『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.青山学院大学.理工学部 [継承]
著者 (必須): 1. (英) Mizutani Yukihito (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Narikawa Tatsuya (日) (読)
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
3.佐藤 高則
役割 (任意): 共著 [継承]
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学籍番号 (推奨):
[継承]
4. (英) Sakurai Nobuhiko (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Kaji Hiroyuki (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Yamada Shingo (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Samejima Tatsuya (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) A New UV Method for Serum γ-Glutamyltransferase Assay Using Recombinant 4-Aminobenzoate Hydroxylase as a Coupling Enzyme.  (日)    [継承]
副題 (任意):
要約 (任意): (英) 4-aminobenzoate hydroxylase (4ABH) is a flavin-dependent monooxygenase that catalyzes the decarboxylative hydroxylation of 4-aminobenzoate to 4-hydroxyaniline. For use as a clinical reagent, the gene encoding 4ABH from Agaricus bisporus was cloned by the RACE method. Also, the cDNA encoding 4ABH was expressed in Escherichia coli cells as a fusion protein with glutathione S-transferase (GST). The expressed GST-4ABH fusion protein (recombinant 4ABH) in the soluble fraction exhibits decarboxylative hydroxylation and additional NADH oxidation activities.We investigated a new ultraviolet spectrometric method for determining serum gamma-glutamyltransferase (gamma-GT) using recombinant 4ABH as a coupling enzyme. The principle of the method is as follows. Using gamma-glutamyl-3-choloro-4-aminobenzoate (L-gamma-glu-PAClBA) and glycylglycine as the donor and acceptor substrates, 3-choloro-4-aminobenzoate (PAClBA) is formed by the catalysis of serum gamma-GT. PAClBA is stoichiometrically converted to 3-choloro-4-hydroxyaniline (PHClA) and NAD(+) by 4ABH and NADH. However, NADH oxidation results in a high reagent blank, which is considered as a drawback for use as a clinical reagent. Using recombinant 4ABH, we examined the effects of pH and detergents on these two activities, and found that several detergents suppress the additional NADH oxidation activity with little or no effect on hydroxylation activity. The results indicate a promising approach to establishing an ultraviolet spectrophotometric method for determining serum gamma-GT activity using L-gamma-glu-PAClBA as the donor substrate and recombinant 4ABH as a coupling enzyme.  (日) 4-Aminobenzoate Hydroxylase (4-ABH)遺伝子をマッシュルームよりcDNAクローニングを行い,GSTとの融合タンパク質として大腸菌内で効率良く発現させた.この組換え体4-ABHを共役酵素として,血清中のγ-Glutamyltransferaseの測定系について検討を行った.その結果,4-ABHを用いた場合,従来法と良い相関を示し,今後臨床検査への応用が可能となった.   [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Detergents (日) (読) [継承]
3. (英) Dose-Response Relationship, Drug (日) (読) [継承]
4. (英) Escherichia coli (日) (読) [継承]
5. (英) Hydrogen-Ion Concentration (日) (読) [継承]
6. (英) Hydroxylation (日) (読) [継承]
7. (英) Mixed Function Oxygenases (日) (読) [継承]
8. (英) Models, Chemical (日) (読) [継承]
9. (英) Molecular Sequence Data (日) (読) [継承]
10. (英) Multienzyme Complexes (日) (読) [継承]
11. (英) NAD (日) (読) [継承]
12. (英) NADH, NADPH Oxidoreductases (日) (読) [継承]
13. (英) Peroxidases (日) (読) [継承]
14. (英) Protein Engineering (日) (読) [継承]
15. (英) Recombinant Proteins (日) (読) [継承]
16. (英) Sequence Homology, Amino Acid (日) (読) [継承]
17. (英) Spectrophotometry (日) (読) [継承]
18. (英) Ultraviolet Rays (日) (読) [継承]
19. (英) gamma-Glutamyltransferase (日) (読) [継承]
発行所 (推奨): 日本生化学会 [継承]
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 0021-924X
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 126 [継承]
(必須): 2 [継承]
(必須): 347 353 [継承]
都市 (任意):
年月日 (必須): 西暦 1999年 6月 1日 (平成 11年 6月 1日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 10423528    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000082011700014 [継承]
Scopus (任意):
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yukihito Mizutani, Tatsuya Narikawa, Takanori Satoh, Nobuhiko Sakurai, Hiroyuki Kaji, Shingo Yamada and Tatsuya Samejima : A New UV Method for Serum γ-Glutamyltransferase Assay Using Recombinant 4-Aminobenzoate Hydroxylase as a Coupling Enzyme., The Journal of Biochemistry, Vol.126, No.2, 347-353, 1999.
欧文冊子 ● Yukihito Mizutani, Tatsuya Narikawa, Takanori Satoh, Nobuhiko Sakurai, Hiroyuki Kaji, Shingo Yamada and Tatsuya Samejima : A New UV Method for Serum γ-Glutamyltransferase Assay Using Recombinant 4-Aminobenzoate Hydroxylase as a Coupling Enzyme., The Journal of Biochemistry, Vol.126, No.2, 347-353, 1999.

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