『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hino Satoshi (日) (読)
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2. (英) Kawamata Hitoshi (日) (読)
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3.表原 文江
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4.内田 大亮
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5. (英) Begum NM (日) (読)
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6.吉田 秀夫
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7.佐藤 光信
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題名 (必須): (英) Leucine zipper structure of TSC-22 (TGF-b stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells  (日)    [継承]
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要約 (任意): (英) Several investigators have demonstrated that TGF-beta stimulated clone-22 (TSC-22) regulates cell growth and differentiation, and cell death. TSC-22 is a putative transcriptional regulator containing a leucine zipper-like structure and a nuclear export signal. We previously showed the cytoplasmic localization of TSC-22 and the nuclear translocation of TSC-22 concomitant with induction of apoptosis in salivary gland cancer cells. In the present study, we attempted to identify the active domain of TSC-22 protein that regulated the biological functions of TSC-22. We constructed three mammalian expression vectors, which could produce full length TSC-22 only in cytoplasm, the leucine zipper structure of TSC-22 in both cytoplasm and nucleus, and the leucine zipper structure only in nucleus. Then, we transfected a salivary gland cancer cell line, HSG with these expression vectors, and investigated the growth profile of the transfectants. None of the TSC-22 constructs inhibited the monolayer growth and the anchorage-dependent colony formation of HSG cells. However, the leucine zipper structure of TSC-22 markedly inhibited the anchorage-independent colony formation of HSG cells (p<0.001; one way ANOVA). Full length TSC-22 also suppressed the anchorage-independent colony formation of HSG cells, although the effect of full length TSC-22 was much lower than those of the leucine zipper constructs. These observations suggest that the leucine zipper structure in TSC-22 protein is an active domain that negatively regulates the growth of salivary gland cancer cells.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Motifs (日) (読) [継承]
2. (英) Cell Adhesion (日) (読) [継承]
3. (英) Colony-Forming Units Assay (日) (読) [継承]
4. (英) DNA Primers (日) (読) [継承]
5. (英) Green Fluorescent Proteins (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7. (英) Leucine Zippers (日) (読) [継承]
8. (英) Luminescent Proteins (日) (読) [継承]
9. (英) Nuclear Localization Signals (日) (読) [継承]
10. (英) Plasmids (日) (読) [継承]
11. (英) Recombinant Fusion Proteins (日) (読) [継承]
12. (英) Repressor Proteins (日) (読) [継承]
13. (英) Salivary Gland Neoplasms (日) (読) [継承]
14. (英) Transfection (日) (読) [継承]
15. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Oncology Reports (Ethnikon Hidryma Ereunōn (Greece))
(pISSN: 1021-335X, eISSN: 1791-2431)

ISSN (任意): 1021-335X
ISSN: 1021-335X (pISSN: 1021-335X, eISSN: 1791-2431)
Title: Oncology reports
Title(ISO): Oncol. Rep.
Publisher: Spandidos Publications
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 9 [継承]
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(必須): 371 374 [継承]
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年月日 (必須): 西暦 2002年 4月 初日 (平成 14年 4月 初日) [継承]
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PMID (任意): 11836610    (→Scopusで検索) [継承]
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WOS (任意): 000173889500027 [継承]
Scopus (任意): 2-s2.0-0036511210 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) MedlineDate: 2002 Mar-Apr  (日)    [継承]

標準的な表示

和文冊子 ● Satoshi Hino, Hitoshi Kawamata, Fumie Omotehara, Daisuke Uchida, NM Begum, Hideo Yoshida and Mitsunobu Sato : Leucine zipper structure of TSC-22 (TGF-b stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells, Oncology Reports, Vol.9, No.2, 371-374, 2002.
欧文冊子 ● Satoshi Hino, Hitoshi Kawamata, Fumie Omotehara, Daisuke Uchida, NM Begum, Hideo Yoshida and Mitsunobu Sato : Leucine zipper structure of TSC-22 (TGF-b stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells, Oncology Reports, Vol.9, No.2, 371-374, 2002.

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