『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=67647EID:67647, Map:0, LastModified:2014年4月17日(木) 15:45:20, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[福井 清], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨): 博士課程学生による研究報告 [継承]
組織 (推奨): 1.分子酵素学研究センター (〜2007年3月31日/->組織[疾患酵素学研究センター]) [継承]
著者 (必須): 1. (英) Shimizu Yoshihisa (日) 清水 善久 (読) しみず よしひさ
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2. (英) Akashi Tomohiro (日) (読)
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3. (英) Okuda Asuko (日) (読)
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4. (英) Kikuchi Akihiko (日) (読)
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5.福井 清 ([徳島大学])
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題名 (必須): (英) NBP1 (Nap1 Binding Protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization  (日)    [継承]
副題 (任意):
要約 (任意): (英) Nap1p is identified in mammalian cell extract by its intrinsic activity to facilitate nucleosome assembly in vitro in the physiological ionic condition. The homologous proteins are present in most eukaryotes, and their functional analyses in vitro have suggested that they are necessary to keep proper nucleosome structures in transcription and replication. This protein is also identified for its interaction with Clb2p in vitro. To address the function of Nap1p in vivo, we have surveyed for proteins to interact with Nap1p by two-hybrid system and obtained two genes, NBP1 and NBP2 (Nap1 Binding Protein 1 and 2). NBP1 is an essential gene and encodes a novel protein consisting of 319 amino acids, with a coiled-coil structure in the center of the predicted amino acid sequence. Several A-kinase dependent phosphorylation sites and Cdc28p kinase-dependent sites are also observed. By isolating the temperature-sensitive mutant, we demonstrate that the nuclear division at a non-permissive temperature is delayed and that the population of cells with a large bud carrying a single nucleus with a short spindle are increased. This mutant also confers resistance against benomyl, a microtubule-destabilizing agent. Judging from the green fluorescent protein (GFP) signal fused with Nbp1p, this protein localizes in the nucleus as one or two tiny dots.  (日)    [継承]
キーワード (推奨): 1.細胞周期 (cell cycle) [継承]
2.コイルドコイルモチーフ (coiled-coil motif) [継承]
3.分裂装置 (mitotic apparatus) [継承]
発行所 (推奨): Elsevier Science B.V. [継承]
誌名 (必須): Gene ([Elsevier Science B.V.])
(pISSN: 0378-1119, eISSN: 1879-0038)

ISSN (任意): 0378-1119
ISSN: 0378-1119 (pISSN: 0378-1119, eISSN: 1879-0038)
Title: Gene
Title(ISO): Gene
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 246 [継承]
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年月日 (必須): 西暦 2000年 4月 4日 (平成 12年 4月 4日) [継承]
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DOI (任意): 10.1016/S0378-1119(00)00067-6    (→Scopusで検索) [継承]
PMID (任意): 10767562    (→Scopusで検索) [継承]
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WOS (任意): 000086490600041 [継承]
Scopus (任意): 2-s2.0-0034603853 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yoshihisa Shimizu, Tomohiro Akashi, Asuko Okuda, Akihiko Kikuchi and Kiyoshi Fukui : NBP1 (Nap1 Binding Protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization, Gene, Vol.246, No.1-2, 395-404, 2000.
欧文冊子 ● Yoshihisa Shimizu, Tomohiro Akashi, Asuko Okuda, Akihiko Kikuchi and Kiyoshi Fukui : NBP1 (Nap1 Binding Protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization, Gene, Vol.246, No.1-2, 395-404, 2000.

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