『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=70903)

EID=70903EID:70903, Map:0, LastModified:2018年2月6日(火) 14:31:14, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[副研究部長]/[徳島大学.大学院医歯薬学研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1.羽地 達次
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.森本 景之
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Morimoto Yasuhiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Shirakawa Satoko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Kobayashi Shigeru (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Kaneda Chihiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Shima Hiroshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Nagao Minako (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Subcellular localization of protein phosphatase type 1 isotypes in mouse osteoblastic cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The cytolocalization of protein phosphatase type 1 catalytic subunits in exponentially growing mouse osteoblastic MC3T3-E1 cells was determined. Formaldehyde-fixed and alcohol-permeabilized cultured cells were reacted with the PP1 alpha, PP1 delta, PP1 gamma 1, and PP1 gamma 2 antibodies using immunohistochemical methods. With PP1 alpha antibody intense staining occurred in the nuclei, while with PP1 delta antibody nucleolus-like bodies were intensely stained. PP1 gamma 1 localized in the perinuclear region and in the nucleus of the cultured cells, with the staining reaction of the former being much stronger than that in the latter. An immunoreaction did not occur in the cells interacted with PP1 gamma 2 antibody or with the normal rabbit serum. Proteins were prepared from the exponentially growing cells and subconfluent cells. Cellular fractionation was also done with the exponentially growing cells and proteins were prepared from each fraction. Each protein preparation was subjected to SDS-PAGE followed by Western blot analysis with the antibodies. PP1 alpha recognized the 38 kDa proteins mainly present in the nucleus, whereas PP1 delta interacted with the proteins in the nucleolar fraction whose molecular weight was estimated as 37 kDa. PP1 gamma 1 antibody recognized a band corresponding to an estimated molecular weight of 36 kDa mainly in the cytosolic fraction. PP1 gamma 2 antibody and the normal rabbit serum did not interact with any proteins prepared from the cultured cells. Our observations show that four different isozymes of protein phosphatases occupy distinct compartments in MC3T3-E1 cells. This differential distribution suggests that these isozymes may play different roles in cellular functions.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antibodies (日) (読) [継承]
4. (英) Blotting, Western (日) (読) [継承]
5. (英) Cell Line (日) (読) [継承]
6. (英) Cell Nucleolus (日) (読) [継承]
7. (英) Cell Nucleus (日) (読) [継承]
8.免疫組織化学 (immunohistochemistry) [継承]
9. (英) Isoenzymes (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Molecular Sequence Data (日) (読) [継承]
12. (英) Osteoblasts (日) (読) [継承]
13. (英) Phosphoprotein Phosphatases (日) (読) [継承]
14. (英) Subcellular Fractions (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)

ISSN (任意): 0006-291X
ISSN: 0006-291X (pISSN: 0006-291X, eISSN: 1090-2104)
Title: Biochemical and biophysical research communications
Title(ISO): Biochem Biophys Res Commun
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 248 [継承]
(必須): 1 [継承]
(必須): 39 43 [継承]
都市 (任意):
年月日 (必須): 西暦 1998年 1月 初日 (平成 10年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1006/bbrc.1998.8913    (→Scopusで検索) [継承]
PMID (任意): 9675082    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000074758800008 [継承]
Scopus (任意): 2-s2.0-0032499890 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tatsuji Haneji, Hiroyuki Morimoto, Yasuhiro Morimoto, Satoko Shirakawa, Shigeru Kobayashi, Chihiro Kaneda, Hiroshi Shima and Minako Nagao : Subcellular localization of protein phosphatase type 1 isotypes in mouse osteoblastic cells., Biochemical and Biophysical Research Communications, Vol.248, No.1, 39-43, 1998.
欧文冊子 ● Tatsuji Haneji, Hiroyuki Morimoto, Yasuhiro Morimoto, Satoko Shirakawa, Shigeru Kobayashi, Chihiro Kaneda, Hiroshi Shima and Minako Nagao : Subcellular localization of protein phosphatase type 1 isotypes in mouse osteoblastic cells., Biochemical and Biophysical Research Communications, Vol.248, No.1, 39-43, 1998.

関連情報

Number of session users = 2, LA = 0.72, Max(EID) = 383292, Max(EOID) = 1024451.