『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=84793EID:84793, Map:0, LastModified:2013年9月11日(水) 20:11:54, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[山﨑 尚志], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.薬学部.製薬化学科.薬品素材学講座 (〜2006年3月31日) [継承]
著者 (必須): 1.橋本 満 ([熊本大学])
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2.篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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3.真島 英司 (アプロサイエンス)
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4. (英) Hatanaka Takashi (日) (読)
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5.山﨑 尚志 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.薬物治療学])
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6.寺田 弘 ([東京理科大学])
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題名 (必須): (英) Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers.  (日)    [継承]
副題 (任意):
要約 (任意): (英) To characterize the transport mechanism mediated by the mammalian mitochondrial ADP/ATP carrier (AAC), we tried to express bovine heart mitochondrial AAC (bhAAC) in Saccharomyces cerevisiae. The open reading frame of the bhAAC was introduced into the haploid strain WB-12, in which intrinsic AAC genes were disrupted. Growth of the transformant was very low in glycerol medium, and a little amount of bhAAC was detected in the mitochondrial membrane. For improvement of bhAAC expression in WB-12, we introduced DNA fragments encoding chimeric bhAACs, in which the N-terminal region of the bhAAC extending into the cytosol was replaced by the corresponding regions of the type 1 and type 2 yeast AAC isoforms (yAAC1 and yAAC2). These transformants grew well, and the amounts of the chimeric bhAACs in their mitochondria were as high as that of yAAC2. The carriers expressed showed essentially the same ADP transport activities as that of AAC in bovine heart mitochondria.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Biological Transport (日) (読) [継承]
4. (英) Cattle (日) (読) [継承]
5. (英) Mitochondria (日) (読) [継承]
6. (英) Mitochondria, Heart (日) (読) [継承]
7. (英) Mitochondrial ADP, ATP Translocases (日) (読) [継承]
8. (英) Molecular Sequence Data (日) (読) [継承]
9. (英) Mutation (日) (読) [継承]
10. (英) Oxidative Phosphorylation (日) (読) [継承]
11. (英) Plasmids (日) (読) [継承]
12. (英) Saccharomyces cerevisiae (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochimica et Biophysica Acta (BBA) - Bioenergetics ([Elsevier Science])
(pISSN: 0005-2728, eISSN: 1879-2650)

ISSN (任意): 0005-2728
ISSN: 0005-2728 (pISSN: 0005-2728, eISSN: 1879-2650)
Title: Biochimica et biophysica acta. Bioenergetics
Title(ISO): Biochim Biophys Acta Bioenerg
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 1409 [継承]
(必須): 3 [継承]
(必須): 113 124 [継承]
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年月日 (必須): 西暦 1999年 1月 5日 (平成 11年 1月 5日) [継承]
URL (任意):
DOI (任意): 10.1016/S0005-2728(98)00155-8    (→Scopusで検索) [継承]
PMID (任意): 9878703    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi-1, Tokushima 770-8505, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Mitsuru Hashimoto, Yasuo Shinohara, Eiji Majima, Takashi Hatanaka, Naoshi Yamazaki and Hiroshi Terada : Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers., Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol.1409, No.3, 113-124, 1999.
欧文冊子 ● Mitsuru Hashimoto, Yasuo Shinohara, Eiji Majima, Takashi Hatanaka, Naoshi Yamazaki and Hiroshi Terada : Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers., Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol.1409, No.3, 113-124, 1999.

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