『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=71601EID:71601, Map:0, LastModified:2017年7月3日(月) 14:49:11, Operator:[三好 小文], Avail:TRUE, Censor:0, Owner:[[学部長]/[徳島大学.歯学部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨): 1.東京医科歯科大学.生体材料工学研究所 [継承]
2.科学技術振興事業団 (1996年10月1日〜2003年9月30日) [継承]
著者 (必須): 1. (英) Fujimoto Kazuhisa (日) 藤本 和久 (読) ふじもと かずひさ
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学籍番号 (推奨):
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2.飯田 博一
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3. (英) Kawakami Masako (日) 川上 雅子 (読) かわかみ まさこ
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4. (英) Bando Toshikazu (日) 板東 俊和 (読) ばんどう としかず
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5. (英) Tao Zhi-Fu (日) (読)
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6. (英) Sugiyama Hiroshi (日) 杉山 弘 (読) すぎやま ひろし
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題名 (必須): (英) Sequence-specific protection of plasmid DNA from restriction endonuclease hydrolysis by pyrrole-imidazole-cyclopropapyrroloindole conjugates  (日)    [継承]
副題 (任意):
要約 (任意): (英) The pyrrole-imidazole (Py-Im) triamide-cyclopropa pyrroloindole (CPI) conjugates ImPyImLDu86 (7) and ImImPyLDu86 (14) were synthesized and their alkylating activities and inhibitory effects on DNA hydrolysis by restriction endonucleases were examined. Sequencing gel analysis demonstrated that conjugates 7 and 14 specifically alkylated DNA at 5'-CGCGCG-3' and 5'-PyGGCCPu-3', respectively. Agarose gel electrophoresis indicated that incubation of a supercoiled plasmid, pSPORT I (4109 bp), with conjugate 7 effectively inhibited its hydrolysis by BssHII (5'-G_CGCGC-3'), whereas conjugate 14 had no effect on this hydrolysis. These results suggest that conjugate 7 sequence-specifically inhibits the hydrolysis of DNA by BssHII. Sequence-specific alkylation by the Py-Im triamide-CPI conjugates was further confirmed by inhibition of the Eco52I (5'-C_GGCCG-3') hydrolysis of conjugate 14-treated pQBI PGK (5387 bp). In clear contrast, hydrolysis of pQB1 PGK by DraI (3'-TTT_AAA-3') was not inhibited by 5 micro M conjugate 14. That ImImPy did not inhibit the hydrolysis of pQB1 PGK indicates that covalent bond formation is necessary for inhibition. A similar experiment, using linear pQBI PGK, achieved the same extent of protection of the DNA with approximately half the concentration of conjugate 14 as was required to protect supercoiled DNA from hydrolysis.  (日)    [継承]
キーワード (推奨): 1. (英) Alkylation (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) DNA (日) (読) [継承]
4. (英) DNA Restriction Enzymes (日) (読) [継承]
5. (英) DNA, Superhelical (日) (読) [継承]
6. (英) Deoxyribonucleases, Type II Site-Specific (日) (読) [継承]
7. (英) Electrophoresis, Polyacrylamide Gel (日) (読) [継承]
8. (英) Imidazoles (日) (読) [継承]
9. (英) Plasmids (日) (読) [継承]
10. (英) Pyrroles (日) (読) [継承]
11. (英) Sequence Analysis, DNA (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Nucleic Acids Research ([Oxford University Press])
(pISSN: 0305-1048, eISSN: 1362-4962)

ISSN (任意): 1362-4962
ISSN: 0305-1048 (pISSN: 0305-1048, eISSN: 1362-4962)
Title: Nucleic acids research
Title(ISO): Nucleic Acids Res
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 30 [継承]
(必須): 17 [継承]
(必須): 3748 3753 [継承]
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年月日 (必須): 西暦 2002年 9月 1日 (平成 14年 9月 1日) [継承]
URL (任意):
DOI (任意): 10.1093/nar/gkf486    (→Scopusで検索) [継承]
PMID (任意): 12202760    (→Scopusで検索) [継承]
CRID (任意):
NAID (任意):
WOS (任意): 000177998100017 [継承]
Scopus (任意): 2-s2.0-0036712067 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) OtherID: PMC137410  (日)    [継承]

標準的な表示

和文冊子 ● Kazuhisa Fujimoto, Hirokazu Iida, Masako Kawakami, Toshikazu Bando, Zhi-Fu Tao and Hiroshi Sugiyama : Sequence-specific protection of plasmid DNA from restriction endonuclease hydrolysis by pyrrole-imidazole-cyclopropapyrroloindole conjugates, Nucleic Acids Research, Vol.30, No.17, 3748-3753, 2002.
欧文冊子 ● Kazuhisa Fujimoto, Hirokazu Iida, Masako Kawakami, Toshikazu Bando, Zhi-Fu Tao and Hiroshi Sugiyama : Sequence-specific protection of plasmid DNA from restriction endonuclease hydrolysis by pyrrole-imidazole-cyclopropapyrroloindole conjugates, Nucleic Acids Research, Vol.30, No.17, 3748-3753, 2002.

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