『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨): □Peer Review [継承]
○カテゴリ (推奨): □研究 [継承]
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
○著者 (必須): 1. (英) Seta Chihiro (日) (読)
○役割 (任意):
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○学籍番号 (推奨):
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2. (英) Fujita Michi (日) (読)
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○学籍番号 (推奨):
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3. (英) Muraki Yukoh (日) (読)
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○学籍番号 (推奨):
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4. (英) Fukuda JInichi (日) (読)
○役割 (任意):
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○学籍番号 (推奨):
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5. (英) Kobayashi Shigeru (日) (読)
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○学籍番号 (推奨):
[継承]
6.羽地 達次
○役割 (任意): □共著 [継承]
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○学籍番号 (推奨):
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○題名 (必須): □(英) Fas expression and Fas monoclonal antibody-induced apoptosis in human squamous cell carcinoma cell line SCC-25 cells.  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) Fas antigen is a cell surface protein that mediates apoptosis via signal transduction from the plasma membrane. Using reverse transcriptase-polymerase chain reaction (RT-PCR), messenger RNA for Fas antigen was detected in the human oral squamous cell carcinoma cell line, SCC-25. In serum-free medium, a monoclonal anti-Fas antibody (CH-11) induced Fas antigen expression in SCC-25 cells, as determined by immunocytochemistry and Western blotting, using an anti-Fas polyclonal antibody (Fas D) as primary antibody. Fas antigen was localized to the cytoplasm and the cell membrane. The molecular weight of the protein recognized by Western blot analysis was 35,000, consistent with the value reported for the Fas antigen. The CH-11 antibody did not induce Fas antigen expression in serum-containing medium. To determine whether CH-11 could induce apoptosis in oral squamous cell carcinoma, we examined its effects on the survival of cultured SCC-25 cells. Anti-Fas monoclonal antibody in serum-free medium induced cytotoxicity in SCC-25 cells in a time-dependent manner up to 8 h, as determined by phase-contrast microscopy and WST-1 assay. Marked nuclear condensation and fragmentation of chromatin were observed in the CH-11-treated cells using Hoechst 33342 staining. This anti-Fas monoclonal antibody also induced DNA ladder formation in SCC-25 cells in a time-dependent manner. The present results indicate that the anti-Fas monoclonal antibody (CH-11) may mediate apoptosis by binding to the Fas antigen expressed in SCC-25 cells.  (日)    [継承]
○キーワード (推奨): 1. (英) Antibodies, Monoclonal (日) (読) [継承]
2. (英) Antigens, CD95 (日) (読) [継承]
3.アポトーシス (apoptosis) [継承]
4. (英) Carcinoma, Squamous Cell (日) (読) [継承]
5. (英) Cell Membrane (日) (読) [継承]
6. (英) Cell Nucleus (日) (読) [継承]
7. (英) Cell Survival (日) (読) [継承]
8. (英) Chromatin (日) (読) [継承]
9. (英) Culture Media, Serum-Free (日) (読) [継承]
10. (英) Cytoplasm (日) (読) [継承]
11. (英) Cytotoxicity, Immunologic (日) (読) [継承]
12. (英) DNA Fragmentation (日) (読) [継承]
13. (英) Gene Expression Regulation, Neoplastic (日) (読) [継承]
14. (英) Humans (日) (読) [継承]
15.分子量 (molecular weight) [継承]
16. (英) Mouth Neoplasms (日) (読) [継承]
17.シグナル伝達 (signal transduction) [継承]
18. (英) Time Factors (日) (読) [継承]
19. (英) Tumor Cells, Cultured (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □Journal of Oral Pathology & Medicine (International Association of Oral Pathologists/American Academy of Oral Pathology)
(pISSN: 0904-2512, eISSN: 1600-0714)

○ISSN (任意): □0904-2512
ISSN: 0904-2512 (pISSN: 0904-2512, eISSN: 1600-0714)
Title: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Title(ISO): J Oral Pathol Med
Supplier: International Association of Oral Pathologists
Publisher: Blackwell
 (NLM Catalog)   (Wiley)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □29 [継承]
○号 (必須): □6 [継承]
○頁 (必須): □271 278 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2000年 1月 初日 (平成 12年 1月 初日) [継承]
○URL (任意):
○DOI (任意): □10.1034/j.1600-0714.2000.290606.x    (→Scopusで検索) [継承]
○PMID (任意): □10890558    (→Scopusで検索) [継承]
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○Scopus (任意): □2-s2.0-0034088280 [継承]
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○備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Chihiro Seta, Michi Fujita, Yukoh Muraki, JInichi Fukuda, Shigeru Kobayashi and Tatsuji Haneji : Fas expression and Fas monoclonal antibody-induced apoptosis in human squamous cell carcinoma cell line SCC-25 cells., Journal of Oral Pathology & Medicine, Vol.29, No.6, 271-278, 2000.
欧文冊子 ● Chihiro Seta, Michi Fujita, Yukoh Muraki, JInichi Fukuda, Shigeru Kobayashi and Tatsuji Haneji : Fas expression and Fas monoclonal antibody-induced apoptosis in human squamous cell carcinoma cell line SCC-25 cells., Journal of Oral Pathology & Medicine, Vol.29, No.6, 271-278, 2000.

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