『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨): □Peer Review [継承]
○カテゴリ (推奨): □研究 [継承]
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
○著者 (必須): 1. (英) Morimoto Yasuhiro (日) (読)
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2.森本 景之
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3.岡村 裕彦
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[継承]
4. (英) Nomiyama Kimiko (日) (読)
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[継承]
5. (英) Nakamuta Nobuaki (日) (読)
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6. (英) Kobayashi Shigeru (日) (読)
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7. (英) Kito Shinji (日) (読)
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8. (英) Ohba Takeshi (日) (読)
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9.羽地 達次
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○題名 (必須): □(英) Upregulation of the expression of Fas antigen and Fas ligand in a human submandibular gland ductal cell line by okadaic acid.  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) Fas receptor is a member of a superfamily of receptors characterized by cysteine-rich motifs in the extracellular domain of the molecule. Binding of Fas ligand to the receptor leads to receptor activation and the induction of intracellular signals that result in apoptotic cell death. In the present study, the expression of mRNA and proteins of Fas receptor and Fas ligand were examined in human submandibular gland ductal (HSG) cells treated with okadaic acid by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analysis. Six hundred and eighty-two bp of the PCR product of Fas receptor mRNA was detected in HSG cells and a protein with an estimated molecular weight of 58,000 was expressed in HSG cells. Treatment of HSG cells with an agonistic anti-Fas monoclonal antibody resulted in death of HSG cells, indicating that the functional Fas receptor protein is expressed in HSG cells. Fas receptor protein expression stimulated by okadaic acid was elevated in a dose- and time-dependent manner, with maximal expression at 20 nM and 48 h treatment. Fas ligand mRNA was also detected constitutively in HSG cells by RT-PCR. Okadaic acid stimulated the expression of Fas ligand protein in HSG cells in a time-dependent manner, while the expression of the ligand was low in untreated HSG cells. The molecular weight of Fas ligand was estimated as 68,000. An antagonistic anti-Fas ligand monoclonal antibody prevented okadaic acid-induced death in HSG cells in a dose-dependent fashion as determined by WST-1 assay. The results indicate that the expression of Fas receptor and ligand is regulated by protein phosphatase(s) sensitive to okadaic acid and is involved in okadaic acid-induced apoptosis in HSG cells. The results also suggest that the Fas receptor-ligand system might regulate apoptosis in HSG cells.  (日)    [継承]
○キーワード (推奨): 1. (英) Antibodies, Monoclonal (日) (読) [継承]
2. (英) Antigens, CD95 (日) (読) [継承]
3. (英) Antigens, Surface (日) (読) [継承]
4.アポトーシス (apoptosis) [継承]
5. (英) Base Pairing (日) (読) [継承]
6. (英) Cell Line (日) (読) [継承]
7. (英) Dose-Response Relationship, Drug (日) (読) [継承]
8. (英) Enzyme Inhibitors (日) (読) [継承]
9. (英) Fas Ligand Protein (日) (読) [継承]
10. (英) Gene Expression Regulation (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Immunoblotting (日) (読) [継承]
13. (英) Ligands (日) (読) [継承]
14. (英) Membrane Glycoproteins (日) (読) [継承]
15.分子量 (molecular weight) [継承]
16. (英) Okadaic Acid (日) (読) [継承]
17. (英) Phosphoprotein Phosphatases (日) (読) [継承]
18. (英) Polymerase Chain Reaction (日) (読) [継承]
19. (英) RNA, Messenger (日) (読) [継承]
20. (英) Salivary Ducts (日) (読) [継承]
21.シグナル伝達 (signal transduction) [継承]
22. (英) Submandibular Gland (日) (読) [継承]
23. (英) Time Factors (日) (読) [継承]
24. (英) Up-Regulation (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □Archives of Oral Biology ([Elsevier])
(pISSN: 0003-9969, eISSN: 1879-1506)

○ISSN (任意): □0003-9969
ISSN: 0003-9969 (pISSN: 0003-9969, eISSN: 1879-1506)
Title: Archives of oral biology
Title(ISO): Arch Oral Biol
Publisher: Elsevier Ltd
 (NLM Catalog)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □45 [継承]
○号 (必須): □8 [継承]
○頁 (必須): □657 666 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2000年 1月 初日 (平成 12年 1月 初日) [継承]
○URL (任意):
○DOI (任意): □10.1016/S0003-9969(00)00038-8    (→Scopusで検索) [継承]
○PMID (任意): □10869477    (→Scopusで検索) [継承]
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○備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yasuhiro Morimoto, Hiroyuki Morimoto, Hirohiko Okamura, Kimiko Nomiyama, Nobuaki Nakamuta, Shigeru Kobayashi, Shinji Kito, Takeshi Ohba and Tatsuji Haneji : Upregulation of the expression of Fas antigen and Fas ligand in a human submandibular gland ductal cell line by okadaic acid., Archives of Oral Biology, Vol.45, No.8, 657-666, 2000.
欧文冊子 ● Yasuhiro Morimoto, Hiroyuki Morimoto, Hirohiko Okamura, Kimiko Nomiyama, Nobuaki Nakamuta, Shigeru Kobayashi, Shinji Kito, Takeshi Ohba and Tatsuji Haneji : Upregulation of the expression of Fas antigen and Fas ligand in a human submandibular gland ductal cell line by okadaic acid., Archives of Oral Biology, Vol.45, No.8, 657-666, 2000.

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