『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=68733EID:68733, Map:0, LastModified:2013年6月17日(月) 16:20:02, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[センター長]/[徳島大学.疾患酵素学研究センター]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨): 1.徳島大学.分子酵素学研究センター (〜2007年3月31日/->組織[徳島大学.疾患酵素学研究センター]) [継承]
著者 (必須): 1. (英) Ishii Kazuo (日) (読)
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学籍番号 (推奨):
[継承]
2. (英) Hayashi Hideki (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Todaka Mikio (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Kamohara Seika (日) (読)
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[継承]
5. (英) Kanai Fumihiko (日) (読)
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[継承]
6. (英) Jinnouchi Hideaki (日) (読)
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[継承]
7. (英) Wang Lihong (日) (読)
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[継承]
8.蛯名 洋介
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[継承]
題名 (必須): (英) Possible domains responsible for intracellular targeting and insulin-dependent translocation of glucose transporter type 4.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Translocation of the type 4 glucose transporter (GLUT4) to the cell surface from an intracellular pool is the major mechanism of insulin-stimulated glucose uptake in insulin-target cells. We developed a highly sensitive and quantitative method to detect GLUT4 immunologically on the surface of intact cells, using c-myc epitope-tagged GLUT4 (GLUT4myc). We constructed c-myc epitope-tagged glucose transporter type 1 (GLUT1myc) and found that the GLUT1myc was also translocated to the cell surface of Chinese hamster ovary cells, 3T3-L1 fibroblasts and NIH 3T3 cells, in response to insulin, but the degree of translocation was less than that of GLUT4myc. Since GLUT1 and GLUT4 have different intracellular distributions and different degrees of insulin-stimulated translocation, we examined the domains of GLUT4, using c-myc epitope-tagged chimeric glucose transporters between these two isoforms. The results indicated that, (1) all the cytoplasmic N-terminal region, middle intracellular loop and cytoplasmic C-terminal region of GLUT4 have independent intracellular targeting signals, (2) these sequences for intracellular targeting of GLUT4 were not sufficient to determine GLUT4 translocation in response to insulin, and (3) the N-terminal half of GLUT4 devoid both of cytoplasmic N-terminus and of middle intracellular loop seems to be necessary for insulin-stimulated GLUT4 translocation.  (日)    [継承]
キーワード (推奨): 1. (英) 3T3 Cells (日) (読) [継承]
2. (英) Amino Acid Sequence (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Biological Transport (日) (読) [継承]
5. (英) CHO Cells (日) (読) [継承]
6. (英) Cloning, Molecular (日) (読) [継承]
7. (英) Cricetinae (日) (読) [継承]
8. (英) Cytoplasm (日) (読) [継承]
9. (英) Epitopes (日) (読) [継承]
10. (英) Glucose Transporter Type 1 (日) (読) [継承]
11. (英) Glucose Transporter Type 4 (日) (読) [継承]
12.インスリン (insulin) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Molecular Sequence Data (日) (読) [継承]
15. (英) Monosaccharide Transport Proteins (日) (読) [継承]
16. (英) Muscle Proteins (日) (読) [継承]
17. (英) Proto-Oncogene Proteins c-myc (日) (読) [継承]
18. (英) Rats (日) (読) [継承]
19. (英) Recombinant Fusion Proteins (日) (読) [継承]
20. (英) Sodium Fluoride (日) (読) [継承]
21. (英) Subcellular Fractions (日) (読) [継承]
22. (英) Tetradecanoylphorbol Acetate (日) (読) [継承]
発行所 (推奨): The Biochemical Society [継承]
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 309 [継承]
(必須): Pt 3 [継承]
(必須): 813 823 [継承]
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年月日 (必須): 西暦 1995年 8月 1日 (平成 7年 8月 1日) [継承]
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PMID (任意): 7543750    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Enzyme Genetics, University of Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC1135705  (日)    [継承]

標準的な表示

和文冊子 ● Kazuo Ishii, Hideki Hayashi, Mikio Todaka, Seika Kamohara, Fumihiko Kanai, Hideaki Jinnouchi, Lihong Wang and Yousuke Ebina : Possible domains responsible for intracellular targeting and insulin-dependent translocation of glucose transporter type 4., The Biochemical Journal, Vol.309, No.Pt 3, 813-823, 1995.
欧文冊子 ● Kazuo Ishii, Hideki Hayashi, Mikio Todaka, Seika Kamohara, Fumihiko Kanai, Hideaki Jinnouchi, Lihong Wang and Yousuke Ebina : Possible domains responsible for intracellular targeting and insulin-dependent translocation of glucose transporter type 4., The Biochemical Journal, Vol.309, No.Pt 3, 813-823, 1995.

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