『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=74185EID:74185, Map:0, LastModified:2013年11月14日(木) 08:59:56, Operator:[佐藤 高則], Avail:TRUE, Censor:0, Owner:[佐藤 高則], Read:継承, Write:継承, Delete:継承.
種別 (必須): 国内講演発表 [継承]
言語 (必須):
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.総合科学部.自然システム学科 (1993年4月1日〜) [継承]
著者 (必須): 1.佐藤 高則
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Miyano-Ono Momoyo (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Yamaguchi Yasuaki (日) (読)
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
4.瀨尾 クニ子
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
5.秋吉 博登
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Exploration of proteins contributing to the amitrole-resistance in Escherichia coli  (日)    [継承]
副題 (任意):
要約 (任意): (英) Amitrole is a kind of environmental hormones and inhibits the bacterial growth. It was reported that the chemical mutagen treated Escherichia coli (E.coli) cells exhibit the resistance against amitrole. However, much information on the mechanism and proteins contributing to this resistance were not shown. Therefore, in order to elucidate the mechanism on the amitrole resistance in E.coli, we prepared the E.coli JM109 mutant strain by treating with MNNG, and then selected the amitrole-resistant E. coli strain. The obtained mutant strain was possible for proliferating in the M9 minimal medium containing 2 mg/ml amitrole (AT2 medium). Therefore, we examined the expressed proteins in mutant strain cells cultured under the various conditions. As result, it was suggested the expression of 22kDa protein (P1) was suppressed in the M9 minimal medium containing amitrole. Amino acid sequence analysis of P1 revealed that it might be alkyl hydroperoxide reductase C22 protein. On the other hand, we found the expression of the other protein (P2) was increased when cultured in AT2 medium. Furthermore, we explored the amitrole binding proteins in E.coli mutant cells by amitrole affinity chromatography. And then, five proteins (P3-P7) were selected as candidates of amitrole binding proteins. Further investigations about these proteins (P2-P7) are now undertaken.  (日)    [継承]
キーワード (推奨):
発行所 (推奨): 日本生化学会 [継承]
誌名 (必須): 生化学 ([日本生化学会])
(resolved by 0037-1017)
ISSN: 0037-1017 (pISSN: 0037-1017, eISSN: 2189-0544)
Title: Seikagaku. The Journal of Japanese Biochemical Society
Title(ISO): Seikagaku
Publisher: Nihon Seikagakkai/Japanese Biochemical Society
 (NLM Catalog  (医中誌Web  (Scopus (Scopus information is found. [need login])
(resolved by 2189-0544)
ISSN: 0037-1017 (pISSN: 0037-1017, eISSN: 2189-0544)
Title: Seikagaku. The Journal of Japanese Biochemical Society
Title(ISO): Seikagaku
Publisher: Nihon Seikagakkai/Japanese Biochemical Society
 (NLM Catalog  (医中誌Web  (Scopus (Scopus information is found. [need login])

ISSN (任意):
[継承]
(必須): 75 [継承]
(必須): 8 [継承]
(必須): 957 957 [継承]
都市 (必須): 横浜 (Yokohama/[日本国]) [継承]
年月日 (必須): 西暦 2003年 8月 10日 (平成 15年 8月 10日) [継承]
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標準的な表示

和文冊子 ● Takanori Satoh, Momoyo Miyano-Ono, Yasuaki Yamaguchi, Kuniko Seo and Hiroto Akiyoshi : Exploration of proteins contributing to the amitrole-resistance in Escherichia coli, Seikagaku, Vol.75, No.8, 957, Aug. 2003.
欧文冊子 ● Takanori Satoh, Momoyo Miyano-Ono, Yasuaki Yamaguchi, Kuniko Seo and Hiroto Akiyoshi : Exploration of proteins contributing to the amitrole-resistance in Escherichia coli, Seikagaku, Vol.75, No.8, 957, Aug. 2003.

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