『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.青山学院大学.理工学部 [継承]
2.山梨大学 [継承]
3.森永乳業株式会社 [継承]
著者 (必須): 1. (英) Motizuki Mitsuyoshi (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Itoh Takehito (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
3.佐藤 高則
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Yokota Sadaki (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Samejima Tatsuya (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Tsurugi Kunio (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Lipid-binding and antimicrobial properties of synthetic peptides of bovine apolipoprotein A-II.  (日)    [継承]
副題 (任意):
要約 (任意): (英) We previously showed that bovine apolipoprotein A-II (apoA-II) had antimicrobial activity against Escherichia coli and the yeast Saccharomyces cerevisiae in PBS. We have characterized here the active domain of apoA-II using synthetic peptides. A peptide corresponding to C-terminal residues Leu(49)-Thr(76) exhibited significant antimicrobial activity against E. coli in PBS, but not against S. cerevisiae. Experiments using amino-acid-substituted peptides indicated that the residues Phe(52)-Phe(53)-Lys(54)-Lys(55) are required for the activity. Peptide Leu(49)-Thr(76) induced the release of calcein trapped inside the vesicles whose lipid composition resembles that of E. coli membrane, suggesting that peptide Leu(49)-Thr(76) can destabilize the E. coli membrane. CD measurements showed that the alpha-helicity of peptide Leu(49)-Thr(76) increased from 3.5 to 36% by addition of the vesicles. When E. coli cells were incubated with peptide Leu(49)-Thr(76), some proteins were released to the external medium, probably owing to membrane destabilization caused by the peptide. In electron micrographs of E. coli cells treated with peptide Leu(49)-Thr(76), transparent nucleoids and granulated cytoplasm were observed. Amino acid substitutions, Phe(52)Phe(53)-->AlaAla (Phe(52, 53)-->Ala) in peptide Leu(49)-Thr(76) caused the loss of antimicrobial activity against E. coli, although protein-releasing activity was retained. Electron micrographs of the cells treated with peptide Leu(49)-Thr(76)(Phe(52,53)-->Ala) revealed morphological change only at the nucleoids. Therefore peptide Leu(49)-Thr(76) appears to primarily target the cytoplasm rather than the membrane of E. coli cells.  (日) ウシ由来Apolipoprotein A-IIは大腸菌などに対して抗菌作用を示す.本研究では,A-IIの抗菌性に関与する領域を探索するために,種々の欠損体を調製し検討を行った.その結果,Leu49- Thr73peptideは大腸菌に対し抗菌性を示した.さらに,Phe52,53をAlaに置換した変異体や Lys54,55をAlaに置換した変異体では抗菌性の減少が観察され,これらの残基の関与が示唆された.   [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Amino Acid Substitution (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Anti-Bacterial Agents (日) (読) [継承]
5. (英) Anti-Infective Agents (日) (読) [継承]
6. (英) Apolipoprotein A-II (日) (読) [継承]
7. (英) Bacterial Proteins (日) (読) [継承]
8. (英) Cattle (日) (読) [継承]
9. (英) Cell Membrane (日) (読) [継承]
10. (英) Circular Dichroism (日) (読) [継承]
11. (英) Cytoplasm (日) (読) [継承]
12. (英) Escherichia coli (日) (読) [継承]
13. (英) Fluoresceins (日) (読) [継承]
14. (英) Inhibitory Concentration 50 (日) (読) [継承]
15. (英) Microscopy, Electron (日) (読) [継承]
16. (英) Molecular Sequence Data (日) (読) [継承]
17. (英) Molecular Weight (日) (読) [継承]
18. (英) Peptide Fragments (日) (読) [継承]
19. (英) Phospholipids (日) (読) [継承]
20. (英) Protein Binding (日) (読) [継承]
21. (英) Protein Structure, Secondary (日) (読) [継承]
22. (英) Saccharomyces cerevisiae (日) (読) [継承]
23. (英) Sodium Chloride (日) (読) [継承]
発行所 (推奨): Portland Press [継承]
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 342 [継承]
(必須): 1 [継承]
(必須): 215 221 [継承]
都市 (任意): ロンドン (London/[連合王国]) [継承]
年月日 (必須): 西暦 1999年 6月 17日 (平成 11年 6月 17日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 10432319    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000082261200028 [継承]
Scopus (任意):
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指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Mitsuyoshi Motizuki, Takehito Itoh, Takanori Satoh, Sadaki Yokota, Tatsuya Samejima and Kunio Tsurugi : Lipid-binding and antimicrobial properties of synthetic peptides of bovine apolipoprotein A-II., The Biochemical Journal, Vol.342, No.1, 215-221, 1999.
欧文冊子 ● Mitsuyoshi Motizuki, Takehito Itoh, Takanori Satoh, Sadaki Yokota, Tatsuya Samejima and Kunio Tsurugi : Lipid-binding and antimicrobial properties of synthetic peptides of bovine apolipoprotein A-II., The Biochemical Journal, Vol.342, No.1, 215-221, 1999.

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