『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=70750EID:70750, Map:0, LastModified:2022年2月14日(月) 11:55:20, Operator:[三木 ちひろ], Avail:TRUE, Censor:承認済, Owner:[二宮 雅美], Read:継承, Write:継承, Delete:継承.
○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
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○カテゴリ (推奨):
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨):
○著者 (必須): 1.片岡 正俊
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2. (英) SHIMIZU YASUKI (日) (読)
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3. (英) KUNIKIYO KENJI (日) (読)
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4. (英) ASAHARA YOJI (日) (読)
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5.山下 菊治
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6.二宮 雅美 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
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7. (英) Morisaki Ichijiro (日) (読)
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8. (英) Ohsaki Yasuyoshi (日) (読)
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9.木戸 淳一
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10.永田 俊彦
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○題名 (必須): □(英) Cyclosporin A Decreases the Degradation of Type I Collagen in Rat Gingival Overgrowth  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of fibrous gingival overgrowth. The purpose of this study was to investigate the effect of CsA on the type I collagen metabolism in the gingiva of rats fed a powdered diet either containing or lacking CsA. Immunohistochemical analysis revealed that type I collagen was more prevalent in the connective tissue of CsA-treated gingiva than in those of control rats on days 15, 30, and 55 after the start of feeding. Total RNAs were isolated from mandibular molar gingiva on days 0, 3, 8, 15, 30, and 55. Quantitative analysis of mRNA by reverse transcriptase-polymerase chain reaction revealed that the CsA-treated groups showed a gradual decrease in expression of type I collagen and collagenase mRNAs, 0.4% and 18.0% on day 55 compared with those on day 0, respectively. In the control groups, type I collagen and collagenase mRNAs also decreased to 19.7% and 63.0%, respectively, however, both mRNA expressions were significantly lower in the CsA-treated group than in the controls. An electron microscopic analysis of fibroblasts was performed to count the number of cells with collagen fibrils in the cytoplasm, a marker of phagocytosis of collagen by fibroblasts. The collagen fibrils were detected in 4.7% +/- 2.7% and 24.3% +/- 13.7% of fibroblasts in the overgrown gingiva treated with CsA rat for 8 days and 30 days, but in 57.0% +/- 5.3% and 81.3% +/- 9.2% of fibroblasts in the each control group gingiva, respectively. Furthermore, in vitro analysis was performed to measure the phagocytosis of cultured fibroblasts by flow cytometry using collagen-coated latex beads. Fibroblasts isolated from CsA-treated gingiva on day 8 and day 30 contained 5.7% +/- 0.6% and 9.9% +/- 1.5% phagocytic cells, whereas control fibroblasts contained 50.3% +/- 5.5% and 33.3% +/- 4.9% phagocytic cells, respectively. The inhibition rate of phagocytic activity was similar between in vivo and in vitro assays. These findings suggest that the decrease of the collagen degradation due to the lower phagocytosis and the lower collagenase mRNA expression are closely associated with the increase of type I collagen accumulation in CsA-treated rat gingiva.  (日)    [継承]
○キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cell Division (日) (読) [継承]
3. (英) Cells, Cultured (日) (読) [継承]
4. (英) Collagen (日) (読) [継承]
5. (英) Collagenases (日) (読) [継承]
6. (英) Cyclosporine (日) (読) [継承]
7. (英) Fibroblasts (日) (読) [継承]
8. (英) Gene Expression (日) (読) [継承]
9. (英) Gingiva (日) (読) [継承]
10. (英) Gingival Overgrowth (日) (読) [継承]
11. (英) Immunosuppressive Agents (日) (読) [継承]
12. (英) Latex (日) (読) [継承]
13.男性 (male) [継承]
14. (英) Microscopy, Electron (日) (読) [継承]
15. (英) Microspheres (日) (読) [継承]
16. (英) Phagocytosis (日) (読) [継承]
17. (英) RNA, Messenger (日) (読) [継承]
18. (英) Rats (日) (読) [継承]
19. (英) Rats, Inbred F344 (日) (読) [継承]
20. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
21. (英) Specific Pathogen-Free Organisms (日) (読) [継承]
○発行所 (推奨): □Wiley-Liss, Inc. [継承]
○誌名 (必須): □Journal of Cellular Physiology ([Wiley-Liss, Inc.])
(pISSN: 0021-9541, eISSN: 1097-4652)

○ISSN (任意): □0021-9541
ISSN: 0021-9541 (pISSN: 0021-9541, eISSN: 1097-4652)
Title: Journal of cellular physiology
Title(ISO): J Cell Physiol
Publisher: Wiley
 (NLM Catalog)   (Wiley)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □182 [継承]
○号 (必須): □3 [継承]
○頁 (必須): □351 358 [継承]
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○年月日 (必須): □西暦 2000年 9月 20日 (平成 12年 9月 20日) [継承]
○URL (任意): □http://www3.interscience.wiley.com/cgi-bin/abstract/69501962/ABSTRACT [継承]
○DOI (任意): □10.1002/(SICI)1097-4652(200003)182:3<351::AID-JCP5>3.0.CO;2-U    (→Scopusで検索) [継承]
○PMID (任意): □10653601    (→Scopusで検索) [継承]
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○Scopus (任意): □2-s2.0-0033957168 [継承]
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○備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Masatoshi Kataoka, YASUKI SHIMIZU, KENJI KUNIKIYO, YOJI ASAHARA, Kikuji Yamashita, Masami Ninomiya, Ichijiro Morisaki, Yasuyoshi Ohsaki, Jun-ichi Kido and Toshihiko Nagata : Cyclosporin A Decreases the Degradation of Type I Collagen in Rat Gingival Overgrowth, Journal of Cellular Physiology, Vol.182, No.3, 351-358, 2000.
欧文冊子 ● Masatoshi Kataoka, YASUKI SHIMIZU, KENJI KUNIKIYO, YOJI ASAHARA, Kikuji Yamashita, Masami Ninomiya, Ichijiro Morisaki, Yasuyoshi Ohsaki, Jun-ichi Kido and Toshihiko Nagata : Cyclosporin A Decreases the Degradation of Type I Collagen in Rat Gingival Overgrowth, Journal of Cellular Physiology, Vol.182, No.3, 351-358, 2000.

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