『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=82894)

EID=82894EID:82894, Map:0, LastModified:2011年6月23日(木) 11:28:53, Operator:[佐藤 高則], Avail:TRUE, Censor:0, Owner:[佐藤 高則], Read:継承, Write:継承, Delete:継承.
種別 (必須): 国内講演発表 [継承]
言語 (必須): 日本語 [継承]
招待 (推奨):
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カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.総合科学部.自然システム学科 (1993年4月1日〜) [継承]
著者 (必須): 1.佐藤 高則
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学籍番号 (推奨):
[継承]
2. (英) Kohaku Yuko (日) (読)
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[継承]
3. (英) Sugiyama Noriko (日) (読)
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[継承]
4. (英) Yamaguchi Yasuaki (日) (読)
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[継承]
5. (英) Kouzai Miho (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Threonine 138 is crucial for Quaternary Structure and Thermostability of Thermus thermophilus Inorganic Pyrophosphatase.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Inorganic pyrophosphatase (E.C. 3.6.1.1) from Thermus thermophilus (Tth PPase) forms the hexamer, and it was suggested from X-ray studies that its intersubunit interactions stabilize the whole molecule. However, the contribution of Thr138 at intertrimer interface to quaternary structure and thermostability was unknown functionally. Therefore, we prepared the Thr138-substituted variants (T138A, V, N, and H) by site-directed mutagenesis. Then, the enzyme activities of T138A and V were drastically decreased to 15 and 30 % of the wild type respectively. In particular, half of T138A hexamer were dissociated into trimers even in native state, and their thermostabilities of enzyme activity and quaternary structure were decreased relative to those of the wild type Tth PPase. On the other hand, T138N and T138H variants maintained the enzyme activity and hexamer in the native state, which thermostabilities of hexamer were higher than those of T138A and V. Moreover, reassociation of hexamer in T138H and N variants was possible by increasing the enzyme concentration up to 1 mg/ml. Therefore, we suggest that both methyl and polar groups in Thr138 of Tth PPase are crucial for thermostability and quaternary structure, and it may contribute to the formation of stable trimer-trimer interface.  (日)    [継承]
キーワード (推奨):
発行所 (推奨): 日本生化学会 [継承]
誌名 (必須): 生化学 ([日本生化学会])
(resolved by 0037-1017)
ISSN: 0037-1017 (pISSN: 0037-1017, eISSN: 2189-0544)
Title: Seikagaku. The Journal of Japanese Biochemical Society
Title(ISO): Seikagaku
Publisher: Nihon Seikagakkai/Japanese Biochemical Society
 (NLM Catalog  (医中誌Web  (Scopus (Scopus information is found. [need login])
(resolved by 2189-0544)
ISSN: 0037-1017 (pISSN: 0037-1017, eISSN: 2189-0544)
Title: Seikagaku. The Journal of Japanese Biochemical Society
Title(ISO): Seikagaku
Publisher: Nihon Seikagakkai/Japanese Biochemical Society
 (NLM Catalog  (医中誌Web  (Scopus (Scopus information is found. [need login])

ISSN (任意):
[継承]
(必須): 76 [継承]
(必須): 8 [継承]
(必須): 809 809 [継承]
都市 (必須): 横浜 (Yokohama/[日本国]) [継承]
年月日 (必須): 西暦 2004年 10月 14日 (平成 16年 10月 14日) [継承]
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標準的な表示

和文冊子 ● 佐藤 高則, Yuko Kohaku, Noriko Sugiyama, Yasuaki Yamaguchi, Miho Kouzai : Threonine 138 is crucial for Quaternary Structure and Thermostability of Thermus thermophilus Inorganic Pyrophosphatase., 生化学, Vol.76, No.8, 809, 2004年10月.
欧文冊子 ● Takanori Satoh, Yuko Kohaku, Noriko Sugiyama, Yasuaki Yamaguchi and Miho Kouzai : Threonine 138 is crucial for Quaternary Structure and Thermostability of Thermus thermophilus Inorganic Pyrophosphatase., Seikagaku, Vol.76, No.8, 809, Oct. 2004.

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