation of DNA-protein complexes, and chromatin immunoprecipitation assay revealed direct interaction between COL11A2 promoter and EWS/ERG fusion protein in vivo. EWS/ERG, but not wild-type ERG, bound to RNA polymerase II. Treatment of cells with the histone deacetylase inhibitor trichostatin A enabled ERG to transactivate the COL11A2 promoter, therefore abolishing the differential effects of EWS/ERG and ERG. Taken together, these findings indicate that the COL11A2 gene is regulated both by potential ERG association with a histone deacetylase complex and by direct EWS/ERG recruitment of RNA polymerase II.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Cell Line (日) (読) [継承]
4. (英) Collagen (日) (読) [継承]
5. (英) DNA Primers (日) (読) [継承]
6. (英) DNA-Binding Proteins (日) (読) [継承]
7. (英) Electrophoretic Mobility Shift Assay (日) (読) [継承]
8. (英) Gene Expression Regulation (日) (読) [継承]
9. (英) Histone Deacetylases (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) Mice (日) (読) [継承]
12. (英) Oncogene Proteins (日) (読) [継承]
13. (英) Oncogene Proteins, Fusion (日) (読) [継承]
14. (英) Promoter Regions, Genetic (日) (読) [継承]
15. (英) RNA Polymerase II (日) (読) [継承]
16. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
17. (英) Trans-Activators (日) (読) [継承]
18. (英) Transcription Factors (日) (読) [継承]
19. (英) Transcription, Genetic (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 278 [継承]
(必須): 13 [継承]
(必須): 11369 11375 [継承]
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年月日 (必須): 西暦 2003年 3月 28日 (平成 15年 3月 28日) [継承]
URL (任意):
DOI (任意): 10.1074/jbc.M300164200    (→Scopusで検索) [継承]
PMID (任意): 12554743    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000181855400065 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Orthopedics and Sports Medicine, University of Washington School of Medicine, Seattle, Washington 98195-6500, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, Non-P.H.S.  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]

標準的な表示

和文冊子 ● Yoshito Matsui, Howard A. Chansky, Fariba Barahmand-Pour, Anna Zielinska-Kwiatkowska, Noriyuki Tsumaki, Akira Myoui, Hideki Yoshikawa, Liu Yang and David R. Eyre : COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG, The Journal of Biological Chemistry, Vol.278, No.13, 11369-11375, 2003.
欧文冊子 ● Yoshito Matsui, Howard A. Chansky, Fariba Barahmand-Pour, Anna Zielinska-Kwiatkowska, Noriyuki Tsumaki, Akira Myoui, Hideki Yoshikawa, Liu Yang and David R. Eyre : COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG, The Journal of Biological Chemistry, Vol.278, No.13, 11369-11375, 2003.

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