『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.歯学部.歯学科.基礎口腔科学講座.予防歯学 (予防歯科学講座) [継承]
著者 (必須): 1.日野出 大輔 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健衛生学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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学籍番号 (推奨):
[継承]
2.横山 正明
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貢献度 (任意):
学籍番号 (推奨):
[継承]
3.田部 慎一
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貢献度 (任意):
学籍番号 (推奨):
[継承]
4.吉岡 昌美
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[継承]
5.中村 亮 ([徳島大学])
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Antigenic properties of the GroEL-like protein of Campylobacter rectus  (日)    [継承]
副題 (任意):
要約 (任意): (英) The purpose of this study was to clarify the antigenic properties of the GroEL-like protein of Campylobacter rectus using a specific polyclonal antibody directed to the purified 64-kDa GroEL-like protein (pAb-CrGroEL), a polyclonal antibody directed to the Actinobacillus actinomycetemcomitans GroEL-like protein (pAb-AaGroEL) and a monoclonal antibody against the recombinant human HSP60 (mAb-HuHSP60). In SDS-PAGE/Western immunoblotting analysis, mAb-HuHSP60, pAb-CrGroEL and pAb-AaGroEL were found to react with the GroEL-like protein (64-kDa) present in all C. rectus strains. A 150-kDa protein in C. rectus ATCC 33238 also reacted strongly with pAb-CrGroEL. This 150-kDa protein was found to be present on the surface-associated material of bacterial cells, as determined by transmission electron microscopy and immunogold labelling of cells with pAb-CrGroEL. Analysis of the first 20 N-terminal amino acids of the sequence of the 150-kDa protein revealed a strong homology (80%) with the C. rectus surface layer (S-layer) protein. Investigation of the biochemical nature of antigenic determinants using periodic acid and proteolytic enzymes showed that the C. rectus GroEL-like protein possessed immunodominant epitopes in both peptide and carbohydrate chains, and that the immunoreactive determinants of the 150-kDa protein belonged to carbohydrate. These results suggest that the GroEL-like protein and the S-layer protein of C. rectus may share the same carbohydrate epitopes.  (日)    [継承]
キーワード (推奨): 1. (英) Actinobacillus actinomycetemcomitans (日) (読) [継承]
2. (英) Antigens, Bacterial (日) (読) [継承]
3. (英) Bacterial Outer Membrane Proteins (日) (読) [継承]
4. (英) Bacterial Proteins (日) (読) [継承]
5. (英) Blotting, Western (日) (読) [継承]
6. (英) Campylobacter (日) (読) [継承]
7. (英) Chaperonin 60 (日) (読) [継承]
8. (英) Epitopes (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10. (英) Membrane Glycoproteins (日) (読) [継承]
11. (英) Sequence Homology, Amino Acid (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Oral Microbiology and Immunology ([John Wiley & Sons, Inc.])
(pISSN: 0902-0055, eISSN: 1399-302X)

ISSN (任意): 0902-0055
ISSN: 0902-0055 (pISSN: 0902-0055, eISSN: 1399-302X)
Title: Oral microbiology and immunology
Title(ISO): Oral Microbiol. Immunol.
Publisher: Blackwell
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 17 [継承]
(必須): 1 [継承]
(必須): 16 21 [継承]
都市 (任意):
年月日 (必須): 西暦 2002年 2月 初日 (平成 14年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.1046/j.0902-0055.2001.00086.x    (→Scopusで検索) [継承]
PMID (任意): 11860551    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Preventive Dentistry, School of Dentistry, The University of Tokushima, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Daisuke Hinode, Masaaki Yokoyama, Shin-ichi Tanabe, Masami Yoshioka and Ryo Nakamura : Antigenic properties of the GroEL-like protein of Campylobacter rectus, Oral Microbiology and Immunology, Vol.17, No.1, 16-21, 2002.
欧文冊子 ● Daisuke Hinode, Masaaki Yokoyama, Shin-ichi Tanabe, Masami Yoshioka and Ryo Nakamura : Antigenic properties of the GroEL-like protein of Campylobacter rectus, Oral Microbiology and Immunology, Vol.17, No.1, 16-21, 2002.

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