| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日)
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| ○著者 (必須): | 1. | 岡村 裕彦
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| ○学籍番号 (推奨): |
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| 2. | 森本 景之
| ○役割 (任意): | □ | 共著
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 3. | 羽地 達次
| ○役割 (任意): | □ | 共著
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| ○題名 (必須): | □ | (英) Peplomycin-induced apoptosis in oral squamous carcinoma cells depend on bleomycin sensitivity. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamous carcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 microM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 microM peplomycin. However, SCCTF cells treated with 1 microM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5 and 50 microM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin. (日)
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| ○キーワード (推奨): | 1. | (英) Antibiotics, Antineoplastic (日) (読)
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| 2. | アポトーシス (apoptosis)
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| 3. | (英) Bleomycin (日) (読)
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| 4. | (英) Carcinoma, Squamous Cell (日) (読)
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| 5. | (英) Colorimetry (日) (読)
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| 6. | (英) DNA Fragmentation (日) (読)
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| 7. | (英) Dose-Response Relationship, Drug (日) (読)
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| 8. | (英) Drug Resistance, Neoplasm (日) (読)
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| 9. | (英) Humans (日) (読)
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| 10. | (英) Microscopy, Phase-Contrast (日) (読)
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| 11. | (英) Mitomycin (日) (読)
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| 12. | (英) Mouth Neoplasms (日) (読)
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| 13. | (英) Peplomycin (日) (読)
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| 14. | (英) Treatment Outcome (日) (読)
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| 15. | (英) Tumor Cells, Cultured (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Oral Oncology (European Association of Oral Medicine/International Association of Oral Pathologists/International Academy of Oral Oncology)
(pISSN: 1368-8375, eISSN: 1879-0593)
| ○ISSN (任意): | □ | 1368-8375
ISSN: 1368-8375
(pISSN: 1368-8375, eISSN: 1879-0593) Title: Oral oncologyTitle(ISO): Oral OncolPublisher: Elsevier Ltd (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 37
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| ○号 (必須): | □ | 4 1
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| ○頁 (必須): | □ | 379 385
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2001年 6月 初日 (平成 13年 6月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/S1368-8375(00)00101-9 (→Scopusで検索)
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| ○PMID (任意): | □ | 11337271 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): | □ | 2-s2.0-0035033075
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| ○評価値 (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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