『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.青山学院大学.理工学部 [継承]
2.金沢大学 [継承]
3.天野製薬株式会社 [継承]
著者 (必須): 1. (英) Shimizu Atsushi (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Kwon Jung-Hee (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Sasaki Takashi (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.佐藤 高則
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Sakurai Nobuhiko (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Sakurai Takeshi (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Yamaguchi Shotaro (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Samejima Tatsuya (日) (読)
役割 (任意): 共著 [継承]
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Myrothecium verrucaria Bilirubin Oxidase and Its Mutants for Potential Copper-Ligands.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Bilirubin oxidase (EC:1.3.3.5) purified from a culture medium of Myrothecium verrucaria MT-1 (authentic enzyme) catalyzes the oxidation of bilirubin to biliverdin in vitro and recombinant enzyme (wild type) was obtained by using an overexpression system of the bilirubin oxidase gene with Aspergillus oryzae harboring an expression vector. The absorption and ESR spectra showed that both bilirubin oxidases are multicopper oxidases containing type 1, type 2, and type 3 coppers similar to laccase, ascorbate oxidase, and ceruloplasmin. Site-directed mutagenesis has been performed for the possible ligands of each type of copper. In some mutants, Cys457 --> Val, Ala, His94 --> Val, and His134.136 --> Val, type 1 and type 2 copper centers were perturbed completely and the enzyme activity was completely lost. Differing from the holoenzyme, these mutants showed type 3 copper signals. However, the optical and magnetic properties characteristic of type 1 copper were retained even by mutating one of the type 1 copper ligands, i.e., a mutant, Met467 --> Gly, showed a weak but apparent enzyme activity. A double mutant His456.458 --> Val had only type 1 Cu, showing a blue band at 600 nm (epsilon = 1.6 x 10(3)) and an ESR signal with very narrow hyperfine splitting (A parallel = 7.2 x 10(-)3 cm-1). Since the type 2 and type 3 coppers are not present, the mutant did not show enzyme activity. These results strongly imply that the peculiar sequence in bilirubin oxidase, His456-Cys457-His458, forms an intramolecular electron-transfer pathway between the type 1 copper site and the trinuclear center composed of the type 2 and type 3 copper sites.  (日) Myrothecium verrucaria 由来Bilirubin Oxidase遺伝子を麹菌を用いた発現系により発現させた.また,部位特異的変異法により,本酵素の銅原子リガンドと推定される部位に変異を導入した変異体を,ESRや吸収スペクトルにより分析し,銅原子リガンドを推定した.その結果より,本酵素の銅原子個数およびそのタイプや電子伝達経路についても考察を行った.   [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Aspergillus oryzae (日) (読) [継承]
3. (英) Copper (日) (読) [継承]
4. (英) Electron Spin Resonance Spectroscopy (日) (読) [継承]
5. (英) Gene Expression Regulation (日) (読) [継承]
6. (英) Genetic Vectors (日) (読) [継承]
7. (英) Histidine (日) (読) [継承]
8. (英) Ligands (日) (読) [継承]
9. (英) Mitosporic Fungi (日) (読) [継承]
10. (英) Models, Molecular (日) (読) [継承]
11. (英) Molecular Sequence Data (日) (読) [継承]
12. (英) Mutagenesis, Site-Directed (日) (読) [継承]
13. (英) Oxidoreductases (日) (読) [継承]
14. (英) Oxidoreductases Acting on CH-CH Group Donors (日) (読) [継承]
15. (英) Recombinant Proteins (日) (読) [継承]
16. (英) Valine (日) (読) [継承]
発行所 (推奨): アメリカ化学会 [継承]
誌名 (必須): Biochemistry ([アメリカ化学会])
(pISSN: 0006-2960, eISSN: 1520-4995)

ISSN (任意): 0006-2960
ISSN: 0006-2960 (pISSN: 0006-2960, eISSN: 1520-4995)
Title: Biochemistry
Title(ISO): Biochemistry
Publisher: American Chemical Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 38 [継承]
(必須): 10 [継承]
(必須): 3034 3042 [継承]
都市 (任意):
年月日 (必須): 西暦 1999年 2月 17日 (平成 11年 2月 17日) [継承]
URL (任意):
DOI (任意): 10.1021/bi9819531    (→Scopusで検索) [継承]
PMID (任意): 10074356    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000079089300020 [継承]
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Atsushi Shimizu, Jung-Hee Kwon, Takashi Sasaki, Takanori Satoh, Nobuhiko Sakurai, Takeshi Sakurai, Shotaro Yamaguchi and Tatsuya Samejima : Myrothecium verrucaria Bilirubin Oxidase and Its Mutants for Potential Copper-Ligands., Biochemistry, Vol.38, No.10, 3034-3042, 1999.
欧文冊子 ● Atsushi Shimizu, Jung-Hee Kwon, Takashi Sasaki, Takanori Satoh, Nobuhiko Sakurai, Takeshi Sakurai, Shotaro Yamaguchi and Tatsuya Samejima : Myrothecium verrucaria Bilirubin Oxidase and Its Mutants for Potential Copper-Ligands., Biochemistry, Vol.38, No.10, 3034-3042, 1999.

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