| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日)
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| ○著者 (必須): | 1. | (英) Nakamura Koji (日) (読)
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| 2. | (英) Shima Hiroshi (日) (読)
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| 3. | (英) Watanabe Masahiko (日) (読)
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| 4. | 羽地 達次
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| 5. | (英) Kikuchi Kunimi (日) (読)
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| ○題名 (必須): | □ | (英) Molecular cloning and characterization of a novel dual specificity protein phosphatase possibly involved in spermatogenesis. (日)
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| ○要約 (任意): | □ | (英) Dual-specificity protein phosphatases (DSPs) play roles in the regulation of mitogenic signal transduction for extracellular stimulation and the cell cycle. In the present study, we identified a novel DSP, termed TMDP (testis- and skeletal-muscle-specific DSP). Nucleotide sequence analysis of TMDP cDNA indicated that the open reading frame of 597 bp encodes a protein of 198 amino acid residues with a predicted molecular mass of 22.5 kDa. The deduced amino acid sequence contains a motif for a conserved catalytic domain of DSPs and shows highest similarity to human Vaccinia HI-related phosphatase (45.5% identity) but low homology to the mitogen-activated protein kinase phosphatase and CDC25 subfamilies of DSPs. Recombinant TMDP protein exhibited intrinsic phosphatase activity towards both phospho-seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Northern-blot analysis revealed that TMDP is most abundantly expressed in the testis. The expression in the testis is characterized as follows: (i) TMDP mRNA first appeared 3 weeks after birth, corresponding to the time that meiosis begins; (ii) TMDP mRNA was abundant in fractionated spermatocytes and round spermatids; and (iii) hybridization in situ showed that the TMDP mRNA is localized in spermatocytes and/or spermatids in seminiferous tubules. These data demonstrate that TMDP is a novel DSP abundantly expressed in the testis and suggest that TMDP may be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis. (日)
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| ○キーワード (推奨): | 1. | (英) Amino Acid Sequence (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Base Sequence (日) (読)
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| 4. | (英) Cloning, Molecular (日) (読)
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| 5. | (英) Dual-Specificity Phosphatases (日) (読)
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| 6. | (英) Gene Expression Regulation, Developmental (日) (読)
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| 7. | (英) In Situ Hybridization (日) (読)
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| 8. | (英) Male (日) (読)
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| 9. | (英) Mice (日) (読)
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| 10. | (英) Molecular Sequence Data (日) (読)
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| 11. | (英) Phosphoprotein Phosphatases (日) (読)
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| 12. | (英) Protein Tyrosine Phosphatases (日) (読)
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| 13. | (英) RNA, Messenger (日) (読)
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| 14. | (英) Recombinant Proteins (日) (読)
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| 15. | (英) Spermatocytes (日) (読)
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| 16. | (英) Spermatogenesis (日) (読)
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| 17. | (英) Substrate Specificity (日) (読)
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| 18. | (英) Testis (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)
| ○ISSN (任意): | □ | 0264-6021
ISSN: 0264-6021
(pISSN: 0264-6021, eISSN: 1470-8728) Title: The Biochemical journalTitle(ISO): Biochem JPublisher: Portland Press Ltd (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 344
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 819 825
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1999年 1月 初日 (平成 11年 1月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1042/bj3440819 (→Scopusで検索)
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| ○PMID (任意): | □ | 10585869 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.DataBankList.DataBank.DataBankName: GENBANK (日)
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| 2. | (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB027003 (日)
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| 3. | (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB027004 (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 5. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 6. | (英) OtherID: PMC1220704 (日)
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