『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=79650)

EID=79650EID:79650, Map:0, LastModified:2017年2月23日(木) 10:46:12, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[木戸 淳一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.木戸 淳一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Nishikawa S. (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Ishida H. (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.山下 菊治
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.北村 清一郎
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Kohri K. (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7.永田 俊彦
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Identification of calprotein , a calcium binding leukocyte protein, In human dental calculus matrix  (日)    [継承]
副題 (任意):
要約 (任意): (英) Calprotectin is a calcium binding protein produced by leukocytes, macrophages and epithelial cells, and its levels in several tissues increase during infections and in many inflamed areas, suggesting that it may be an indicator of inflammatory activity. Osteopontin is a prominent phosphorylated glycoprotein in bone matrix, having calcium binding capacity. Recently, it has been reported that calprotectin and osteopontin are present in urinary stones (pathological mineralized masses in the body), and that these proteins may be involved in their formation. Dental calculus formed by mineralization of dental plaque is an inflammatory factor which may contribute to periodontal disease. It contains many organic components involved in mineralization. We recently found osteopontin molecules in human dental calculus and suggested that the components of its matrix may be similar to those of urinary stones. In this study, we investigated the presence of calprotectin in human dental calculus by immunohistochemical and immunoblotting analyses using a specific antibody for calprotectin. After fixation and demineralization of dental calculi adhered to tooth roots, sections embedded in paraffin were immunoreacted with the antibody for calprotectin and positive immunostaining for calprotectin was observed. Dental calculus proteins were then extracted with EDTA and separated by electrophoresis on 15% polyacrylamide gels. By immunoblotting analysis, 3 or 4 bands were observed at 11, 14.5, 22-25, 28 or 36.5 kDa and these patterns corresponded to those of calprotectin subunits. When non-immune rabbit serum was used instead of calprotectin-specific antibody as a negative control, no immunoreactivity was observed. These findings indicate that calprotectin is associated not only with antibacterial action but also with calcium binding capacity during dental calculus formation.  (日)    [継承]
キーワード (推奨): 1. (英) Calcium-Binding Proteins (日) (読) [継承]
2. (英) Decalcification Technique (日) (読) [継承]
3. (英) Dental Calculus (日) (読) [継承]
4. (英) Electrophoresis, Polyacrylamide Gel (日) (読) [継承]
5. (英) Humans (日) (読) [継承]
6. (英) Immunoblotting (日) (読) [継承]
7.免疫組織化学 (immunohistochemistry) [継承]
8. (英) Leukocyte L1 Antigen Complex (日) (読) [継承]
9. (英) Neural Cell Adhesion Molecules (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Periodontal Research ([Blackwell Publishing Ltd])
(pISSN: 0022-3484, eISSN: 1600-0765)

ISSN (任意): 0022-3484
ISSN: 0022-3484 (pISSN: 0022-3484, eISSN: 1600-0765)
Title: Journal of periodontal research
Title(ISO): J Periodontal Res
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 32 [継承]
(必須): 4 [継承]
(必須): 335 361 [継承]
都市 (任意):
年月日 (必須): 西暦 1997年 5月 初日 (平成 9年 5月 初日) [継承]
URL (任意):
DOI (任意): 10.1111/j.1600-0765.1997.tb00545.x    (→Scopusで検索) [継承]
PMID (任意): 9210089    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Jun-ichi Kido, S. Nishikawa, H. Ishida, Kikuji Yamashita, Seiichiro Kitamura, K. Kohri and Toshihiko Nagata : Identification of calprotein , a calcium binding leukocyte protein, In human dental calculus matrix, Journal of Periodontal Research, Vol.32, No.4, 335-361, 1997.
欧文冊子 ● Jun-ichi Kido, S. Nishikawa, H. Ishida, Kikuji Yamashita, Seiichiro Kitamura, K. Kohri and Toshihiko Nagata : Identification of calprotein , a calcium binding leukocyte protein, In human dental calculus matrix, Journal of Periodontal Research, Vol.32, No.4, 335-361, 1997.

関連情報

Number of session users = 1, LA = 0.52, Max(EID) = 383329, Max(EOID) = 1024603.