『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1. (英) Nakamura Koji (日) (読)
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[継承]
2. (英) Shima Hiroshi (日) (読)
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[継承]
3. (英) Watanabe Masahiko (日) (読)
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[継承]
4.羽地 達次
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[継承]
5. (英) Kikuchi Kunimi (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Molecular cloning and characterization of a novel dual specificity protein phosphatase possibly involved in spermatogenesis.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Dual-specificity protein phosphatases (DSPs) play roles in the regulation of mitogenic signal transduction for extracellular stimulation and the cell cycle. In the present study, we identified a novel DSP, termed TMDP (testis- and skeletal-muscle-specific DSP). Nucleotide sequence analysis of TMDP cDNA indicated that the open reading frame of 597 bp encodes a protein of 198 amino acid residues with a predicted molecular mass of 22.5 kDa. The deduced amino acid sequence contains a motif for a conserved catalytic domain of DSPs and shows highest similarity to human Vaccinia HI-related phosphatase (45.5% identity) but low homology to the mitogen-activated protein kinase phosphatase and CDC25 subfamilies of DSPs. Recombinant TMDP protein exhibited intrinsic phosphatase activity towards both phospho-seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Northern-blot analysis revealed that TMDP is most abundantly expressed in the testis. The expression in the testis is characterized as follows: (i) TMDP mRNA first appeared 3 weeks after birth, corresponding to the time that meiosis begins; (ii) TMDP mRNA was abundant in fractionated spermatocytes and round spermatids; and (iii) hybridization in situ showed that the TMDP mRNA is localized in spermatocytes and/or spermatids in seminiferous tubules. These data demonstrate that TMDP is a novel DSP abundantly expressed in the testis and suggest that TMDP may be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Base Sequence (日) (読) [継承]
4. (英) Cloning, Molecular (日) (読) [継承]
5. (英) Dual-Specificity Phosphatases (日) (読) [継承]
6. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
7. (英) In Situ Hybridization (日) (読) [継承]
8. (英) Male (日) (読) [継承]
9. (英) Mice (日) (読) [継承]
10. (英) Molecular Sequence Data (日) (読) [継承]
11. (英) Phosphoprotein Phosphatases (日) (読) [継承]
12. (英) Protein Tyrosine Phosphatases (日) (読) [継承]
13. (英) RNA, Messenger (日) (読) [継承]
14. (英) Recombinant Proteins (日) (読) [継承]
15. (英) Spermatocytes (日) (読) [継承]
16. (英) Spermatogenesis (日) (読) [継承]
17. (英) Substrate Specificity (日) (読) [継承]
18. (英) Testis (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 344 [継承]
(必須): 3 [継承]
(必須): 819 825 [継承]
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年月日 (必須): 西暦 1999年 1月 初日 (平成 11年 1月 初日) [継承]
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DOI (任意): 10.1042/bj3440819    (→Scopusで検索) [継承]
PMID (任意): 10585869    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.DataBankList.DataBank.DataBankName: GENBANK  (日)    [継承]
2.(英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB027003  (日)    [継承]
3.(英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB027004  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
5.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
6.(英) OtherID: PMC1220704  (日)    [継承]

標準的な表示

和文冊子 ● Koji Nakamura, Hiroshi Shima, Masahiko Watanabe, Tatsuji Haneji and Kunimi Kikuchi : Molecular cloning and characterization of a novel dual specificity protein phosphatase possibly involved in spermatogenesis., The Biochemical Journal, Vol.344, No.3, 819-825, 1999.
欧文冊子 ● Koji Nakamura, Hiroshi Shima, Masahiko Watanabe, Tatsuji Haneji and Kunimi Kikuchi : Molecular cloning and characterization of a novel dual specificity protein phosphatase possibly involved in spermatogenesis., The Biochemical Journal, Vol.344, No.3, 819-825, 1999.

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