『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1. (英) Morimoto Yasuhiro (日) (読)
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[継承]
2.森本 景之
役割 (任意): 共著 [継承]
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[継承]
3. (英) Kobayashi Shigeru (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Ohba Takeshi (日) (読)
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学籍番号 (推奨):
[継承]
5.羽地 達次
役割 (任意): 共著 [継承]
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[継承]
題名 (必須): (英) The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland cell line HSG cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) To investigate a possible relationship between protein phosphorylation or dephosphorylation status and apoptosis in salivary gland cells, we examined the effects of okadaic acid and calyculin A, the protein phosphatase inhibitors, on cultured human submandibular gland ductal cell line, HSG cells. HSG cells at subconfluent stages were exposed to varying concentrations of okadaic acid or calyculin A. Apoptoses were analysed in HSG cells by phase-contrast microscopy, WST-1 cytotoxicity assay, Hoechst 33342 staining, and DNA ladder formation. Both okadaic acid and calyculin A induced cell death in HSG cells in a dose-dependent fashion. Marked nuclear condensation and fragmentation of chromatin was observed in HSG cells. DNA ladder formation was also detected in HSG cells by treatment with okadaic acid or calyculin A. The induced DNA ladder formation was dose-dependent with maximal effect at concentrations of 50 nM okadaic acid and 2 nM calyculin A, respectively, and were time-dependent from 14 h to 48 h. To further determine if new gene transcription and protein synthesis regulate okadaic acid-induced apoptosis in HSG cells, the cells were treated with cycloheximide or actinomycin D in the presence of 20 nM okadaic acid. Neither inhibitor protected the cells against okadaic acid-induced apoptosis. Based on the known selectivity of okadaic acid and calyculin A, our results indicate that the pathway of the apoptosis in the cultured salivary gland cells is regulated by protein phosphatase type 1 or type 2A. Our results also suggest that new protein synthesis and/or mRNA expression are not involved in okadaic acid-induced apoptosis in HSG cells.  (日)    [継承]
キーワード (推奨): 1.アポトーシス (apoptosis) [継承]
2. (英) Cells, Cultured (日) (読) [継承]
3. (英) Cycloheximide (日) (読) [継承]
4. (英) DNA Fragmentation (日) (読) [継承]
5. (英) Dactinomycin (日) (読) [継承]
6. (英) Enzyme Inhibitors (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Okadaic Acid (日) (読) [継承]
9. (英) Oxazoles (日) (読) [継承]
10. (英) Phosphoprotein Phosphatases (日) (読) [継承]
11.リン酸化 (phosphorylation) [継承]
12. (英) Protein Synthesis Inhibitors (日) (読) [継承]
13. (英) Salivary Ducts (日) (読) [継承]
14. (英) Submandibular Gland (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Oral Diseases (Munksgaard)
(pISSN: 1354-523X, eISSN: 1601-0825)

ISSN (任意): 1354-523X
ISSN: 1354-523X (pISSN: 1354-523X, eISSN: 1601-0825)
Title: Oral diseases
Title(ISO): Oral Dis
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 1999年 1月 初日 (平成 11年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1111/j.1601-0825.1999.tb00073.x    (→Scopusで検索) [継承]
PMID (任意): 10522205    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yasuhiro Morimoto, Hiroyuki Morimoto, Shigeru Kobayashi, Takeshi Ohba and Tatsuji Haneji : The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland cell line HSG cells., Oral Diseases, Vol.5, No.2, 104-110, 1999.
欧文冊子 ● Yasuhiro Morimoto, Hiroyuki Morimoto, Shigeru Kobayashi, Takeshi Ohba and Tatsuji Haneji : The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland cell line HSG cells., Oral Diseases, Vol.5, No.2, 104-110, 1999.

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