| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日)
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| ○著者 (必須): | 1. | (英) Murata Takatoshi (日) (読)
| ○役割 (任意): | □ | 共著
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | (英) Ansai Toshihiro (日) (読)
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| 3. | (英) Takehara Tadamichi (日) (読)
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| 4. | (英) Kobayashi Shigeru (日) (読)
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| 5. | 羽地 達次
| ○役割 (任意): | □ | 共著
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| ○貢献度 (任意): |
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| ○題名 (必須): | □ | (英) Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) In order to study the effects of sonicated extracts from Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and other oral-related bacteria, as well as Escherichia coli on bone formation, clone MC3T3-E1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. The addition of the sonicated extracts from Prevotella intermedia and Actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose-dependent fashion over the concentration range of 1-1000 ng ml-1 compared with the control. By contrast, the sonicated extracts from other oral bacteria including Porphyromonas gingivalis, Capnocytophaga ochracea, Streptococcus milleri and Streptococcus sanguis, and Escherichia coli did not decrease the alkaline phosphatase activity even in the presence of 100 ng ml-1 protein. The addition of Prevotella intermedia and Actinobacillus actinomycetemcomitans extracts that had been treated with heat and trypsin to the cell cultures also inhibited alkaline phosphatase activity in the cells, suggesting that inhibitory factors are not proteinaceous. Polymyxin B did not change the alkaline phosphatase activity in the cells treated with the extracts from Prevotella intermedia and Actinobacillus actinomycetemcomitans, suggesting that the inhibitory activity of the extracts is not lipopolysaccharide. The inhibitory effect of both extracts was observed in the molecular mass over 290 kDa eluted from Sephadex G-200 column. The inhibitory substances of Prevotella intermedia were partially purified and showed broad band with estimated molecular weight of 170-190 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results indicate that Prevotella intermedia and Actinobacillus actinomycetemcomitans may play an important role in inhibiting bone formation as well as in stimulating bone resorption. (日)
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| ○キーワード (推奨): | 1. | (英) 3T3 Cells (日) (読)
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| 2. | (英) Aggregatibacter actinomycetemcomitans (日) (読)
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| 3. | (英) Alkaline Phosphatase (日) (読)
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| 4. | (英) Animals (日) (読)
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| 5. | (英) Bacterial Proteins (日) (読)
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| 6. | (英) Clone Cells (日) (読)
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| 7. | (英) Culture Media, Conditioned (日) (読)
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| 8. | (英) Dose-Response Relationship, Drug (日) (読)
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| 9. | (英) Enzyme Inhibitors (日) (読)
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| 10. | (英) Lipopolysaccharides (日) (読)
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| 11. | (英) Mice (日) (読)
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| 12. | 分子量 (molecular weight)
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| 13. | (英) Osteoblasts (日) (読)
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| 14. | (英) Osteogenesis (日) (読)
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| 15. | (英) Prevotella intermedia (日) (読)
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| 16. | (英) Sonication (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Oral Diseases (Munksgaard)
(pISSN: 1354-523X, eISSN: 1601-0825)
| ○ISSN (任意): | □ | 1354-523X
ISSN: 1354-523X
(pISSN: 1354-523X, eISSN: 1601-0825) Title: Oral diseasesTitle(ISO): Oral DisPublisher: Blackwell (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 3
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 106 112
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1997年 1月 初日 (平成 9年 1月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1111/j.1601-0825.1997.tb00021.x (→Scopusで検索)
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| ○PMID (任意): | □ | 9467351 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Comparative Study (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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