『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨): 1.徳島大学.工学部.生物工学科.生物反応工学講座 [継承]
著者 (必須): 1.坂東 美和
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2. (英) Matsuoka Atsushi (日) (読)
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3.辻 明彦
役割 (任意): (英)   (日) 計画,解析,考察,論文執筆指導   [継承]
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4.松田 佳子
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題名 (必須): (英) The proprotein convertase PACE4 is upregulated by PDGF-BB in megakaryocytes: gene expression of PACE4 and furin is regulated differently in Dami cells  (日)    [継承]
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要約 (任意): (英) The differentiation of megakaryocytes into platelets is highly regulated by many cytokines and growth factors. PACE4 and furin are Ca(2+)-dependent serine endoproteases belonging to the subtilisin-like proprotein convertase (SPC) family. These enzymes are involved in the proteolytic activation of proteins that play essential roles in cell growth and differentiation. In this study, we examined the expression of PACE4 and furin during the differentiation of megakaryoblastic cell lines, Dami and HEL cells, induced by phorbol 12-myristate 13-acetate (PMA). PMA stimulates not only the expression of platelet-derived growth factor-B (PDGF-B) mRNA, but also PACE4 mRNA in these cell lines. The expression of PACE4 transcripts (both the PACE4A and PACE4C/CS isoforms) was upregulated more than 4-fold by PMA. Moreover, direct treatment with PDGF-BB also resulted in an increase in the level of PACE4 mRNA. Further, the effect of PDGF-BB on PACE4 expression was confirmed by promoter assay of the PACE4 gene. Although the furin mRNA level was increased by TGF-beta1 in Dami cells, it was not affected by PDGF-BB. These results indicate for the first time that PACE4 expression is specifically upregulated by PDGF-BB in differentiated megakaryoblasts, suggesting a unique role for PACE4 in platelet production.  (日) 血小板の分化過程において,巨核芽球から血小板への分化は多くのサイトカインや分化因子によって高度に制御されている.本研究では,巨核芽球のモデル細胞であるDami, HEL細胞を用いて,分化因子前駆体を活性化するプロプロテインコンベルターゼ(SPC)の発現制御について解析した.SPCの中で,特にPACE4は細胞自身から分泌されるPDGFによって著明に発現量が増加することが判明し,PACE4は血小板の分化において重要な役割を持つことが示された   [継承]
キーワード (推奨): 1. (英) megakaryocytes (日) (読) [継承]
2. (英) PACE4 (日) (読) [継承]
3. (英) PDGF-BB (日) (読) [継承]
4. (英) proprotein convertase (日) (読) [継承]
5. (英) transcriprional regulation (日) (読) [継承]
発行所 (推奨): (英) Japanese Biochemical Society (日) (読) [継承]
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 0021-924X
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 132 [継承]
(必須): 1 [継承]
(必須): 127 134 [継承]
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年月日 (必須): 西暦 2002年 7月 1日 (平成 14年 7月 1日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/50000577945/ [継承]
DOI (任意):
PMID (任意): 12097169    (→Scopusで検索) [継承]
NAID (任意): 50000577945 [継承]
WOS (任意): 000176839000017 [継承]
Scopus (任意): 2-s2.0-0036330386 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Miwa Bando, Atsushi Matsuoka, Akihiko Tsuji and Yoshiko Matsuda : The proprotein convertase PACE4 is upregulated by PDGF-BB in megakaryocytes: gene expression of PACE4 and furin is regulated differently in Dami cells, The Journal of Biochemistry, Vol.132, No.1, 127-134, 2002.
欧文冊子 ● Miwa Bando, Atsushi Matsuoka, Akihiko Tsuji and Yoshiko Matsuda : The proprotein convertase PACE4 is upregulated by PDGF-BB in megakaryocytes: gene expression of PACE4 and furin is regulated differently in Dami cells, The Journal of Biochemistry, Vol.132, No.1, 127-134, 2002.

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