『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=70743EID:70743, Map:0, LastModified:2018年2月8日(木) 14:02:54, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1. (英) Kito Shinji (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Shimizu Katsuhide (日) (読)
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
3.岡村 裕彦
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
4.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Fujita Michi (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
6.森本 景之
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
7. (英) Morimoto Yasuhiro (日) (読)
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
8. (英) Ohba Takeshi (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
9.羽地 達次
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Cleavage of nucleolin and argyrophilic nucleolar organizer region associated proteins in  (日)    [継承]
副題 (任意):
要約 (任意): (英) To investigate the behavior of nuclear proteins in apoptotic cells, we examined the changes in nucleolin and proteins of the nucleolar organizing region during apoptosis in human osteoblastic cell lines, Saos-2 and MG63. Apoptosis was induced by treatment of these cells with okadaic acid. Proteins prepared from apoptotic cells were subjected to Western blot analysis and a modified Western blot method using silver nitrate. The anti-nucleolin antibody recognized the 110-kDa band and the staining intensity of this band decreased in the proteins prepared from the okadaic acid-treated apoptotic cells. The additional band of an 80-kDa was also detected in the proteins prepared from the apoptotic cells. Two major silver nitrate-stained bands, 110-kDa and 37-kDa, were detected among the proteins obtained from control cells. Like the Western blot analysis, the intensity of the 110-kDa silver nitrate-staining band decreased; an 80-kDa band appeared and its staining intensity increased in the lysate from the okadaic acid-treated cells. The signal intensity of the 37-kDa protein did not change in the sample from the apoptotic cells. In a cell-free apoptotic system, the 80-kDa protein was also detected and the amount of the 110-kDa protein decreased in the extract of Saos-2 cell nuclei incubated with apoptotic cytosol. The change in nucleolin in Saos-2 cells induced to undergo apoptosis was examined by an immunocytochemical procedure using the anti-nucleolin antibody and Hoechst 33342. Nucleolin was visible as dots in nucleoli in the control cells; however, it was not detected in the cells undergoing apoptosis. The dual-exposure view of Hoechst 33342 and anti-nucleolin staining cells confirmed that nucleolin had disappeared from the apoptotic nuclei of Saos-2.  (日)    [継承]
キーワード (推奨): 1. (英) Apoptosis (日) (読) [継承]
2. (英) Blotting, Western (日) (読) [継承]
3. (英) Cell Fractionation (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Cell Nucleolus (日) (読) [継承]
6. (英) Cell Nucleus (日) (読) [継承]
7. (英) Cell-Free System (日) (読) [継承]
8. (英) DNA Fragmentation (日) (読) [継承]
9. (英) Enzyme Inhibitors (日) (読) [継承]
10. (英) Fluorescent Dyes (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Immunohistochemistry (日) (読) [継承]
13. (英) Nuclear Proteins (日) (読) [継承]
14. (英) Nucleolus Organizer Region (日) (読) [継承]
15. (英) Okadaic Acid (日) (読) [継承]
16. (英) Osteoblasts (日) (読) [継承]
17. (英) Phosphoproteins (日) (読) [継承]
18. (英) RNA-Binding Proteins (日) (読) [継承]
19. (英) Silver Nitrate (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)

ISSN (任意): 0006-291X
ISSN: 0006-291X (pISSN: 0006-291X, eISSN: 1090-2104)
Title: Biochemical and biophysical research communications
Title(ISO): Biochem Biophys Res Commun
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 300 [継承]
(必須): 4 [継承]
(必須): 950 956 [継承]
都市 (任意):
年月日 (必須): 西暦 2003年 1月 24日 (平成 15年 1月 24日) [継承]
URL (任意):
DOI (任意): 10.1016/S0006-291X(02)02942-X    (→Scopusで検索) [継承]
PMID (任意): 12559966    (→Scopusで検索) [継承]
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Scopus (任意):
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, Kyushu Dental College, Kitakyushu, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Shinji Kito, Katsuhide Shimizu, Hirohiko Okamura, Kaya Yoshida, Michi Fujita, Hiroyuki Morimoto, Yasuhiro Morimoto, Takeshi Ohba and Tatsuji Haneji : Cleavage of nucleolin and argyrophilic nucleolar organizer region associated proteins in, Biochemical and Biophysical Research Communications, Vol.300, No.4, 950-956, 2003.
欧文冊子 ● Shinji Kito, Katsuhide Shimizu, Hirohiko Okamura, Kaya Yoshida, Michi Fujita, Hiroyuki Morimoto, Yasuhiro Morimoto, Takeshi Ohba and Tatsuji Haneji : Cleavage of nucleolin and argyrophilic nucleolar organizer region associated proteins in, Biochemical and Biophysical Research Communications, Vol.300, No.4, 950-956, 2003.

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