『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.医学部.保健学科 [継承]
著者 (必須): 1.細井 英司 ([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.医用検査学系.細胞・免疫解析学]/[徳島大学.医学部.保健学科.検査技術科学専攻.機能系検査学講座])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Nishizaki Chiharu (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Gallagher L. Kathleen (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Wyre W. Hadley (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Matsuo Yoshinobu (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Sei Yoshitatsu (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Expression of the ryanodine receptor isoforms in immune cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Ryanodine receptor (RYR) is a Ca(2+) channel that mediates Ca(2+) release from intracellular stores. We have used RT-PCR analysis and examined its expression in primary peripheral mononuclear cells (PBMCs) and in 164 hemopoietic cell lines. In PBMCs, type 1 RYR (RYR1) was expressed in CD19(+) B lymphocytes, but less frequently in CD3(+) T lymphocytes and in CD14(+) monocytes. Type 2 RYR (RYR2) was mainly detected in CD3(+) T cells. Induction of RYR1 and/or RYR2 mRNA was found after treatment with stromal cell-derived factor 1, macrophage-inflammatory protein-1alpha (MIP1alpha) or TGF-beta. Type 3 RYR (RYR3) was not detected in PBMCs. Many hemopoietic cell lines expressed not only RYR1 or RYR2 but also RYR3. The expression of the isoforms was not associated with specific cell lineage. We showed that the RYR-stimulating agent 4-chloro-m-cresol (4CmC) induced Ca(2+) release and thereby confirmed functional expression of the RYR in the cell lines expressing RYR mRNA. Moreover, concordant induction of RYR mRNA with Ca(2+) channel function was found in Jurkat T cells. In untreated Jurkat T cells, 4CmC (>1 mM) had no effect on Ca(2+) release, whereas 4CmC (<400 microM) caused Ca(2+) release after the induction of RYR2 and RYR3 that occurred after treatment with stromal cell-derived factor 1, macrophage-inflammatory protein-1alpha, or TGF-beta. Our results demonstrate expression of all three isoforms of RYR mRNA in hemopoietic cells. Induction of RYRs in response to chemokines and TGF-beta suggests roles in regulating Ca(2+)-mediated cellular responses during the immune response.  (日)    [継承]
キーワード (推奨): 1. (英) Calcium (日) (読) [継承]
2. (英) Cell Line (日) (読) [継承]
3. (英) Hematopoietic System (日) (読) [継承]
4. (英) Humans (日) (読) [継承]
5. (英) Leukocytes, Mononuclear (日) (読) [継承]
6. (英) Protein Isoforms (日) (読) [継承]
7. (英) RNA, Messenger (日) (読) [継承]
8. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
9. (英) Ryanodine Receptor Calcium Release Channel (日) (読) [継承]
10. (英) Transforming Growth Factor beta (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
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(必須): 167 [継承]
(必須): 9 [継承]
(必須): 4887 4894 [継承]
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年月日 (必須): 西暦 2001年 9月 初日 (平成 13年 9月 初日) [継承]
URL (任意):
DOI (任意): 10.4049/jimmunol.167.9.4887    (→Scopusで検索) [継承]
PMID (任意): 11673493    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000171858500014 [継承]
Scopus (任意): 2-s2.0-0035500806 [継承]
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指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]

標準的な表示

和文冊子 ● Eiji Hosoi, Chiharu Nishizaki, Kathleen L. Gallagher, Hadley W. Wyre, Yoshinobu Matsuo and Yoshitatsu Sei : Expression of the ryanodine receptor isoforms in immune cells, The Journal of Immunology, Vol.167, No.9, 4887-4894, 2001.
欧文冊子 ● Eiji Hosoi, Chiharu Nishizaki, Kathleen L. Gallagher, Hadley W. Wyre, Yoshinobu Matsuo and Yoshitatsu Sei : Expression of the ryanodine receptor isoforms in immune cells, The Journal of Immunology, Vol.167, No.9, 4887-4894, 2001.

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