『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.分子酵素学研究センター (〜2007年3月31日/->組織[徳島大学.疾患酵素学研究センター]) [継承]
2.徳島大学.医学部.医学科.情報統合医学講座 [継承]
著者 (必須): 1. (英) Yuasa Chie (日) 湯浅 智恵 (読) ゆあさ ちえ
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2.冨田 優美子
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3. (英) Shono Masayuki (日) 庄野 正行 (読) しょうの まさゆき
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4.石村 和敬
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5. (英) Ichihara Akira (日) 市原 明 (読) いちはら あきら
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題名 (必須): (英) Importance of cell aggregation for expression of liver functions and regeneration demonstrated with primary cultured hepatocytes  (日)    [継承]
副題 (任意):
要約 (任意): (英) Adult rat hepatocytes aggregated to form floating multicellular spheroids when cultured in Primaria dishes, which have a positively charged surface, in serum-free Williams' medium E (WE) supplemented with insulin and epidermal growth factor (EGF). These hormones were essential for maintenance of the spheroids, whereas the size of the spheroids depended on the inoculum cell density. The spheroids retained in vivo levels of expressions of albumin and glucokinase and synthesized scarcely any DNA even in the presence of insulin and EGF. On transfer to type I collagen-coated dishes, the spheroids gradually disaggregated and the cells formed monolayers, in which the expressions of albumin and glucokinase were suppressed and DNA synthesis and hexokinase activity were increased. DNA synthesis of hepatocytes in monolayer culture was maximal 24 hr after transfer of the spheroids, approximately 80% of the hepatocyte nuclei were labelled with bromodeoxyuridine during culture for 48 hr, and the mitotic index was approximately 70% after 60 hr. These results suggest that, in spheroids, hepatocytes remained in the G0 phase, but that when they formed monolayers, they progressed to the G1 phase and proceeded through the cell cycle in the presence of insulin and EGF. This work shows that the cell cycle of hepatocytes in culture can be manipulated by providing conditions for quiescence as spheroids or growth as monolayers and that the shape of hepatocytes is important for regulating their growth and liver-specific functions.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cell Aggregation (日) (読) [継承]
3. (英) Cells, Cultured (日) (読) [継承]
4.DNA (DNA) [継承]
5. (英) Deoxyuridine (日) (読) [継承]
6. (英) Epidermal Growth Factor (日) (読) [継承]
7. (英) Glucokinase (日) (読) [継承]
8. (英) Hexokinase (日) (読) [継承]
9.インスリン (insulin) [継承]
10. (英) Liver (日) (読) [継承]
11. (英) Liver Regeneration (日) (読) [継承]
12. (英) Microscopy, Electron (日) (読) [継承]
発行所 (推奨): Wiley-Liss, Inc. [継承]
誌名 (必須): Journal of Cellular Physiology ([Wiley-Liss, Inc.])
(pISSN: 0021-9541, eISSN: 1097-4652)

ISSN (任意): 0021-9541
ISSN: 0021-9541 (pISSN: 0021-9541, eISSN: 1097-4652)
Title: Journal of cellular physiology
Title(ISO): J Cell Physiol
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 156 [継承]
(必須): 3 [継承]
(必須): 522 530 [継承]
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年月日 (必須): 西暦 1993年 1月 初日 (平成 5年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1002/jcp.1041560311    (→Scopusで検索) [継承]
PMID (任意): 8360258    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Chie Yuasa, Yumiko Tomita, Masayuki Shono, Kazunori Ishimura and Akira Ichihara : Importance of cell aggregation for expression of liver functions and regeneration demonstrated with primary cultured hepatocytes, Journal of Cellular Physiology, Vol.156, No.3, 522-530, 1993.
欧文冊子 ● Chie Yuasa, Yumiko Tomita, Masayuki Shono, Kazunori Ishimura and Akira Ichihara : Importance of cell aggregation for expression of liver functions and regeneration demonstrated with primary cultured hepatocytes, Journal of Cellular Physiology, Vol.156, No.3, 522-530, 1993.

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